Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AOT14_RS13795 Genome accession   NZ_CP012900
Coordinates   3128665..3129723 (-) Length   352 a.a.
NCBI ID   WP_054668759.1    Uniprot ID   -
Organism   Stenotrophomonas acidaminiphila strain ZAC14D2_NAIMI4_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3123665..3134723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT14_RS13770 (AOT14_28200) - 3123727..3124737 (-) 1011 WP_054667350.1 MoxR family ATPase -
  AOT14_RS13775 (AOT14_28210) - 3124852..3126744 (-) 1893 WP_054667352.1 type IV pilus secretin PilQ family protein -
  AOT14_RS13780 (AOT14_28220) - 3126766..3127302 (-) 537 WP_054667353.1 pilus assembly protein PilP -
  AOT14_RS13785 (AOT14_28230) pilO 3127302..3127979 (-) 678 WP_054667357.1 type 4a pilus biogenesis protein PilO -
  AOT14_RS13790 (AOT14_28240) - 3127976..3128665 (-) 690 WP_054667359.1 PilN domain-containing protein -
  AOT14_RS13795 (AOT14_28250) pilM 3128665..3129723 (-) 1059 WP_054668759.1 pilus assembly protein PilM Machinery gene
  AOT14_RS13800 (AOT14_28260) - 3129941..3132373 (+) 2433 WP_054667361.1 penicillin-binding protein 1A -
  AOT14_RS13805 (AOT14_28270) - 3132452..3133078 (+) 627 WP_054667363.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37395.95 Da        Isoelectric Point: 4.4316

>NTDB_id=158124 AOT14_RS13795 WP_054668759.1 3128665..3129723(-) (pilM) [Stenotrophomonas acidaminiphila strain ZAC14D2_NAIMI4_2]
MGLIPKSQPPIIGVDISSTAVKLLQLARSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVARSGTRAKGAAAA
VAGSAVITKVIPMPADLDEADLEAQVELEAVNYIPYPIEEVSLDFEVLGPVPNNPEMVQVLLAASRSENVELRQSALELG
GLVAKVMDVEAFAIENAFALVASELPVASDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEVEVLEPFKEATVQQIGRLLQFFYAGSEFNRVDHIVLAGGCASLPGLPVMVEEQLGVSTSVANPLA
QMTLGQKVQAHALAQDAPALMIATGLALRGFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=158124 AOT14_RS13795 WP_054668759.1 3128665..3129723(-) (pilM) [Stenotrophomonas acidaminiphila strain ZAC14D2_NAIMI4_2]
GTGGGGCTTATCCCAAAGAGTCAACCACCGATCATCGGTGTTGACATCAGCTCGACTGCGGTAAAGCTGCTTCAGCTTGC
CCGCAGCGGCAATCGTTTTCGCGTGGAACATTACGCCGTGGAACCACTTCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TCGAGGTCGAGGCGGTCGGCGAGGCCATACGCCGGGCCGTGGCGCGCTCGGGAACCCGGGCCAAGGGGGCGGCTGCCGCG
GTCGCCGGTTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGACCTGGACGAGGCCGACCTGGAAGCCCAGGTCGA
GCTGGAGGCGGTGAACTACATCCCGTATCCGATCGAGGAAGTGAGCCTGGATTTCGAGGTGCTCGGGCCGGTGCCCAACA
ACCCGGAGATGGTCCAGGTGCTGTTGGCGGCCTCGCGCTCGGAGAACGTCGAACTGCGCCAGTCGGCGCTGGAGCTGGGC
GGGCTCGTGGCCAAGGTCATGGACGTGGAAGCGTTCGCGATCGAGAACGCCTTCGCGCTGGTGGCCAGCGAACTGCCGGT
GGCCAGTGACGGCGTGGTCGCGCTGGTGGACATCGGCGCCACCATGACCACGCTCAACGTCCTGCGTGGTGGGCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTCACCGACGAGGTGATGCGCCGCTACGGGCTGAGCTACGAGGAG
GCCGGGCTGGCCAAGCGCCAGGGCGGGCTGCCGGAGAGTTACGAGGTGGAAGTGCTGGAGCCGTTCAAGGAGGCCACGGT
CCAGCAGATCGGCCGCCTGTTGCAGTTCTTCTATGCCGGCAGCGAGTTCAATCGCGTCGATCACATCGTGCTGGCCGGTG
GCTGCGCTTCGCTGCCCGGGCTGCCGGTGATGGTCGAGGAACAACTGGGGGTGAGCACGTCGGTGGCCAATCCGCTGGCG
CAGATGACGCTGGGACAGAAGGTGCAGGCGCATGCGCTGGCCCAGGATGCCCCGGCGCTGATGATCGCCACCGGCCTGGC
CCTGCGGGGGTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50

100

0.5
  comM Acinetobacter nosocomialis M2

50

100

0.5
  comM Acinetobacter baylyi ADP1

50.292

97.159

0.489
  pilM Legionella pneumophila strain ERS1305867

46.571

99.432

0.463

Multiple sequence alignment