Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   AWM80_RS12800 Genome accession   NZ_CP014166
Coordinates   2478228..2480558 (-) Length   776 a.a.
NCBI ID   WP_009967776.1    Uniprot ID   A0AAE2SL11
Organism   Bacillus subtilis subsp. subtilis strain CU1050     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2473228..2485558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM80_RS12770 (AWM80_12775) yqxA 2473567..2473905 (-) 339 WP_004399162.1 YqxA family protein -
  AWM80_RS12775 (AWM80_12780) spoIIP 2473922..2475127 (-) 1206 WP_003229993.1 spore autolysin SpoIIP -
  AWM80_RS12780 (AWM80_12785) gpr 2475190..2476296 (-) 1107 WP_003229991.1 GPR endopeptidase -
  AWM80_RS12785 (AWM80_12790) rpsT 2476500..2476766 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  AWM80_RS12790 (AWM80_12795) holA 2476781..2477824 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  AWM80_RS21675 - 2477864..2478013 (-) 150 WP_003229985.1 hypothetical protein -
  AWM80_RS12795 (AWM80_12800) yqzM 2478054..2478188 (+) 135 WP_003229983.1 YqzM family protein -
  AWM80_RS12800 (AWM80_12805) comEC 2478228..2480558 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AWM80_RS12805 (AWM80_12810) comEB 2480562..2481131 (-) 570 WP_003229978.1 ComE operon protein 2 -
  AWM80_RS12810 (AWM80_12815) comEA 2481198..2481815 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  AWM80_RS12815 (AWM80_12820) comER 2481899..2482720 (+) 822 WP_004398597.1 late competence protein ComER -
  AWM80_RS12820 (AWM80_12825) yqeM 2482786..2483529 (-) 744 WP_003229973.1 class I SAM-dependent DNA methyltransferase -
  AWM80_RS12825 (AWM80_12830) rsfS 2483526..2483882 (-) 357 WP_003229971.1 ribosome silencing factor -
  AWM80_RS12830 (AWM80_12835) yqeK 2483900..2484460 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AWM80_RS12835 (AWM80_12840) nadD 2484450..2485019 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  AWM80_RS12840 (AWM80_12845) yhbY 2485031..2485321 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 776 a.a.        Molecular weight: 86701.36 Da        Isoelectric Point: 7.3497

>NTDB_id=169138 AWM80_RS12800 WP_009967776.1 2478228..2480558(-) (comEC) [Bacillus subtilis subsp. subtilis strain CU1050]
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIILIKTRHAFLIIVCFFSFILFFVLYAVTDSQNVSSYRQGTYQF
KAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKEQLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHI
HWNYSVTSIQNCSEPENFKYKVLSLRKHIISFTNSLLPPDSTGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKWRVRSATAICLSYIVLLLFNP
YHLFEAGFQLSFAVSFSLILSSSIFQQVKTSLGQLTIVSLIAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGA
VAGVLLLSLSASFGRLFFSWFDLLISWINRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLMVTGG
ICCTVMFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWREKQHPFSLGEKVLIPFLTAKG
IKQLDALILTHADQDHIGEAEILLKHHKVKRLVIPKGFVSEPKDEKVLQAAREEGVAIEEVKRGDVLQIKDLQFHVLSPE
APDPASKNNSSLVLWMETGGMSWILTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
NRYHHPHQKVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN

