Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BHW77_RS02370 Genome accession   NZ_CP017251
Coordinates   481045..481512 (-) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli strain NADC 5570/86-24/6564 isolate wild     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476045..486512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHW77_RS02345 (BHW77_02345) rpsG 476186..476656 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  BHW77_RS02350 (BHW77_02350) fusA 476753..478867 (+) 2115 WP_000124700.1 elongation factor G -
  BHW77_RS02355 (BHW77_02355) tuf 478938..480122 (+) 1185 WP_000031783.1 elongation factor Tu -
  BHW77_RS02360 (BHW77_02360) bfd 480305..480499 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  BHW77_RS02365 (BHW77_02365) bfr 480572..481048 (+) 477 WP_000675516.1 bacterioferritin -
  BHW77_RS02370 (BHW77_02370) pilD 481045..481512 (-) 468 WP_000381746.1 prepilin peptidase Machinery gene
  BHW77_RS02380 (BHW77_02380) rpsJ 481891..482202 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  BHW77_RS02385 (BHW77_02385) rplC 482235..482864 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  BHW77_RS02390 (BHW77_02390) rplD 482875..483480 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  BHW77_RS02395 (BHW77_02395) rplW 483477..483779 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  BHW77_RS02400 (BHW77_02400) rplB 483797..484618 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  BHW77_RS02405 (BHW77_02405) rpsS 484635..484913 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  BHW77_RS02410 (BHW77_02410) rplV 484928..485260 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  BHW77_RS02415 (BHW77_02415) rpsC 485278..485979 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  BHW77_RS02420 (BHW77_02420) rplP 485992..486402 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=167384 BHW77_RS02370 WP_000381746.1 481045..481512(-) (pilD) [Escherichia coli strain NADC 5570/86-24/6564 isolate wild]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=167384 BHW77_RS02370 WP_000381746.1 481045..481512(-) (pilD) [Escherichia coli strain NADC 5570/86-24/6564 isolate wild]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCCTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387

Multiple sequence alignment