Detailed information NTG0166854

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: APT59_RS03120
• Genome accession: NZ_CP013987
• Coordinates: 669319..670383 (+)
• Length: 354 a.a.
• NCBI ID: WP_059313506.1
• Uniprot ID: A0AAX3KW63
• Organism: Pseudomonas oryzihabitans strain USDA-ARS-USMARC-56511
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 664319..675383

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
APT59_RS03105 (APT59_03105)	-	664469..665296 (+)	828	WP_059313503.1	thermonuclease family protein	-
APT59_RS03110 (APT59_03110)	-	665409..666677 (+)	1269	WP_059313504.1	malic enzyme-like NAD(P)-binding protein	-
APT59_RS03115 (APT59_03115)	-	666731..669151 (-)	2421	WP_059313505.1	penicillin-binding protein 1A	-
APT59_RS03120 (APT59_03120)	pilM	669319..670383 (+)	1065	WP_059313506.1	pilus assembly protein PilM	Machinery gene
APT59_RS03125 (APT59_03125)	-	670384..670971 (+)	588	WP_059313507.1	PilN domain-containing protein	-
APT59_RS03130 (APT59_03130)	pilO	670968..671621 (+)	654	WP_059313508.1	type 4a pilus biogenesis protein PilO	-
APT59_RS03135 (APT59_03135)	-	671618..672136 (+)	519	WP_059313509.1	pilus assembly protein PilP	-
APT59_RS03140 (APT59_03140)	pilQ	672136..674385 (+)	2250	WP_059313510.1	type IV pilus secretin PilQ	Machinery gene
APT59_RS03145 (APT59_03145)	aroK	674391..674909 (+)	519	WP_059313511.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37811.20 Da
• Isoelectric Point: 4.6278

>NTDB_id=166854 APT59_RS03120 WP_059313506.1 669319..670383(+) (pilM) [Pseudomonas oryzihabitans strain USDA-ARS-USMARC-56511]
MLGLFRKKANTLLGIDVSSTSVKLIELSRAGGRYRVEAYAVEPLPANAVVEKNIAELEGVGLAIGRVLVKARTSARSAAV
AVSGSAVIAKTIEMDAGLSDDELENQIKLEADQYIPYPLDEVAIDFEVQGPSPRNAERVEVLLAACRKENVEVREAALAL
AGISAKVVDVEAYALERAYQLLANQLGGHVDDLTVAVVDIGATMTTLSVLHNGKTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYQSEVLTPFREAVVQQVARSLQFFFAAGQYNDVDYILLAGGTASISGLDRLIQQKIGTPTVLANP
FADMTVSNKVNAAALAGDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=166854 APT59_RS03120 WP_059313506.1 669319..670383(+) (pilM) [Pseudomonas oryzihabitans strain USDA-ARS-USMARC-56511]
GTGCTAGGGCTATTCAGAAAGAAGGCGAATACGCTGCTGGGTATCGACGTCAGTTCGACATCCGTGAAACTCATCGAGTT
GAGCCGTGCAGGCGGTCGCTATCGGGTGGAAGCCTATGCGGTCGAACCCCTCCCGGCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTCGAAGGCGTGGGCCTGGCCATCGGCCGGGTGCTGGTCAAGGCCCGCACTTCCGCGCGTAGCGCCGCCGTG
GCTGTTTCCGGTTCGGCAGTGATCGCCAAGACCATCGAGATGGATGCCGGCCTGTCTGATGACGAGCTGGAAAACCAGAT
CAAGCTGGAAGCCGACCAGTACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAGGTGCAGGGGCCGTCGCCGC
GCAATGCCGAGCGCGTCGAGGTGCTGCTGGCTGCCTGCCGAAAGGAAAACGTCGAGGTCCGCGAGGCGGCCCTGGCGCTG
GCCGGCATTTCGGCCAAGGTGGTCGACGTCGAGGCCTATGCGCTGGAGCGGGCCTACCAGCTACTGGCCAATCAACTTGG
CGGGCATGTCGACGACCTGACCGTGGCGGTGGTCGACATCGGCGCCACCATGACCACGCTGAGCGTGCTGCACAACGGCA
AGACCATCTATACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTTACCGAGGAGATCCAGCGACGCTATGGGTTGTCGGTG
GAGGAGGCGGGCCTGGCCAAGAAACAGGGCGGGCTGCCGGACGACTATCAGAGCGAGGTACTGACGCCTTTCCGCGAGGC
CGTGGTACAGCAGGTGGCCCGTTCGCTGCAGTTTTTCTTCGCGGCTGGGCAATACAATGATGTGGACTACATCTTGCTTG
CGGGTGGGACCGCGTCAATTTCCGGACTCGACCGCTTGATCCAGCAGAAAATCGGCACCCCCACCGTCCTGGCCAATCCC
TTCGCCGACATGACCGTCAGCAACAAGGTCAATGCGGCGGCCCTGGCCGGCGATGCCCCGGCGCTGATGATCGCCTGCGG
CCTGGCCATGAGGAGTTTCGACTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.062	100	0.571
comM	Acinetobacter nosocomialis M2	57.062	100	0.571
comM	Acinetobacter baylyi ADP1	55.085	100	0.551
pilM	Legionella pneumophila strain ERS1305867	44.35	100	0.444