Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PcP3B5_RS12090 Genome accession   NZ_CP014158
Coordinates   2715239..2716441 (+) Length   400 a.a.
NCBI ID   WP_176707187.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain P3B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2710239..2721441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PcP3B5_RS12065 (PcP3B5_24660) - 2710251..2711531 (-) 1281 WP_043266605.1 OprD family porin -
  PcP3B5_RS12070 (PcP3B5_24670) - 2712370..2713569 (-) 1200 WP_082816294.1 MFS transporter -
  PcP3B5_RS12075 (PcP3B5_24680) - 2713839..2714396 (-) 558 WP_061561354.1 putative glycolipid-binding domain-containing protein -
  PcP3B5_RS12080 (PcP3B5_24690) - 2714393..2714755 (-) 363 WP_009619514.1 MmcQ/YjbR family DNA-binding protein -
  PcP3B5_RS12085 (PcP3B5_24700) - 2714769..2715047 (-) 279 WP_009619516.1 peptidylprolyl isomerase -
  PcP3B5_RS12090 (PcP3B5_24710) pilU 2715239..2716441 (+) 1203 WP_176707187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PcP3B5_RS32130 (PcP3B5_24720) - 2716681..2716812 (+) 132 WP_374046694.1 hypothetical protein -
  PcP3B5_RS12095 (PcP3B5_24730) - 2717019..2717960 (+) 942 WP_061561355.1 MBL fold metallo-hydrolase -
  PcP3B5_RS12100 (PcP3B5_24750) - 2718423..2718695 (+) 273 WP_009619536.1 hypothetical protein -
  PcP3B5_RS12105 (PcP3B5_24760) - 2719196..2720029 (-) 834 WP_061561356.1 oxidoreductase -
  PcP3B5_RS12110 (PcP3B5_24770) - 2720123..2721067 (+) 945 WP_145923889.1 AraC family transcriptional regulator -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 43903.04 Da        Isoelectric Point: 6.0779

>NTDB_id=168931 PcP3B5_RS12090 WP_176707187.1 2715239..2716441(+) (pilU) [Pseudomonas citronellolis strain P3B5]
MEIQAMLRILSSQDGSDLYLSTGAPPCAKFNGVLKPLSSEPLKAGDVARIADAIMDEEQKAEFERELEMNLAISVAGVGR
FRINIFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLHRDNPAAAQTQAASAQPATPAAPAVEASSWGLELALEEIKAEDEEDKKGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=168931 PcP3B5_RS12090 WP_176707187.1 2715239..2716441(+) (pilU) [Pseudomonas citronellolis strain P3B5]
ATGGAAATCCAAGCGATGCTGCGGATCCTGTCCAGCCAGGACGGTTCCGACCTTTACCTTTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAGCGAACCGCTGAAGGCCGGCGACGTGGCGCGCATCGCCGACGCCA
TCATGGACGAGGAACAGAAGGCCGAATTCGAGCGCGAGCTGGAGATGAACCTGGCGATCTCGGTCGCCGGCGTCGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCGTTGTTCGA
AGACCTGAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGGCACATCATTACCATCGAGGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACCCTGCACGCCAATAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCACAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGCCGCGCCGCGGTGGAGGTGCTGCTGGGCACCGCGACCATCAGCGACATCATCA
AGCGCGGCGACTTCAGTTCGATCAAGGAGATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAAGGCGCGATCAGCGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGGCTGAA
GCTCAAGCTGCACCGGGACAACCCGGCCGCGGCCCAGACCCAGGCCGCCAGCGCCCAGCCGGCAACCCCGGCCGCGCCCG
CGGTGGAAGCCTCCAGCTGGGGCCTGGAACTGGCCCTGGAAGAGATCAAGGCCGAGGACGAGGAAGACAAGAAGGGCGGT
TGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.695

87.75

0.497
  pilU Acinetobacter baylyi ADP1

53.409

88

0.47
  pilU Vibrio cholerae strain A1552

52.125

88.25

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.157

95.25

0.382

Multiple sequence alignment