Detailed information NTG0166937

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: APT60_RS26615
• Genome accession: NZ_CP013989
• Coordinates: 5801167..5802231 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5796167..5807231

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
APT60_RS26590 (APT60_26590)	aroK	5796702..5797220 (-)	519	WP_003095833.1	shikimate kinase AroK	-
APT60_RS26595 (APT60_26595)	pilQ	5797232..5799376 (-)	2145	WP_003161249.1	type 4a pilus secretin PilQ	Machinery gene
APT60_RS26600 (APT60_26600)	pilP	5799430..5799954 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
APT60_RS26605 (APT60_26605)	pilO	5799951..5800574 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
APT60_RS26610 (APT60_26610)	pilN	5800571..5801167 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
APT60_RS26615 (APT60_26615)	pilM	5801167..5802231 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
APT60_RS26620 (APT60_26620)	-	5802416..5804887 (+)	2472	WP_059349813.1	penicillin-binding protein 1A	-
APT60_RS26625 (APT60_26625)	-	5804992..5806260 (-)	1269	WP_059349814.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=166937 APT60_RS26615 WP_003110888.1 5801167..5802231(-) (pilM) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=166937 APT60_RS26615 WP_003110888.1 5801167..5802231(-) (pilM) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGCCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466