Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ATSB10_RS11665 Genome accession   NZ_CP014841
Coordinates   2562974..2564032 (-) Length   352 a.a.
NCBI ID   WP_017463444.1    Uniprot ID   A0A160N255
Organism   Dyella thiooxydans strain ATSB10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2557974..2569032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATSB10_RS11645 (ATSB10_23920) - 2558916..2561072 (-) 2157 WP_063672978.1 type IV pilus secretin PilQ -
  ATSB10_RS11650 (ATSB10_23930) - 2561129..2561677 (-) 549 WP_063672979.1 pilus assembly protein PilP -
  ATSB10_RS11655 (ATSB10_23940) pilO 2561674..2562327 (-) 654 WP_063672980.1 type 4a pilus biogenesis protein PilO -
  ATSB10_RS11660 (ATSB10_23950) - 2562324..2562974 (-) 651 WP_063672981.1 PilN domain-containing protein -
  ATSB10_RS11665 (ATSB10_23960) pilM 2562974..2564032 (-) 1059 WP_017463444.1 pilus assembly protein PilM Machinery gene
  ATSB10_RS11670 (ATSB10_23970) - 2564284..2566824 (+) 2541 WP_063672982.1 penicillin-binding protein 1A -
  ATSB10_RS11675 (ATSB10_23980) - 2566896..2567567 (+) 672 WP_063672983.1 hypothetical protein -
  ATSB10_RS11680 (ATSB10_23990) - 2567646..2568941 (-) 1296 WP_063672984.1 citrate synthase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37950.57 Da        Isoelectric Point: 4.5827

>NTDB_id=174616 ATSB10_RS11665 WP_017463444.1 2562974..2564032(-) (pilM) [Dyella thiooxydans strain ATSB10]
MGLFTPKKPPLIGVDISSTAVKLLQLSQTGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRALARSGSKLKHACAA
VAGSAVITRVIPMSAELSEDDLEGQIQVEANQYIPYPIEEVSLDFEVLGPVRDNPDMNNILLAASRTENVDMRVAALDLG
GLSAKVIDVEAFALENAFAMLADQLNVGRDALVAVVDIGATMTTLSVLRNQRTIYSREQVFGGKQLTDEIMRRYGLSYEE
AGRAKRKGGLPESYETEALEPFKESLIQQISRLLQFFFAGSEYSKVDQVVLAGGCASIEGLGPMLEEQLGISCVVANPLA
RMSLSSRVQAQALAQDAPALMIAVGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=174616 ATSB10_RS11665 WP_017463444.1 2562974..2564032(-) (pilM) [Dyella thiooxydans strain ATSB10]
GTGGGTCTTTTCACACCCAAGAAGCCGCCGCTGATCGGCGTCGACATCAGTTCGACTGCCGTCAAGCTGCTGCAGCTGAG
CCAGACCGGAGGGCGTTACCGCGTGGAGCACTACGCGGTGGAGCCGTTGCCCCCGAATGCCGTCGTCGAGAAGAACATCG
TCGAGGTCGAGGCGGTCGGCGAGGCGATCCGTCGGGCGCTGGCCCGCTCGGGCTCCAAGCTCAAGCACGCCTGTGCCGCC
GTGGCCGGCTCGGCCGTGATCACCCGGGTCATCCCGATGTCCGCGGAGCTTTCCGAGGACGATCTGGAAGGCCAGATCCA
GGTGGAGGCGAACCAGTACATCCCTTACCCGATCGAGGAAGTGAGCCTCGATTTCGAGGTACTGGGGCCGGTTCGCGACA
ACCCGGACATGAACAACATCCTGCTCGCCGCGTCGCGGACCGAGAACGTGGACATGCGCGTGGCAGCGCTCGATCTCGGC
GGCCTGAGCGCCAAGGTGATCGACGTCGAGGCCTTTGCGCTGGAAAACGCCTTCGCGATGCTGGCCGACCAGTTGAACGT
GGGGCGCGACGCCCTGGTCGCCGTGGTCGACATCGGCGCCACCATGACCACGCTCTCCGTGCTGCGCAACCAGCGCACCA
TCTACTCCCGTGAGCAGGTGTTCGGCGGCAAGCAGCTGACCGACGAGATCATGCGTCGCTACGGACTCTCCTACGAGGAA
GCCGGACGGGCCAAGCGCAAGGGTGGCCTGCCGGAATCCTACGAGACCGAGGCACTGGAGCCGTTCAAGGAGTCGCTGAT
CCAGCAGATCAGCCGGCTGCTCCAGTTCTTCTTCGCCGGCAGCGAGTACAGCAAGGTGGACCAGGTGGTCCTGGCAGGCG
GGTGTGCCTCCATCGAGGGGCTCGGCCCGATGCTGGAGGAGCAGCTGGGCATCTCCTGCGTGGTGGCCAATCCGCTCGCA
CGCATGTCGCTGTCCTCGCGCGTGCAGGCCCAGGCTCTGGCCCAGGATGCCCCGGCGCTGATGATTGCGGTCGGCCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A160N255

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.874

98.864

0.523
  comM Acinetobacter nosocomialis M2

52.586

98.864

0.52
  comM Acinetobacter baylyi ADP1

50

99.432

0.497
  pilM Legionella pneumophila strain ERS1305867

42.776

100

0.429

Multiple sequence alignment