Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ACT75_RS05480 Genome accession   NZ_CP012959
Coordinates   1138725..1140134 (+) Length   469 a.a.
NCBI ID   WP_005554456.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 624     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1133725..1145134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT75_RS05455 (ACT75_05450) - 1133752..1136319 (-) 2568 WP_005549313.1 penicillin-binding protein 1A -
  ACT75_RS05460 (ACT75_05455) - 1136453..1137262 (+) 810 WP_005549310.1 hypothetical protein -
  ACT75_RS05465 (ACT75_05460) - 1137273..1137791 (+) 519 WP_005549308.1 PilN domain-containing protein -
  ACT75_RS05470 (ACT75_05465) - 1137788..1138312 (+) 525 WP_005543969.1 hypothetical protein -
  ACT75_RS05475 (ACT75_05470) - 1138312..1138704 (+) 393 WP_005549304.1 hypothetical protein -
  ACT75_RS05480 (ACT75_05475) comE 1138725..1140134 (+) 1410 WP_005554456.1 type IV pilus secretin PilQ Machinery gene
  ACT75_RS05485 (ACT75_05480) aroK 1140348..1140875 (+) 528 WP_005543963.1 shikimate kinase AroK -
  ACT75_RS05490 (ACT75_05485) aroB 1140899..1141987 (+) 1089 WP_005543960.1 3-dehydroquinate synthase -
  ACT75_RS05495 (ACT75_05490) - 1141990..1142844 (+) 855 WP_005543958.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACT75_RS11850 (ACT75_05495) - 1143083..1143712 (-) 630 WP_230806697.1 ABC transporter six-transmembrane domain-containing protein -
  ACT75_RS13075 (ACT75_05500) - 1143661..1143963 (-) 303 WP_005543955.1 ABC transporter six-transmembrane domain-containing protein -
  ACT75_RS05510 (ACT75_05505) - 1143963..1144406 (-) 444 WP_005543953.1 MarR family winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 51834.58 Da        Isoelectric Point: 7.1243

>NTDB_id=158602 ACT75_RS05480 WP_005554456.1 1138725..1140134(+) (comE) [Aggregatibacter actinomycetemcomitans strain 624]
MKSILSRWLKCGLFFGCFLSLTVQAEQNPVFSIRLKQAPLVPTLQQLALAHNTNLIIDDELQGTVSLQLENVDLDQLFRS
VAKIKQLDLWQENGIYYFIKGDTNTKFAGKMEEPFPLSLPMAEEPAQLTTATIKLHFAKASEVMKSLTGGSGSLLSPNGS
ITFDDRSNLLLIQDEPRSVRNIKKLIKELDKPIEQIAIEARIVTINDESLKELGVRWGIFNPTENAHRIGGSLAGNGFDN
LVNQLNVNFPTTTTPAGSVALQVAKINGRLLDLELTALERENNVEIIASPRLLTTNKKSASIKQGTELPYILVNEKSGSQ
SVEFREAVLGLEVTPHISQDKQILLDLIVSQNSPGSRVAYGLGEVVSIDKQEINTQVFAKDGETIVLGGVFSDTITKGTD
KVPVLGDIPGIKHLFSKESERHQKRELVIFVTPHILRKGETPDAFKQKRIGVNEQIMAKGENKKDLNRK

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=158602 ACT75_RS05480 WP_005554456.1 1138725..1140134(+) (comE) [Aggregatibacter actinomycetemcomitans strain 624]
ATGAAATCGATTCTTTCCCGATGGCTAAAGTGCGGTCTGTTTTTCGGGTGTTTTTTAAGTCTTACAGTGCAGGCGGAGCA
AAATCCGGTGTTTTCCATTCGGCTGAAACAGGCACCACTGGTTCCGACTTTGCAGCAGTTGGCATTAGCCCACAATACCA
ATTTGATTATTGACGACGAGCTGCAAGGTACGGTGTCGTTACAGTTGGAAAACGTGGATTTGGATCAGCTGTTTCGTTCT
GTGGCAAAAATTAAACAACTGGATTTATGGCAGGAAAACGGCATTTATTATTTCATTAAAGGCGACACCAACACGAAATT
TGCCGGCAAAATGGAAGAGCCTTTCCCTCTTTCACTGCCCATGGCGGAAGAACCGGCACAGCTGACCACGGCGACCATTA
AATTACACTTTGCCAAAGCGTCGGAAGTGATGAAATCCCTCACCGGGGGGAGTGGTTCCCTGTTGTCGCCTAACGGCAGT
ATCACTTTCGACGATCGTAGCAATTTGTTGCTGATTCAGGATGAACCGCGCTCCGTCCGCAACATAAAAAAGCTGATTAA
GGAACTGGATAAACCCATTGAGCAAATCGCCATTGAAGCGCGCATTGTCACCATTAACGATGAAAGCCTGAAAGAACTGG
GCGTGCGCTGGGGGATTTTCAACCCGACGGAAAACGCCCATCGTATCGGTGGCAGTTTGGCGGGCAACGGCTTTGATAAC
CTTGTCAATCAGTTAAACGTAAATTTTCCCACGACCACGACACCGGCAGGCTCTGTGGCGTTACAGGTTGCGAAAATCAA
CGGGCGACTTTTAGACTTAGAATTGACCGCACTTGAACGGGAAAATAATGTGGAAATCATCGCAAGCCCACGCCTGCTAA
CGACAAACAAAAAAAGCGCCAGCATCAAACAGGGGACGGAATTACCCTATATTCTGGTGAACGAAAAAAGCGGTTCGCAA
AGCGTGGAATTTCGTGAGGCGGTACTAGGTTTGGAAGTCACGCCGCACATTTCGCAGGATAAACAAATTCTGTTAGATCT
TATCGTCAGCCAAAACTCGCCGGGCAGCCGTGTGGCATACGGTTTGGGCGAAGTGGTTTCTATTGATAAACAGGAGATTA
ATACACAAGTCTTCGCCAAGGACGGCGAAACCATTGTGCTGGGCGGTGTATTTAGCGATACCATCACCAAAGGCACCGAT
AAAGTGCCGGTGCTGGGTGACATTCCGGGTATTAAACATTTATTCAGCAAGGAAAGTGAACGCCATCAAAAACGCGAGCT
GGTGATTTTTGTCACACCACATATTTTGCGTAAAGGCGAAACCCCGGACGCTTTCAAACAAAAGCGAATCGGGGTAAATG
AGCAAATCATGGCAAAAGGGGAGAATAAAAAGGACCTAAATAGGAAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(277-437)

Bacterial type II/III secretion system short domain (Secretin_N)

(130-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

72.098

95.522

0.689
  comE Haemophilus influenzae 86-028NP

71.205

95.522

0.68
  comE Glaesserella parasuis strain SC1401

53.089

93.177

0.495
  pilQ Vibrio campbellii strain DS40M4

41.244

92.537

0.382
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.589

91.258

0.38
  pilQ Vibrio cholerae strain A1552

41.589

91.258

0.38

Multiple sequence alignment