Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AXE82_RS09595 Genome accession   NZ_CP014234
Coordinates   2175932..2176984 (+) Length   350 a.a.
NCBI ID   WP_062334119.1    Uniprot ID   A0A378QB27
Organism   Moraxella osloensis strain CCUG 350     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2170932..2181984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXE82_RS09585 (AXE82_09590) - 2172322..2172840 (+) 519 WP_062334113.1 DUF6586 family protein -
  AXE82_RS09590 (AXE82_09595) - 2172971..2175700 (-) 2730 WP_062334116.1 penicillin-binding protein 1A -
  AXE82_RS09595 (AXE82_09600) pilM 2175932..2176984 (+) 1053 WP_062334119.1 pilus assembly protein PilM Machinery gene
  AXE82_RS09600 (AXE82_09605) - 2176984..2177655 (+) 672 WP_062334123.1 PilN domain-containing protein -
  AXE82_RS09605 (AXE82_09610) - 2177658..2178419 (+) 762 WP_062334126.1 type 4a pilus biogenesis protein PilO -
  AXE82_RS09610 (AXE82_09615) - 2178406..2178939 (+) 534 WP_062334128.1 pilus assembly protein PilP -
  AXE82_RS09615 (AXE82_09620) pilQ 2178957..2181329 (+) 2373 WP_062334131.1 type IV pilus secretin family protein -
  AXE82_RS09620 (AXE82_09625) - 2181437..2181973 (+) 537 WP_062334134.1 shikimate kinase -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38379.82 Da        Isoelectric Point: 4.6466

>NTDB_id=169706 AXE82_RS09595 WP_062334119.1 2175932..2176984(+) (pilM) [Moraxella osloensis strain CCUG 350]
MRLFTKNNKLVVGVDITTTAIKMVEITRQQGLFHLRNYGIEPLPRGAVVDKNIVDIDAVSDALARLSRGRGFSSKHIATA
VSGSSVISKIIEMEADLTELERESRIRLDADQYIPYPLSEVNLDFEVIGPAATPDMCKVLLVASRTENVDQRVDAGALAG
LETKIVDVEAYAVERAFQLMVDSLPGQPQHVALIDIGHSQTTLYIAKDGEFVYSREQLFGGAQLTESIQSRYGLTFEEAM
INKRTLSLPDDYYTDVLMPFLDAIVQQITRSLQFYFSSSQNSQVEYILLGGGSASIAGLASMVEQKLGTRTGIANAFSNM
TINNHINTELLIMDAPSLMAACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=169706 AXE82_RS09595 WP_062334119.1 2175932..2176984(+) (pilM) [Moraxella osloensis strain CCUG 350]
GTGAGGTTATTTACAAAAAACAACAAGCTAGTCGTTGGGGTAGATATCACCACCACTGCGATAAAAATGGTTGAAATAAC
ACGGCAGCAAGGTCTATTTCACTTAAGAAATTACGGCATTGAACCGTTACCCCGCGGTGCAGTTGTGGATAAAAATATTG
TAGATATTGACGCGGTCAGTGACGCCCTCGCAAGATTATCCCGTGGTCGCGGTTTTTCTAGTAAACATATCGCGACAGCC
GTATCAGGGTCTTCGGTTATTAGCAAAATTATTGAGATGGAAGCGGATCTCACAGAGCTTGAAAGAGAATCTCGTATCCG
TCTGGATGCTGACCAATATATTCCCTATCCTTTATCCGAAGTGAATTTAGATTTTGAAGTGATAGGGCCTGCAGCCACGC
CTGATATGTGTAAGGTATTGTTAGTTGCCTCTCGCACTGAAAACGTCGACCAGCGTGTCGATGCAGGTGCTTTAGCGGGA
CTGGAAACTAAAATTGTGGATGTAGAGGCTTACGCAGTTGAACGTGCCTTTCAATTGATGGTCGATAGCTTGCCAGGTCA
ACCTCAGCATGTAGCCCTCATTGATATCGGGCATAGCCAAACCACGCTTTATATTGCAAAAGACGGGGAGTTTGTATACA
GCCGTGAACAGCTTTTTGGTGGCGCCCAGTTAACTGAAAGTATTCAAAGTCGTTATGGTTTGACATTCGAGGAGGCGATG
ATTAATAAGCGCACCCTGTCATTGCCAGATGATTACTATACGGATGTGTTAATGCCATTTTTAGATGCCATTGTGCAGCA
AATCACCCGCTCGCTGCAGTTTTATTTTTCATCCAGCCAAAATAGCCAAGTAGAGTATATTTTGTTGGGTGGGGGTAGTG
CCAGCATTGCGGGTCTAGCAAGCATGGTTGAACAAAAACTTGGTACTCGCACAGGTATTGCCAATGCATTTTCAAATATG
ACGATTAATAACCATATCAACACTGAATTACTGATTATGGACGCGCCAAGCTTGATGGCGGCTTGTGGCTTGGCGTTACG
GAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378QB27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.852

100

0.52
  comM Acinetobacter nosocomialis M2

50.997

100

0.511
  comM Acinetobacter baylyi ADP1

48.433

100

0.486
  pilM Legionella pneumophila strain ERS1305867

42.776

100

0.431

Multiple sequence alignment