Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AL543_RS08765 Genome accession   NZ_CP014043
Coordinates   414524..415540 (-) Length   338 a.a.
NCBI ID   WP_061051688.1    Uniprot ID   -
Organism   Vibrio mimicus strain FDAARGOS_112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409524..420540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL543_RS08740 (AL543_08005) aroK 410407..410931 (-) 525 WP_000818614.1 shikimate kinase AroK -
  AL543_RS08745 (AL543_08010) pilQ 411118..412833 (-) 1716 WP_061053027.1 type IV pilus secretin PilQ Machinery gene
  AL543_RS08750 (AL543_08015) pilP 412859..413377 (-) 519 WP_005524360.1 pilus assembly protein PilP Machinery gene
  AL543_RS08755 (AL543_08020) pilO 413367..413957 (-) 591 WP_000135849.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL543_RS08760 (AL543_08025) pilN 413950..414534 (-) 585 WP_061051687.1 PilN domain-containing protein Machinery gene
  AL543_RS08765 (AL543_08030) pilM 414524..415540 (-) 1017 WP_061051688.1 type IV pilus assembly protein PilM Machinery gene
  AL543_RS08770 (AL543_08035) - 415682..418159 (+) 2478 WP_061051689.1 penicillin-binding protein 1A -
  AL543_RS08775 (AL543_08040) oxyR 418226..419119 (-) 894 WP_061051690.1 DNA-binding transcriptional regulator OxyR -
  AL543_RS08780 (AL543_08045) - 419259..419990 (+) 732 WP_061051691.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37544.26 Da        Isoelectric Point: 7.6008

>NTDB_id=167873 AL543_RS08765 WP_061051688.1 414524..415540(-) (pilM) [Vibrio mimicus strain FDAARGOS_112]
MGKTLVTGIDIGHHSIKAVVLKPMGDTYALMGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDNA
VISKVLQIDSDLERREQEFAIYQAFSHQSPFPVEDLSLDFVKVAEKNIARSSTSTFQVYATKKEVVESRLQASKKAGFEP
ILMDVQVHSLLHLWQLASRAYRRPDWMLIDIGHTQSSLCLDFAEKMPFYKDVPLGTRVLGAEPSSTPVLGDSLMLDPTQR
FVNEFVDKVARQIQLFTSVHGAQSLGGLWLSGGGATIAGLEEALHQRLSLPCEVLNPFSLFKTNVSRRKRQVADGQRFST
AAGLALRGLAWLESEHAA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=167873 AL543_RS08765 WP_061051688.1 414524..415540(-) (pilM) [Vibrio mimicus strain FDAARGOS_112]
ATGGGTAAAACATTAGTTACGGGTATTGATATCGGCCACCACAGCATAAAAGCCGTAGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTATGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGGTTGCCCTTATTTAGTCACAAAGTCGCGATTGCAATTCCGGATAACGCT
GTAATTAGCAAAGTATTACAAATAGATAGCGATCTTGAACGTCGAGAACAGGAATTTGCCATCTATCAGGCCTTCTCTCA
TCAATCTCCATTTCCCGTTGAAGATCTTAGCCTCGATTTCGTCAAAGTGGCGGAAAAAAATATCGCGCGCAGTAGTACAA
GCACCTTCCAAGTCTATGCCACGAAAAAAGAAGTAGTGGAAAGCCGCTTACAAGCGAGTAAGAAAGCGGGGTTTGAGCCG
ATTTTGATGGATGTGCAAGTCCATAGCTTGCTCCATTTATGGCAGCTCGCTAGCCGTGCCTATCGACGTCCGGATTGGAT
GTTAATTGATATTGGGCATACCCAGAGTTCATTGTGCCTTGATTTCGCAGAAAAAATGCCTTTTTACAAAGATGTTCCAC
TGGGTACGCGAGTGTTGGGTGCAGAGCCGAGCAGCACTCCCGTATTAGGCGATTCATTAATGCTGGACCCCACTCAACGT
TTTGTTAATGAATTCGTGGATAAGGTTGCGCGCCAAATTCAATTGTTTACCTCCGTACATGGAGCTCAAAGTCTTGGCGG
TTTATGGCTTTCCGGCGGCGGTGCAACTATTGCTGGACTTGAGGAGGCGTTACACCAACGTTTATCACTTCCCTGTGAAG
TGTTGAATCCATTCTCACTATTCAAAACGAATGTCTCAAGACGCAAGCGCCAAGTGGCTGATGGGCAGCGCTTTAGCACT
GCCGCTGGCCTTGCATTGCGTGGTTTAGCATGGTTGGAGAGTGAACATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-217)

Type IV pilus assembly protein PilM; (PilM_2)

(239-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

85.799

100

0.858
  pilM Vibrio campbellii strain DS40M4

49.112

100

0.491

Multiple sequence alignment