Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   BSR08_RS03850 Genome accession   NZ_CP018184
Coordinates   709406..711505 (-) Length   699 a.a.
NCBI ID   WP_017695065.1    Uniprot ID   -
Organism   Bacillus subtilis strain KH2     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 704406..716505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSR08_RS03820 (BSR08_03865) spo0E 704464..704721 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  BSR08_RS03825 (BSR08_03870) - 704807..705237 (+) 431 Protein_746 hypothetical protein -
  BSR08_RS03830 (BSR08_03875) kinD 705265..706785 (-) 1521 WP_031600444.1 sporulation kinase KinD -
  BSR08_RS03835 (BSR08_03880) mhqR 706978..707415 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  BSR08_RS03840 (BSR08_03885) motB 707455..708237 (-) 783 WP_014479633.1 flagellar motor protein MotB -
  BSR08_RS03845 (BSR08_03890) motA 708212..709021 (-) 810 WP_014479634.1 flagellar motor stator protein MotA -
  BSR08_RS03850 (BSR08_03895) clpE 709406..711505 (-) 2100 WP_017695065.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  BSR08_RS03855 (BSR08_03900) - 711873..712916 (+) 1044 WP_014479636.1 hypothetical protein -
  BSR08_RS03860 (BSR08_03905) queC 713229..713888 (+) 660 WP_014479637.1 7-cyano-7-deazaguanine synthase QueC -
  BSR08_RS03865 (BSR08_03910) queD 713881..714330 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  BSR08_RS03870 (BSR08_03915) queE 714323..715054 (+) 732 WP_014479638.1 7-carboxy-7-deazaguanine synthase QueE -
  BSR08_RS03875 (BSR08_03920) queF 715072..715569 (+) 498 WP_003218613.1 preQ(1) synthase -
  BSR08_RS03880 (BSR08_03925) ykvR 715890..716180 (+) 291 WP_014479639.1 YkvR family protein -
  BSR08_RS03885 (BSR08_03930) ykvS 716302..716487 (-) 186 WP_003232446.1 YkvS family protein -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77805.88 Da        Isoelectric Point: 5.1326

>NTDB_id=173343 BSR08_RS03850 WP_017695065.1 709406..711505(-) (clpE) [Bacillus subtilis strain KH2]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEKSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEEENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVQAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=173343 BSR08_RS03850 WP_017695065.1 709406..711505(-) (clpE) [Bacillus subtilis strain KH2]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAAAAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGCTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCAAAAGCCGGTCTCATTGACCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGGAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTAGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGTGATGTTCCAAACAAACTGAAAAACAAAGAGCTATATTTGCTTGATGTTGCATCCCTTGTTGCA
AACACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGTGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCATACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCATGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGAAGAAAATTACGAACTTGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATTGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAGGATGGCCGTCTGACAGACAGCCAAGGCAGAACTGTAAGCTTCAAAGACACAGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTGATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCTGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCAAGCGAAATAA

Domains

Predicted by InterProScan.

AAA lid domain (AAA_lid_9)

(253-352)

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.528

100

0.601
  clpC Lactococcus lactis subsp. cremoris KW2

56.284

100

0.589
  clpE Streptococcus pneumoniae TIGR4

54.924

100

0.567
  clpE Streptococcus pneumoniae Rx1

54.924

100

0.567
  clpE Streptococcus pneumoniae D39

54.924

100

0.567
  clpE Streptococcus pneumoniae R6

54.924

100

0.567
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Streptococcus pneumoniae Rx1

45.732

93.848

0.429
  clpC Streptococcus pneumoniae D39

45.732

93.848

0.429
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.847

89.7

0.429
  clpC Streptococcus thermophilus LMD-9

45.427

93.848

0.426
  clpC Streptococcus thermophilus LMG 18311

44.97

93.848

0.422
  clpC Streptococcus pneumoniae TIGR4

46.85

88.555

0.415
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

42.079

86.695

0.365

Multiple sequence alignment