Nucleotide


Download         Length: 2331 bp        

>NTDB_id=169138 AWM80_RS12800 WP_009967776.1 2478228..2480558(-) (comEC) [Bacillus subtilis subsp. subtilis strain CU1050]
ATGCGTAATTCGCGCTTATTATTGCCTATGGCGGCAGCTTCGGCAACGGCTGGAATTACTGCCGCCGCTTATTTCCCCGC
TATTTTTCTTTTCATCCTCTTTCTCCTCATCATTTTAATCAAAACGAGGCACGCTTTTCTTATTATTGTTTGTTTCTTCT
CTTTTATATTGTTTTTTGTACTGTATGCAGTCACAGATTCTCAGAATGTCTCTTCCTATCGGCAGGGAACCTATCAATTC
AAGGCAGTGATTGACACTATTCCCAAAATTGACGGCGACCGTATGTCTATGATGGTTGAGACACCTGATAAGGAAAAATG
GGCTGCTGCGTATCGCATTCAGTCTGCTGGTGAAAAAGAACAGCTGTTATACATAGAACCAGGAATGTCATGTGAGTTGA
CTGGTACATTGGAAGAACCGAATCACGCAACTGTGCCGGGTGCATTTGATTATAACGAGTATCTTTATCGGCAGCATATT
CATTGGAACTACTCTGTCACGTCTATCCAAAACTGCAGCGAACCTGAAAATTTCAAGTACAAGGTGCTCAGCTTGAGAAA
ACATATCATATCATTCACAAACAGCCTTCTGCCTCCTGATTCGACAGGGATTGTACAGGCACTTACAGTTGGTGACAGAT
TTTACGTGGAGGATGAAGTGCTTACCGCGTATCAAAAGCTTGGTGTTGTCCATCTCTTGGCAATATCAGGACTCCACGTG
GGGATTTTGACAGCAGGTTTGTTTTATATCATGATCCGCCTTGGTATAACTAGAGAAAAGGCGTCAATTCTGTTGCTGTT
ATTTCTGCCGCTCTATGTGATGTTGACCGGCGCTGCTCCTTCAGTGCTACGCGCCGCTCTCATGTCGGGTGTTTACTTAG
CTGGAAGTCTTGTCAAATGGCGTGTCCGCTCTGCAACTGCAATTTGCCTTTCGTACATCGTCCTTCTGCTCTTCAATCCT
TATCATCTCTTTGAAGCCGGTTTTCAGCTATCGTTCGCCGTCAGTTTTTCTTTAATTCTATCCTCTTCTATTTTTCAGCA
GGTTAAAACCTCCTTGGGGCAGCTGACAATTGTATCACTCATCGCTCAGCTGGGCTCGCTTCCGATTCTCCTATATCATT
TTCATCAGTTTTCTATCATCAGCGTACCGATGAATATGTTGATGGTACCATTTTATACCTTCTGTATTTTGCCGGGAGCT
GTAGCAGGTGTTCTTCTATTAAGTCTTTCCGCTTCGTTTGGGAGATTGTTTTTCAGCTGGTTTGATTTATTGATAAGCTG
GATCAATAGGTTAATCACAAACATTGCAGATGTTGATGTGTTCACGATTATGATCGCACATCCTGCACCTGTTTTGCTTT
TTTTATTCACAGTCACGATCATCCTATTGCTTATGGCGATTGAAAAACGCTCCTTGTCGCAGTTGATGGTAACCGGAGGC
ATTTGCTGCACGGTGATGTTTCTGCTCTTTATATATCCGTGTCTTAGTTCCGAAGGAGAAGTGGATATGATAGATATTGG
ACAGGGTGACAGCATGTTTGTAGGTGCTCCGCATCAGCGGGGTCGTGTCTTAATTGATACCGGCGGCACTTTGTCTTACT
CGTCAGAGCCTTGGCGCGAAAAACAGCATCCGTTTTCACTGGGGGAAAAGGTGCTGATTCCGTTTTTAACTGCTAAGGGA
ATCAAGCAGCTTGACGCTTTAATTCTGACGCACGCTGACCAAGATCATATCGGAGAGGCGGAGATTCTGCTGAAGCATCA
TAAAGTAAAGCGCCTCGTGATTCCGAAAGGGTTCGTTTCTGAACCTAAAGATGAGAAAGTGCTGCAGGCAGCCAGGGAAG
AGGGAGTGGCAATTGAAGAGGTGAAGCGAGGCGATGTGTTGCAAATAAAGGATTTGCAGTTCCATGTACTGTCACCGGAA
GCACCTGATCCGGCAAGCAAAAATAATTCATCTCTCGTTCTGTGGATGGAGACGGGCGGTATGAGCTGGATCTTGACGGG
TGATCTGGAGAAAGAAGGGGAACAAGAGGTGATGAACGTGTTTCCGAATATAAAAGCAGATGTGTTAAAGGTGGGGCACC
ATGGGAGCAAAGGCTCTACCGGTGAAGAATTCATCCAACAGCTTCAGCCGAAAACGGCCATTATCTCAGCCGGGAAAAAC
AATCGGTACCATCATCCTCATCAAAAAGTTCTGCAACTATTACAGAGACATTCTATCCGCGTGCTGCGAACAGATCAAAA
CGGAACGATCCAATATAGATACAAAAACAGAGTTGGAACCTTTTCTGTCTATCCTCCATATGATACATCAGATATAACAG
AGACGAACTAA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-469)

Domain of unknown function (DUF4131) (DUF4131)

(20-170)

Metallo-beta-lactamase superfamily (Lactamase_B)

(507-718)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment