Detailed information NTG0160425

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACA40_RS02070
• Genome accession: NZ_CP013183
• Coordinates: 443308..444372 (+)
• Length: 354 a.a.
• NCBI ID: WP_003341394.1
• Uniprot ID: A0A0P9GH14
• Organism: Pseudomonas syringae pv. lapsa strain ATCC 10859
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 438308..449372

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACA40_RS02060 (ACA40_02060)	-	439169..440437 (+)	1269	WP_003316648.1	malic enzyme-like NAD(P)-binding protein	-
ACA40_RS02065 (ACA40_02065)	-	440657..443098 (-)	2442	WP_370683520.1	penicillin-binding protein 1A	-
ACA40_RS02070 (ACA40_02070)	pilM	443308..444372 (+)	1065	WP_003341394.1	pilus assembly protein PilM	Machinery gene
ACA40_RS02075 (ACA40_02075)	-	444372..444953 (+)	582	WP_057407103.1	PilN domain-containing protein	-
ACA40_RS02080 (ACA40_02080)	pilO	444950..445573 (+)	624	WP_003316652.1	type 4a pilus biogenesis protein PilO	-
ACA40_RS02085 (ACA40_02085)	pilP	445570..446097 (+)	528	WP_003316653.1	type 4a pilus biogenesis lipoprotein PilP	-
ACA40_RS02090 (ACA40_02090)	pilQ	446145..448250 (+)	2106	WP_024649426.1	type IV pilus secretin PilQ	Machinery gene
ACA40_RS02095 (ACA40_02095)	aroK	448255..448773 (+)	519	WP_003341387.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38134.68 Da
• Isoelectric Point: 4.7090

>NTDB_id=160425 ACA40_RS02070 WP_003341394.1 443308..444372(+) (pilM) [Pseudomonas syringae pv. lapsa strain ATCC 10859]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=160425 ACA40_RS02070 WP_003341394.1 443308..444372(+) (pilM) [Pseudomonas syringae pv. lapsa strain ATCC 10859]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCATTGCCGGCCAACGCGGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTGCTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTACTGGCTGCCTGCCGCAAGGAAAACGTCGAAGTACGCGAGGCCGCGCTGGCACTG
GCCGGGCTGACCGCACGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGTTCGTTCGGTTTGCTGGCGGCGCAACTGGG
CAATGGCCACGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGTGGGCGCCAGTTGACTGACGAAATCCAGCGCCGCTACGGGCTCTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGATTACGTCAGCGAGGTGCTCGAGCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGATCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCAGTGGATCACATCATGCTGG
CAGGCGGCACGGCCTCGATTTCAGGGCTTGAGCATCTGATCCAGCGGCGCATCGGCACGCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCACTGAGCTCCAAGGTAAATGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCGTGCGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0P9GH14

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.65	100	0.557
comM	Acinetobacter nosocomialis M2	55.65	100	0.557
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469