Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AXF35_RS07750 Genome accession   NZ_CP014255
Coordinates   1675333..1677057 (+) Length   574 a.a.
NCBI ID   WP_027221709.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. pascullei strain F-4185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1670333..1682057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXF35_RS07730 (AXF35_09095) - 1670623..1673151 (+) 2529 WP_027221705.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  AXF35_RS07735 (AXF35_09090) - 1673153..1673722 (-) 570 WP_027221706.1 hypoxanthine-guanine phosphoribosyltransferase -
  AXF35_RS07740 (AXF35_09085) - 1673765..1674091 (-) 327 WP_027221707.1 DUF1820 family protein -
  AXF35_RS07745 (AXF35_09080) - 1674276..1675019 (-) 744 WP_027221708.1 methyltransferase domain-containing protein -
  AXF35_RS07750 (AXF35_09075) pilB 1675333..1677057 (+) 1725 WP_027221709.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AXF35_RS07755 (AXF35_09070) pilC 1677063..1678283 (+) 1221 WP_027221710.1 type II secretion system F family protein Machinery gene
  AXF35_RS07760 (AXF35_09065) - 1678327..1679190 (+) 864 WP_027221711.1 prepilin peptidase -
  AXF35_RS07765 (AXF35_09060) - 1679204..1680409 (-) 1206 WP_027221712.1 cyclic nucleotide-binding domain-containing protein -
  AXF35_RS07770 (AXF35_09055) - 1680521..1681114 (-) 594 WP_027221713.1 hypothetical protein -
  AXF35_RS07775 (AXF35_09050) - 1681130..1681585 (-) 456 WP_027221714.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62775.32 Da        Isoelectric Point: 5.5842

>NTDB_id=169820 AXF35_RS07750 WP_027221709.1 1675333..1677057(+) (pilB) [Legionella pneumophila subsp. pascullei strain F-4185]
MALATDEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKMSLVQYLVKNKILSAEQIALTAAQNFGVPMMDINCIEVDSI
PANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSSLIDNLLTAKESQGLSNYVE
DSGDLEGLEISTEDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLA
SRITARIKVMSNLDISERRIPQDGGFKMKISKARAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRSNFIK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKVVRDDFTNQG
LIELGFKESDLVNLKLYKAVGCDQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=169820 AXF35_RS07750 WP_027221709.1 1675333..1677057(+) (pilB) [Legionella pneumophila subsp. pascullei strain F-4185]
ATGGCTCTAGCTACAGATGAATATAGATTACAGGGAATTGGCCAGCTTCTAGTACTGGAAAAGTTGCTAGATAAAACCAA
AGCAATTGAGTTACATAAATTGGCTGCAGCAGAAAAAATGTCTTTAGTGCAATATCTTGTAAAAAATAAAATACTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCAATGATGGATATTAATTGCATTGAGGTGGACTCTATT
CCTGCCAATCTGGTTAATGAGAAATTGATTAAACGTCATGCTATGGTCCCCCTGTTTAGTCGCGGCACTAACTTGTACCT
TGCAACAGATGATCCTAGTAAGCAAGCTTCATTAAAGGAAATTCAATTTCATACCGGACTGAATACCCATGCTATAGTTG
TAGAAACAGATAAACTAAGTTCTTTGATTGATAACTTATTAACAGCAAAGGAAAGTCAGGGCTTATCGAACTATGTCGAA
GACTCAGGTGATCTGGAAGGTTTAGAAATAAGTACCGAGGATGAAGATCAGGATATTGATACAGCAACATCAGTTACTGA
TGATGCACCTATCGTAAAATTTGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGCGCTTCAGATATACATTTTGAGC
CTTATGAACGAGAATACCGGATCAGGTACAGGCAAGATGGTATTTTACATGAGGTAGCTACCCCGCCTGCAAGCCTTGCA
TCCCGTATTACGGCGCGTATCAAGGTAATGTCTAATTTGGATATATCAGAGAGGCGTATACCCCAGGATGGCGGCTTTAA
AATGAAAATTTCCAAGGCACGAGCTATTGATTTCAGAGTGAGCACCTGTCCAACCTCAGCCGGTGAAAAAGTGGTTATGC
GAGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGAGGTCAAATTTCATTAAA
GCAATTCAGCGCCCACAAGGGATGATTCTAGTCACAGGTCCTACAGGTAGTGGGAAAACAGTTACTTTATATACAGCATT
AAATATATTAAATACAATTGAAGTGAACATTTCAACAGCAGAGGATCCTGTTGAAATCAAGGTACCTGGCATCAATCAGG
TTAATATTAATCCTAAAGCTGGTCTAACCTTTTCCGGTGCATTGCGCTCGTTTCTAAGGCAAGACCCTGATATCATAATG
GTTGGTGAGATACGAGATCTTGAAACAGCTGAAATTGCTGTAAAAGCTGCGCAGACAGGGCATTTGGTTCTTTCTACACT
GCATACCAACAGTGCTGCTGAAACATTAAATCGTTTAGTGAACATGGGGATACCTACTTTTAACATTGCGAGTTCGGTTA
CATTAATTATTGCGCAAAGGTTGGCAAGAAAATTATGTAATCAATGTAAGGTTGTTCGCGATGACTTTACCAATCAAGGT
TTGATTGAATTAGGTTTCAAGGAATCTGATCTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGTGATCAATGCACAAG
TGGCTATCGTGGGCGGGTTGGTCTGTTTGAGGTTTTACCGATGACAAAAGAGCTAGGCCAGTTGATTATGTCAGGTGGTA
ACTCCCTGGATATCTTAAAGTTAGCCCAATCTGAAGGTATGCTGACTATCTTTCAATCGGGTATAGAAAAAGTCAAGGAA
GGGATCACAACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

96.341

100

0.963
  pilB Acinetobacter baumannii D1279779

50.97

98.78

0.503
  pilB Acinetobacter baylyi ADP1

49.231

100

0.502
  pilB Vibrio cholerae strain A1552

49.908

94.251

0.47
  pilB Vibrio campbellii strain DS40M4

47.397

97.038

0.46
  pilF Neisseria gonorrhoeae MS11

46.466

98.606

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

48.513

93.728

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.226

92.683

0.373

Multiple sequence alignment