Detailed information NTG0167302

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AL533_RS12805
• Genome accession: NZ_CP014019
• Coordinates: 2672655..2673773 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain FDAARGOS_129
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2667655..2678773

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AL533_RS12790 (AL533_12790)	-	2669821..2670663 (-)	843	WP_006581385.1	undecaprenyl-diphosphate phosphatase	-
AL533_RS12795 (AL533_12795)	-	2670771..2671463 (-)	693	WP_016804433.1	YggS family pyridoxal phosphate-dependent enzyme	-
AL533_RS12800 (AL533_12800)	pilT	2671590..2672627 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
AL533_RS12805 (AL533_12805)	pilU	2672655..2673773 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AL533_RS12810 (AL533_12810)	fur	2673843..2674280 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
AL533_RS12815 (AL533_12815)	-	2674392..2674790 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AL533_RS12820 (AL533_12820)	-	2674846..2675169 (-)	324	WP_002052062.1	RnfH family protein	-
AL533_RS12825 (AL533_12825)	-	2675169..2676236 (-)	1068	WP_004710227.1	hypothetical protein	-
AL533_RS12835 (AL533_12835)	-	2676389..2676844 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AL533_RS12840 (AL533_12840)	-	2677097..2677945 (-)	849	WP_016804434.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=167302 AL533_RS12805 WP_002052019.1 2672655..2673773(+) (pilU) [Acinetobacter nosocomialis strain FDAARGOS_129]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=167302 AL533_RS12805 WP_002052019.1 2672655..2673773(+) (pilU) [Acinetobacter nosocomialis strain FDAARGOS_129]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGTATTGATACGGACTCTTTCGA
AATTGCTTTAAAAAATACATTACGACAAGCACCAGATGTGATCTTAATTGGTGAAATCCGCTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
GCAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTCGACTTATATAAAGCGGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAGCTTGCAAATGAAGGTCCTGATCAAATTACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403