Detailed information NTG0167620

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: A4W86_RS01975
• Genome accession: NZ_CP017272
• Coordinates: 424700..425707 (+)
• Length: 335 a.a.
• NCBI ID: WP_016264643.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain TMW 1.1239
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 419700..430707

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4W86_RS01955 (A4W86_01940)	hexB	420619..422580 (+)	1962	WP_035144579.1	DNA mismatch repair endonuclease MutL	Machinery gene
A4W86_RS01960 (A4W86_01945)	-	422580..423125 (+)	546	WP_016264640.1	Maf family protein	-
A4W86_RS01965 (A4W86_01950)	-	423138..423701 (+)	564	WP_016264641.1	TIGR00730 family Rossman fold protein	-
A4W86_RS01970 (A4W86_01955)	ruvA	424076..424687 (+)	612	WP_016264642.1	Holliday junction branch migration protein RuvA	Machinery gene
A4W86_RS01975 (A4W86_01960)	ruvB	424700..425707 (+)	1008	WP_016264643.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
A4W86_RS01980 (A4W86_01965)	queA	425722..426753 (+)	1032	WP_011374077.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
A4W86_RS01985 (A4W86_01970)	-	426780..427301 (+)	522	WP_016264644.1	GNAT family protein	-
A4W86_RS01990 (A4W86_01975)	arcA	427509..428744 (+)	1236	WP_016264645.1	arginine deiminase	-
A4W86_RS01995 (A4W86_01980)	argF	428770..429786 (+)	1017	WP_011374080.1	ornithine carbamoyltransferase	-

Sequence

Protein

• Length: 335 a.a.
• Molecular weight: 37247.68 Da
• Isoelectric Point: 4.8413

>NTDB_id=167620 A4W86_RS01975 WP_016264643.1 424700..425707(+) (ruvB) [Latilactobacillus sakei strain TMW 1.1239]
MADERIVSAENDDFAEASIEKTLRPQVLAQYIGQDRVKNELAVYIEAAKKREESLDHVLLYGPPGLGKTTLAMVIANELQ
VQIRTTSGPAIERPGDLVALLNELQPGDVLFIDEIHRLPKMVEELLYSAMEDFYIDIVVGQGPTAHPVHFPLPPFTLIGA
TTRAGLLSAPLRDRFGIVEHMAYYTEADLMDIVQRSAGVFNMSIVPDGALEIARRSRGTPRIANRLLKRTRDYAQVADQN
TIDQAIADHALSQLQVDIRGLDGVDRKILQMMIDYYQGGPVGLKTIAANIGEENETIEEVYEPYLLQIGFLKRTQRGRLV
TPAGYAHLGMPYPEK

Nucleotide

• Length: 1008 bp

>NTDB_id=167620 A4W86_RS01975 WP_016264643.1 424700..425707(+) (ruvB) [Latilactobacillus sakei strain TMW 1.1239]
ATGGCAGATGAGCGAATTGTTTCAGCCGAAAACGATGATTTTGCTGAAGCAAGCATTGAAAAAACATTGCGGCCACAAGT
CCTTGCACAATATATCGGTCAAGATCGGGTTAAAAATGAATTAGCGGTCTATATTGAAGCGGCCAAAAAACGGGAAGAGT
CCCTCGATCACGTTTTACTTTATGGCCCACCAGGTTTGGGGAAAACCACGTTAGCGATGGTGATTGCCAACGAGTTGCAA
GTCCAGATTCGAACGACAAGTGGCCCCGCAATCGAGCGGCCTGGAGATTTAGTGGCGTTATTAAACGAGTTACAACCTGG
GGATGTTTTATTTATTGATGAAATTCATCGCTTACCTAAGATGGTTGAAGAATTACTTTATTCAGCGATGGAAGATTTCT
ACATCGATATTGTGGTTGGTCAAGGGCCTACCGCACATCCCGTCCATTTTCCATTACCACCATTCACATTGATTGGCGCG
ACGACGCGGGCGGGCTTATTGTCTGCGCCATTGCGAGATCGTTTCGGGATTGTGGAACACATGGCTTATTACACGGAGGC
TGATTTGATGGACATTGTGCAACGCTCAGCCGGCGTCTTTAATATGTCGATTGTGCCAGATGGTGCGTTAGAAATCGCAC
GTCGCTCGCGAGGGACGCCGCGGATTGCCAATCGGTTGTTGAAACGAACTCGAGATTACGCACAAGTGGCTGACCAAAAT
ACGATTGATCAGGCGATTGCTGATCACGCATTAAGTCAGCTGCAAGTTGATATTCGGGGCTTAGACGGTGTCGATCGCAA
AATCCTCCAGATGATGATTGATTATTATCAAGGCGGACCAGTCGGTTTAAAAACCATTGCCGCCAATATTGGTGAAGAAA
ACGAAACAATTGAAGAAGTTTACGAGCCTTATTTACTGCAAATTGGCTTTTTAAAACGAACGCAAAGAGGCCGGCTGGTA
ACGCCGGCTGGTTATGCCCATTTAGGCATGCCTTATCCAGAAAAATAG

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (260-328)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	64.653	98.806	0.639
ruvB	Streptococcus pneumoniae R6	64.688	95.522	0.618
ruvB	Streptococcus pneumoniae D39	64.688	95.522	0.618
ruvB	Streptococcus pneumoniae TIGR4	64.375	95.522	0.615
ruvB	Synechocystis sp. PCC 6803	58.28	93.731	0.546
ruvB	Helicobacter pylori 26695	51.829	97.91	0.507