Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FORC41_RS20875 Genome accession   NZ_CP016628
Coordinates   4001642..4002082 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain FORC_041     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3996642..4007082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC41_RS20850 (FORC41_3846) aroP 3997640..3999013 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  FORC41_RS20855 (FORC41_3847) ampE 3999056..3999910 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  FORC41_RS20860 (FORC41_3848) ampD 3999907..4000458 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC41_RS20865 (FORC41_3849) nadC 4000546..4001439 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC41_RS20875 (FORC41_3850) pilA 4001642..4002082 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  FORC41_RS20880 (FORC41_3851) pilB 4002092..4003477 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  FORC41_RS20885 (FORC41_3852) hofC 4003467..4004669 (+) 1203 WP_000157234.1 protein transport protein HofC -
  FORC41_RS20890 (FORC41_3853) guaC 4004704..4005747 (-) 1044 WP_001217338.1 GMP reductase -
  FORC41_RS26580 - 4005903..4005947 (-) 45 WP_120795372.1 protein YacM -
  FORC41_RS20895 (FORC41_3854) coaE 4005972..4006592 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=160977 FORC41_RS20875 WP_000360895.1 4001642..4002082(+) (pilA) [Escherichia coli strain FORC_041]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=160977 FORC41_RS20875 WP_000360895.1 4001642..4002082(+) (pilA) [Escherichia coli strain FORC_041]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment