Detailed information NTG0169140

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AWM80_RS12810
• Genome accession: NZ_CP014166
• Coordinates: 2481198..2481815 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis subsp. subtilis strain CU1050
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2476198..2486815

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AWM80_RS12785 (AWM80_12790)	rpsT	2476500..2476766 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
AWM80_RS12790 (AWM80_12795)	holA	2476781..2477824 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
AWM80_RS21675	-	2477864..2478013 (-)	150	WP_003229985.1	hypothetical protein	-
AWM80_RS12795 (AWM80_12800)	yqzM	2478054..2478188 (+)	135	WP_003229983.1	YqzM family protein	-
AWM80_RS12800 (AWM80_12805)	comEC	2478228..2480558 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AWM80_RS12805 (AWM80_12810)	comEB	2480562..2481131 (-)	570	WP_003229978.1	ComE operon protein 2	-
AWM80_RS12810 (AWM80_12815)	comEA	2481198..2481815 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
AWM80_RS12815 (AWM80_12820)	comER	2481899..2482720 (+)	822	WP_004398597.1	late competence protein ComER	-
AWM80_RS12820 (AWM80_12825)	yqeM	2482786..2483529 (-)	744	WP_003229973.1	class I SAM-dependent DNA methyltransferase	-
AWM80_RS12825 (AWM80_12830)	rsfS	2483526..2483882 (-)	357	WP_003229971.1	ribosome silencing factor	-
AWM80_RS12830 (AWM80_12835)	yqeK	2483900..2484460 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AWM80_RS12835 (AWM80_12840)	nadD	2484450..2485019 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
AWM80_RS12840 (AWM80_12845)	yhbY	2485031..2485321 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
AWM80_RS12845 (AWM80_12850)	aroE	2485315..2486157 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=169140 AWM80_RS12810 WP_004398514.1 2481198..2481815(-) (comEA) [Bacillus subtilis subsp. subtilis strain CU1050]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=169140 AWM80_RS12810 WP_004398514.1 2481198..2481815(-) (comEA) [Bacillus subtilis subsp. subtilis strain CU1050]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39