Detailed information NTG0172622

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: BSG23_RS17220
• Genome accession: NZ_CP018115
• Coordinates: 3338061..3338720 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain MRSN346638
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3333061..3343720

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSG23_RS17190 (BSG23_16285)	ygiS	3333272..3334879 (-)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
BSG23_RS17195 (BSG23_16290)	-	3335017..3335991 (-)	975	WP_012478345.1	IS30-like element ISApl1 family transposase	-
BSG23_RS17200 (BSG23_16295)	mqsA	3336084..3336479 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
BSG23_RS17205 (BSG23_16300)	mqsR	3336481..3336777 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
BSG23_RS17210 (BSG23_16305)	ygiV	3336982..3337464 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
BSG23_RS17215 (BSG23_16310)	ygiW	3337517..3337909 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
BSG23_RS17220 (BSG23_16315)	ciaR	3338061..3338720 (+)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
BSG23_RS17225 (BSG23_16320)	qseC	3338717..3340066 (+)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
BSG23_RS17230 (BSG23_16325)	ygiZ	3340112..3340444 (-)	333	WP_023149630.1	DUF2645 family protein	-
BSG23_RS17235 (BSG23_16330)	mdaB	3340763..3341344 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
BSG23_RS17240 (BSG23_16335)	ygiN	3341375..3341689 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
BSG23_RS17245 (BSG23_16340)	parE	3341737..3343629 (-)	1893	WP_000195292.1	DNA topoisomerase IV subunit B	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=172622 BSG23_RS17220 WP_001221493.1 3338061..3338720(+) (ciaR) [Escherichia coli strain MRSN346638]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=172622 BSG23_RS17220 WP_001221493.1 3338061..3338720(+) (ciaR) [Escherichia coli strain MRSN346638]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365