Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   AK973_RS02860 Genome accession   NZ_CP012680
Coordinates   679999..681063 (+) Length   354 a.a.
NCBI ID   WP_003196973.1    Uniprot ID   A0A0Q7AAJ5
Organism   Pseudomonas brassicacearum strain LBUM300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 674999..686063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AK973_RS02850 (AK973_0556) - 675832..677100 (+) 1269 WP_003196970.1 malic enzyme-like NAD(P)-binding protein -
  AK973_RS02855 (AK973_0557) - 677346..679790 (-) 2445 WP_371916716.1 penicillin-binding protein 1A -
  AK973_RS02860 (AK973_0558) comM 679999..681063 (+) 1065 WP_003196973.1 pilus assembly protein PilM Machinery gene
  AK973_RS02865 (AK973_0559) - 681063..681629 (+) 567 WP_003196975.1 PilN domain-containing protein -
  AK973_RS02870 (AK973_0560) pilO 681626..682249 (+) 624 WP_003196977.1 type 4a pilus biogenesis protein PilO -
  AK973_RS02875 (AK973_0561) - 682246..682773 (+) 528 WP_003196979.1 pilus assembly protein PilP -
  AK973_RS02880 (AK973_0562) pilQ 682787..684874 (+) 2088 WP_060613200.1 type IV pilus secretin PilQ Machinery gene
  AK973_RS02885 (AK973_0563) aroK 684879..685397 (+) 519 WP_003196983.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38084.64 Da        Isoelectric Point: 4.9070

>NTDB_id=156315 AK973_RS02860 WP_003196973.1 679999..681063(+) (comM) [Pseudomonas brassicacearum strain LBUM300]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKAKTPLRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVMGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGQAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALAGDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=156315 AK973_RS02860 WP_003196973.1 679999..681063(+) (comM) [Pseudomonas brassicacearum strain LBUM300]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTGGAGTT
GAGCCGCCAGGGCGATCGATACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCAGCCAACGCTGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGGCAGGCGTTGTCGCGCCTGTTGGCCAAGGCCAAGACGCCCTTGCGTAGCGTGGCGGTG
GCGGTGGCGGGTTCAGCGGTGATCACCAAGACCATCGAGATGGACGCCGGCCTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATCCCCTATCCCCTGGATGAGGTGGCCATCGATTTCGAAGTGATGGGTGTTTCGCCAC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCATTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGTGCTTTTGGCCTGCTCGCCACGCAACTGGC
TGCCTCCCAGGAACGGCTGACTGTGGCGGTCGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAGATCCAGCGCCGCTATGGCCTGACACCC
GAGCAGGCTGGCCAGGCAAAGAAGCAGGGTGGCCTGCCGGACGACTATCTTAGCGAAGTGCTGCAACCTTTTCGCGAGGC
TCTGGTACAGCAGGTCTCGCGGTCCTTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTGGACCACATTCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGCGCCCTGGCCGGTGATGCGCCGGCACTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q7AAJ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475

Multiple sequence alignment