Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   BB344_RS04155 Genome accession   NZ_CP016497
Coordinates   907537..908547 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain UPEC 26-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 902537..913547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BB344_RS04130 (BB344_04130) nudB 904260..904712 (+) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -
  BB344_RS04135 (BB344_04135) yebC 904741..905481 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  BB344_RS04140 (BB344_04140) ruvC 905516..906037 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  BB344_RS04145 (BB344_04145) yebB 906039..906641 (-) 603 WP_001024947.1 YebB family permuted papain-like enzyme -
  BB344_RS28835 yobI 906713..906778 (+) 66 WP_072123646.1 stress response small protein YobI -
  BB344_RS04150 (BB344_04150) ruvA 906917..907528 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  BB344_RS04155 (BB344_04155) ruvB 907537..908547 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  BB344_RS04160 (BB344_04160) znuB 908694..909479 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  BB344_RS04165 (BB344_04165) znuC 909476..910231 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  BB344_RS04170 (BB344_04170) znuA 910310..911242 (+) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  BB344_RS04175 (BB344_04175) mepM 911258..912580 (+) 1323 WP_042042898.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=160404 BB344_RS04155 WP_000568522.1 907537..908547(+) (ruvB) [Escherichia coli strain UPEC 26-1]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=160404 BB344_RS04155 WP_000568522.1 907537..908547(+) (ruvB) [Escherichia coli strain UPEC 26-1]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCTGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment