Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AUP74_RS05725 Genome accession   NZ_CP014143
Coordinates   1326897..1327973 (+) Length   358 a.a.
NCBI ID   WP_069946737.1    Uniprot ID   A0A1C9W655
Organism   Microbulbifer aggregans strain CCB-MM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1321897..1332973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AUP74_RS05715 (AUP74_01156) - 1323429..1323911 (+) 483 WP_069946735.1 chemotaxis protein CheW -
  AUP74_RS05720 (AUP74_01157) - 1324090..1326747 (-) 2658 WP_069946736.1 penicillin-binding protein 1A -
  AUP74_RS05725 (AUP74_01158) pilM 1326897..1327973 (+) 1077 WP_069946737.1 pilus assembly protein PilM Machinery gene
  AUP74_RS05730 (AUP74_01159) - 1327976..1328566 (+) 591 WP_069946738.1 PilN domain-containing protein -
  AUP74_RS05735 (AUP74_01160) - 1328579..1329199 (+) 621 WP_069946739.1 type 4a pilus biogenesis protein PilO -
  AUP74_RS05740 (AUP74_01161) - 1329196..1329726 (+) 531 WP_069946740.1 pilus assembly protein PilP -
  AUP74_RS05745 (AUP74_01162) pilQ 1329726..1331939 (+) 2214 WP_193427352.1 type IV pilus secretin PilQ -
  AUP74_RS05750 (AUP74_01163) aroK 1331939..1332463 (+) 525 WP_083261092.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38937.31 Da        Isoelectric Point: 4.3426

>NTDB_id=168693 AUP74_RS05725 WP_069946737.1 1326897..1327973(+) (pilM) [Microbulbifer aggregans strain CCB-MM1]
MGMLPFLNKGPKAMLGLDISSTAVKLLELSRNGDKYRVECYAVEPLPPNAVVDKNINDVGATAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLEMAADLNEDALEAQIALEADQYIPYPLDEVNLDFEVQGPSDKGEGQVEVLLAACRSENIEQRVSAL
AEAELQAAVVDVEAYAIERSYTLMERQFPPQEQLVVAVIDIGATMTALSVMVDGKTVYTREQLFGGRQLTDEIQRRYGLS
AEEAGLAKRQGGSGLPDDYYPEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDYILLGGGVAAMEGLADLVGEKLGKPTMV
ANPFQDMTVASRVNRQALANEAPSLMIAAGLAMREKEY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=168693 AUP74_RS05725 WP_069946737.1 1326897..1327973(+) (pilM) [Microbulbifer aggregans strain CCB-MM1]
ATGGGGATGCTCCCTTTTCTCAATAAGGGCCCGAAGGCCATGTTGGGGCTAGATATTAGCTCCACTGCGGTCAAGTTACT
GGAGCTAAGTCGCAACGGTGACAAATACCGGGTCGAATGTTACGCCGTCGAGCCCCTGCCTCCCAATGCGGTAGTCGACA
AAAACATCAATGACGTGGGTGCGACCGCGGAAGCAATCCGCAAAGTGGTGCGCCGTTCGAAGACTCGCCTGCGCCAGGCC
GCGGTAGCGGTGTCCGGCTCTGCCGTGATCACCAAGACCCTGGAAATGGCTGCGGATCTCAATGAGGACGCGCTCGAGGC
GCAGATTGCCCTCGAGGCCGACCAGTACATCCCCTATCCGCTGGATGAGGTCAACCTCGACTTTGAGGTGCAGGGCCCAT
CGGACAAGGGTGAAGGTCAGGTGGAAGTCCTGCTGGCCGCCTGTCGCAGCGAGAACATCGAGCAGCGAGTTTCCGCTCTT
GCAGAAGCCGAGCTTCAGGCCGCTGTGGTGGATGTGGAAGCGTACGCCATCGAGCGCTCTTACACGCTGATGGAGCGGCA
GTTCCCGCCCCAGGAGCAGCTGGTGGTGGCCGTGATCGATATCGGCGCCACTATGACCGCGCTCTCCGTGATGGTGGATG
GCAAGACCGTCTACACCCGCGAGCAGCTGTTCGGCGGTCGCCAGTTGACCGATGAAATCCAGCGTCGCTATGGCCTCTCC
GCCGAGGAGGCGGGTCTGGCCAAGCGTCAGGGCGGCAGTGGCCTGCCAGACGACTATTACCCCGAGGTCCTGGAGCCGTT
CCGTGACGCGGTGGTCCAGCAAGTGACCCGCTCCCTGCAGTTCTTCTACTCCTCTACTTCCTACAGTGATGTCGACTACA
TCCTGCTCGGTGGCGGCGTGGCTGCCATGGAGGGGCTGGCGGACCTGGTGGGGGAGAAACTGGGTAAGCCGACCATGGTG
GCAAACCCCTTCCAGGACATGACCGTGGCTTCCCGGGTCAATCGCCAGGCGCTTGCCAATGAGGCCCCCTCGCTGATGAT
CGCGGCGGGGCTAGCGATGCGCGAGAAGGAGTACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C9W655

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

98.324

0.506
  comM Acinetobacter nosocomialis M2

51.42

98.324

0.506
  comM Acinetobacter baylyi ADP1

47.443

98.324

0.466
  pilM Legionella pneumophila strain ERS1305867

44.101

99.441

0.439

Multiple sequence alignment