Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACT74_RS07000 Genome accession   NZ_CP012958
Coordinates   1491577..1492029 (+) Length   150 a.a.
NCBI ID   WP_005586498.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain VT1169     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1486577..1497029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT74_RS06985 (ACT74_06980) pnp 1487534..1489663 (-) 2130 WP_061870041.1 polyribonucleotide nucleotidyltransferase -
  ACT74_RS06990 (ACT74_06985) nanQ 1489957..1490427 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  ACT74_RS06995 (ACT74_06990) ampD 1490901..1491455 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACT74_RS07000 (ACT74_06995) pilA 1491577..1492029 (+) 453 WP_005586498.1 pilin Machinery gene
  ACT74_RS07005 (ACT74_07000) - 1492056..1493463 (+) 1408 Protein_1378 GspE/PulE family protein -
  ACT74_RS07010 (ACT74_07005) pilC 1493456..1494679 (+) 1224 WP_005567968.1 type II secretion system F family protein Machinery gene
  ACT74_RS07015 (ACT74_07010) - 1494676..1495365 (+) 690 WP_005547547.1 prepilin peptidase -
  ACT74_RS07020 (ACT74_07015) coaE 1495415..1496038 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  ACT74_RS07025 (ACT74_07020) yacG 1496028..1496240 (+) 213 WP_005567966.1 DNA gyrase inhibitor YacG -
  ACT74_RS07030 (ACT74_07025) - 1496240..1496512 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15547.96 Da        Isoelectric Point: 9.1384

>NTDB_id=158581 ACT74_RS07000 WP_005586498.1 1491577..1492029(+) (pilA) [Aggregatibacter actinomycetemcomitans strain VT1169]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGVITVTGKGSLDKISYTLTASGTAASGVSWNAACGNNADIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=158581 ACT74_RS07000 WP_005586498.1 1491577..1492029(+) (pilA) [Aggregatibacter actinomycetemcomitans strain VT1169]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATCGC
TATTGTTGCTATTCTCGCCACCGTAGCCGTACCATCTTATCAAAATTACACTAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCCCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AACGGCATTCAAGCAGATGTTGCAGATGCTTCGAAACAAAAATATGTGAAATCCACTGCGGTCAAAGGCGGCGTCATCAC
CGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACGGCTGCCAGCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGACATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.319

92

0.573
  pilA Haemophilus influenzae Rd KW20

59.028

96

0.567
  pilA Glaesserella parasuis strain SC1401

53.846

95.333

0.513

Multiple sequence alignment