Detailed information NTG0171808

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: JT25_RS12355
• Genome accession: NZ_CP014476
• Coordinates: 2694657..2695718 (+)
• Length: 353 a.a.
• NCBI ID: WP_036278118.1
• Uniprot ID: -
• Organism: Methylomonas denitrificans strain FJG1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2689657..2700718

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JT25_RS24750	-	2689789..2690004 (-)	216	WP_132325373.1	hypothetical protein	-
JT25_RS12345 (JT25_012345)	-	2690061..2691440 (+)	1380	WP_082769227.1	AmpG family muropeptide MFS transporter	-
JT25_RS24020	rpmE	2691809..2692009 (+)	201	WP_081607938.1	50S ribosomal protein L31	-
JT25_RS12350 (JT25_012350)	-	2692081..2694444 (-)	2364	WP_036278115.1	penicillin-binding protein 1A	-
JT25_RS12355 (JT25_012355)	pilM	2694657..2695718 (+)	1062	WP_036278118.1	pilus assembly protein PilM	Machinery gene
JT25_RS12360 (JT25_012360)	-	2695718..2696290 (+)	573	WP_036278121.1	PilN domain-containing protein	-
JT25_RS12365 (JT25_012365)	-	2696287..2696913 (+)	627	WP_062328763.1	type 4a pilus biogenesis protein PilO	-
JT25_RS12370 (JT25_012370)	-	2696910..2697467 (+)	558	WP_052142356.1	pilus assembly protein PilP	-
JT25_RS12375 (JT25_012375)	pilQ	2697470..2699710 (+)	2241	WP_036278124.1	type IV pilus secretin PilQ	-
JT25_RS25945	lptM	2699851..2699976 (+)	126	WP_081607937.1	LPS translocon maturation chaperone LptM	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 37923.40 Da
• Isoelectric Point: 4.7094

>NTDB_id=171808 JT25_RS12355 WP_036278118.1 2694657..2695718(+) (pilM) [Methylomonas denitrificans strain FJG1]
MSWLSRNPSAVLGVDISTAAVKLLELSRVGARYRVESYSVAPLPQDAIVDKNITNIEQIGAVIKAAVKQSGTRAKQASVA
VAGSSVMTKIISMPASLSDRDMEEQIMVEADQYIPYSLDEVNLDFEVQGVTKNNPDMVDVLLAASRRENIEDRVAALAYA
GLKAVVVDVEAFAMENAFSLLADQLPEGAGNMTVAIADIGATMTSLNILHEGKTVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYVTDVLEPFKKAMLQQIARSLQFFVSSSANRGVDALVLAGGCASIAGLDKLVERDLGIPSYIANPF
INMALSNRVKPQSLSNDTPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=171808 JT25_RS12355 WP_036278118.1 2694657..2695718(+) (pilM) [Methylomonas denitrificans strain FJG1]
ATGAGCTGGTTGAGCAGGAATCCGTCGGCTGTGCTGGGTGTGGATATTAGTACAGCTGCGGTTAAATTGTTGGAATTAAG
CCGGGTCGGTGCCCGCTACCGAGTAGAAAGCTACTCGGTAGCGCCATTGCCTCAGGATGCGATAGTCGATAAGAACATCA
CGAATATCGAGCAAATCGGCGCGGTAATAAAAGCGGCGGTGAAGCAATCCGGTACTCGTGCCAAGCAGGCGAGTGTCGCG
GTTGCGGGTTCGTCGGTGATGACCAAGATTATCTCGATGCCCGCCTCCTTATCCGATAGAGACATGGAAGAGCAGATCAT
GGTGGAGGCCGATCAGTACATTCCCTATTCGCTGGATGAGGTCAATCTCGATTTCGAGGTGCAGGGCGTCACTAAAAATA
ATCCGGATATGGTCGATGTGTTGTTGGCGGCTTCTCGGCGAGAAAACATTGAAGATCGGGTTGCCGCGCTGGCTTATGCC
GGCTTGAAGGCAGTTGTAGTCGATGTGGAAGCTTTCGCGATGGAAAATGCGTTTTCCTTGTTGGCGGATCAATTGCCGGA
AGGTGCCGGTAATATGACCGTCGCTATCGCCGACATCGGCGCAACCATGACATCACTGAACATTCTGCATGAAGGCAAAA
CTGTTTATACCCGAGAACAGGGCTTTGGCGGCAAGCAGCTAACCGAAGAGATTCAGCGACGCTACGGCCTATCTTACGAA
GAAGCTGGCTTGGCCAAGAAGCACGGCGGCTTGCCGGATAATTATGTCACGGATGTGTTGGAGCCTTTCAAAAAGGCCAT
GTTGCAGCAAATCGCCAGATCGTTGCAATTCTTCGTTTCCTCTAGCGCTAATCGCGGTGTGGATGCGTTGGTGCTTGCCG
GCGGCTGCGCATCAATCGCCGGATTGGACAAATTGGTGGAGCGCGATCTGGGTATTCCTTCCTACATTGCCAACCCCTTC
ATCAACATGGCGCTTTCCAATCGGGTTAAACCTCAAAGCCTGAGCAACGATACGCCAGCGATGATGATAGCGTGCGGTTT
AGCGTTAAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.695	100	0.518
comM	Acinetobacter nosocomialis M2	51.695	100	0.518
comM	Acinetobacter baylyi ADP1	50	100	0.501
pilM	Legionella pneumophila strain ERS1305867	40.793	100	0.408