Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AZF00_RS04275 Genome accession   NZ_CP014544
Coordinates   983291..984358 (+) Length   355 a.a.
NCBI ID   WP_008246192.1    Uniprot ID   -
Organism   Zhongshania aliphaticivorans strain SM-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 978291..989358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZF00_RS04265 (AZF00_04265) radA 979254..980621 (+) 1368 WP_008246190.1 DNA repair protein RadA -
  AZF00_RS04270 (AZF00_04270) - 980649..983120 (-) 2472 WP_008246191.1 penicillin-binding protein 1A -
  AZF00_RS04275 (AZF00_04275) pilM 983291..984358 (+) 1068 WP_008246192.1 pilus assembly protein PilM Machinery gene
  AZF00_RS04280 (AZF00_04280) - 984358..984918 (+) 561 WP_008246193.1 PilN domain-containing protein -
  AZF00_RS04285 (AZF00_04285) - 984931..985551 (+) 621 WP_008246194.1 type 4a pilus biogenesis protein PilO -
  AZF00_RS04290 (AZF00_04290) - 985548..986075 (+) 528 WP_008246195.1 pilus assembly protein PilP -
  AZF00_RS04295 (AZF00_04295) pilQ 986099..988300 (+) 2202 WP_008246196.1 type IV pilus secretin PilQ family protein -
  AZF00_RS04300 (AZF00_04300) aroK 988343..988843 (+) 501 WP_335338878.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38165.45 Da        Isoelectric Point: 4.2672

>NTDB_id=172438 AZF00_RS04275 WP_008246192.1 983291..984358(+) (pilM) [Zhongshania aliphaticivorans strain SM-2]
MLDGLFGGQGSRSLLGIDISSSAVKLLELSRSGEKYRVESYAVMSLPSQVVVEKNIADVEQVGDVVRAVHSRSRSKLKRA
AVAVPGSAIITKVLHMPAGLHENAMETQISLEADQYIPYPLDEVSLDFEVLGESETNPEMVDVMLVACRRDNVDTRVESL
EIAGITPKVVDVEAYAIERSFDLIAQQLGCDENTVVAIVDVGASMTSLSVIVNGETVYTRDQLFGGRQLTEEIQRRYGLT
VEEAGLAKKQGGLSDDYDSEVLAPFREAVIQQVSRSLQFFYSASQYSGVDIILLAGGVAAMQGLVELVQERLGIDTVIAN
PFAAMSVAPGVDATKLTSDAPAMLIACGLALRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=172438 AZF00_RS04275 WP_008246192.1 983291..984358(+) (pilM) [Zhongshania aliphaticivorans strain SM-2]
GTGCTAGATGGCTTGTTTGGCGGTCAGGGTTCCCGGAGTCTGCTCGGTATTGACATCAGTTCCAGTGCGGTTAAGTTACT
GGAGTTGAGCCGGAGTGGCGAGAAATATCGCGTAGAAAGCTACGCGGTAATGTCTCTGCCGTCGCAGGTTGTGGTCGAAA
AAAATATTGCTGATGTCGAGCAGGTGGGCGACGTTGTGCGTGCAGTGCACTCCCGATCTCGCAGCAAGCTGAAACGCGCC
GCGGTGGCAGTGCCTGGTTCGGCAATTATTACCAAAGTTCTGCACATGCCTGCGGGTCTGCATGAAAATGCCATGGAAAC
GCAGATTTCCCTCGAGGCTGATCAGTATATTCCTTACCCATTAGATGAGGTTTCTCTGGACTTTGAGGTCTTGGGTGAAT
CGGAAACCAACCCGGAAATGGTGGATGTGATGCTCGTGGCTTGTCGTCGCGATAACGTCGATACGCGGGTTGAGTCATTA
GAAATTGCTGGGATTACCCCAAAAGTGGTGGATGTCGAGGCCTACGCTATTGAGCGGAGTTTTGATCTTATCGCTCAGCA
GCTAGGCTGTGATGAGAATACGGTGGTGGCCATTGTCGATGTTGGCGCCAGTATGACCTCCCTCAGTGTGATCGTGAACG
GCGAAACAGTTTATACCCGCGACCAATTGTTTGGCGGTCGTCAGCTAACCGAAGAGATCCAGCGTCGCTATGGCTTGACG
GTGGAAGAAGCTGGTCTGGCCAAAAAGCAAGGCGGACTATCCGATGACTATGACAGTGAAGTCTTGGCTCCGTTCCGAGA
AGCGGTTATTCAACAGGTATCTAGGTCACTGCAATTCTTTTATTCGGCCAGTCAGTACAGCGGTGTCGATATTATTTTAT
TAGCTGGCGGTGTTGCCGCCATGCAGGGCTTGGTCGAGCTGGTCCAAGAGCGCTTGGGCATAGATACGGTTATTGCGAAC
CCGTTTGCGGCCATGTCGGTTGCACCGGGTGTAGACGCCACTAAATTGACCAGTGATGCCCCGGCCATGCTGATTGCCTG
CGGTTTGGCACTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.738

97.746

0.515
  comM Acinetobacter nosocomialis M2

52.738

97.746

0.515
  comM Acinetobacter baylyi ADP1

51.014

97.183

0.496
  pilM Legionella pneumophila strain ERS1305867

47.826

97.183

0.465

Multiple sequence alignment