Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AL543_RS08745 Genome accession   NZ_CP014043
Coordinates   411118..412833 (-) Length   571 a.a.
NCBI ID   WP_061053027.1    Uniprot ID   -
Organism   Vibrio mimicus strain FDAARGOS_112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406118..417833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL543_RS08725 (AL543_07990) - 406868..407701 (-) 834 WP_061051684.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  AL543_RS08730 (AL543_07995) - 407876..409240 (-) 1365 Protein_367 AAA family ATPase -
  AL543_RS08735 (AL543_08000) aroB 409294..410379 (-) 1086 WP_061051686.1 3-dehydroquinate synthase -
  AL543_RS08740 (AL543_08005) aroK 410407..410931 (-) 525 WP_000818614.1 shikimate kinase AroK -
  AL543_RS08745 (AL543_08010) pilQ 411118..412833 (-) 1716 WP_061053027.1 type IV pilus secretin PilQ Machinery gene
  AL543_RS08750 (AL543_08015) pilP 412859..413377 (-) 519 WP_005524360.1 pilus assembly protein PilP Machinery gene
  AL543_RS08755 (AL543_08020) pilO 413367..413957 (-) 591 WP_000135849.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL543_RS08760 (AL543_08025) pilN 413950..414534 (-) 585 WP_061051687.1 PilN domain-containing protein Machinery gene
  AL543_RS08765 (AL543_08030) pilM 414524..415540 (-) 1017 WP_061051688.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62391.45 Da        Isoelectric Point: 4.5760

>NTDB_id=167869 AL543_RS08745 WP_061053027.1 411118..412833(-) (pilQ) [Vibrio mimicus strain FDAARGOS_112]
MRNGLKTYVAQTWLTLWAGLALCASSLAISAEPASANQLENIDFRVNKDKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVQSVEVFRKEPSTQLIATIEGEFKHDYTLKGKYLEVVISKLKADEKPQPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARMAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGTISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGTIESNLWQQGLLAEDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SDTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATTVSFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=167869 AL543_RS08745 WP_061053027.1 411118..412833(-) (pilQ) [Vibrio mimicus strain FDAARGOS_112]
ATGAGAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTATGGGCAGGCTTGGCACTTTGTGCTTCTTCCTT
GGCGATTAGCGCTGAACCAGCTTCCGCCAATCAACTAGAGAACATTGATTTTCGGGTCAATAAAGACAAAGCGGCTGTGT
TGATTGTCGAATTGGCTTCGCCATCAGCGGTGGTGGATGTACAAAAAGTCCAAGAAGGGCTGAGTATTGAGCTCCTGAAA
ACCGATGTGGCAGATGACAAACTCTATTTATTGGATGTGAAGGATTTCTCTACACCAGTACAAAGCGTAGAAGTCTTCCG
TAAAGAACCCAGTACCCAGCTTATTGCAACCATCGAAGGTGAGTTTAAGCATGACTACACCTTAAAAGGGAAATACCTTG
AGGTGGTAATCAGTAAGCTCAAAGCAGACGAGAAGCCTCAGCCTAAAAGTATTTTGGAAAAAGAGGGCAAGCTGATCTCG
ATTAACTTCCAAGACATTCCAGTGCGTAACGTACTGCAGTTGATTGCTGATTATAACGGTTTCAACCTCGTTGTTTCAGA
CTCAGTCTCGGGCAATCTGACTTTGCGTTTGGATGGTGTACCTTGGCAGCAAGTGCTGGATATTATCCTGCAGGTTAAAG
GGCTTGATAAACGCGTTGATGGCAATGTAATCCTGATTGCGCCAAAAGAAGAGCTGGATTTGCGTGAGAAACAAGCGTTA
GAAAAAGCACGTATGGCAGAAGAGTTAGGTGATTTAAAATCTGAAATCATCAAAATTAATTTTGCCAAAGCATCTGATAT
CGCAGCAATGATTGGTGGTGAAGGTAACGTCAATATGCTGTCTGAGCGTGGCACGATCAGTATTGATGAACGTACTAACT
CACTGCTGATTCGTGAATTACCCGATAATATCGCGGTGATCCGTGAAATCATCGAATCGCTAGATATTCCGGTCAAGCAA
GTCCAGATTGAAGCACGTATTGTCACCGTAAAAGAAGGTAACTTGGATGAGCTTGGTGTGCGTTGGGGTGTTATGTCAAC
CAACGGCAGCCACTCTGTGGGTGGAACAATTGAAAGTAACTTATGGCAACAAGGACTGCTGGCGGAAGATGAGTTTCCAG
TTGATGAATTTTTGAATGTTAACTTAGCTTCTACGTCCGCTAATGCCTCTTCTATCGCTTTCCAAGTTGCTAAGTTGGGC
TCAGATACCTTATTGGATTTGGAGTTATCTGCGTTACAAAATGAATCGAAGGCAGAAATCATTTCTAGCCCTCGTTTGAT
TACGACCAATAAACAGCCTGCGTATATTGAACAAGGTACTGAAATTCCTTATCTCGAATCCTCATCTAGTGGTGCAACAA
CCGTATCGTTCAAGAAAGCAGTATTGAGCTTGAAAGTGACACCACAAATTACGCCGGATAATCGTTTGGTACTGGATTTG
AGTGTGACTCAGGACCGTCGTGGCGAAACGGTGAAAACAGGAACAGGTGAAGCGGTATCGATCGATACCCAGCGTATTGG
TACACAAGTTCTGGTCAATAATGGTGAAACCGTCGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCAGTGGATA
AGGTGCCGTTGCTAGGTGATCTCCCGGTTTTAGGTGCGCTGTTTAGACGAACTTACGAACAAATGGGCAAAAGTGAGCTG
CTGATTTTCGTTACACCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(181-229)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)

Bacterial type II and III secretion system protein (Secretin)

(411-569)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

95.447

100

0.954
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

95.447

100

0.954
  pilQ Vibrio campbellii strain DS40M4

78.584

96.497

0.758
  pilQ Legionella pneumophila strain ERS1305867

43.173

94.921

0.41
  pilQ Legionella pneumophila strain Lp02

43.173

94.921

0.41
  pilQ Pseudomonas aeruginosa PAK

39.012

100

0.401
  comQ Acinetobacter baylyi ADP1

39.446

100

0.399
  pilQ Acinetobacter baumannii D1279779

38.24

100

0.396
  pilQ Acinetobacter baumannii strain A118

38.24

100

0.396

Multiple sequence alignment