Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   RN80_RS02175 Genome accession   NZ_CP012646
Coordinates   411525..412823 (+) Length   432 a.a.
NCBI ID   WP_045612473.1    Uniprot ID   A0A0F2DEF9
Organism   Streptococcus mitis strain KCOM 1350 (= ChDC B183)     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406525..417823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN80_RS02150 (RN80_02160) rpsB 407330..408109 (+) 780 WP_007556349.1 30S ribosomal protein S2 -
  RN80_RS02155 (RN80_02165) tsf 408188..409228 (+) 1041 WP_060627333.1 translation elongation factor Ts -
  RN80_RS02160 (RN80_02170) cysK 409351..410277 (-) 927 WP_049521100.1 cysteine synthase A -
  RN80_RS02165 (RN80_02175) - 410374..410817 (-) 444 WP_060627334.1 PH domain-containing protein -
  RN80_RS02170 (RN80_02180) - 410833..411468 (-) 636 WP_060627335.1 YigZ family protein -
  RN80_RS02175 (RN80_02185) comFA/cflA 411525..412823 (+) 1299 WP_045612473.1 DEAD/DEAH box helicase Machinery gene
  RN80_RS02180 (RN80_02190) comFC/cflB 412820..413482 (+) 663 WP_045612472.1 ComF family protein Machinery gene
  RN80_RS02185 (RN80_02195) hpf 413562..414110 (+) 549 WP_004255322.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RN80_RS02190 (RN80_02200) - 414246..416219 (+) 1974 WP_000715142.1 DHH family phosphoesterase -
  RN80_RS02195 (RN80_02205) rplI 416216..416668 (+) 453 WP_060627336.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49523.37 Da        Isoelectric Point: 8.2099

>NTDB_id=155993 RN80_RS02175 WP_045612473.1 411525..412823(+) (comFA/cflA) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
MKVNPNYLGRLFTENELTKEERQLAEKLPAMRKEKGKLFCQRCDSAILDEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFAKQDILKWRGQLTPFQEKVSEGLIQAVDKQEPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQDDFACEISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPKLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNHCLEKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLKEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNTSIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=155993 RN80_RS02175 WP_045612473.1 411525..412823(+) (comFA/cflA) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
ATGAAAGTAAATCCAAATTATCTCGGTCGTTTGTTTACTGAGAATGAATTAACAAAAGAGGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTGATAGTGCTATTTTAGATGAATGGTATTTGC
CCATCGGTGCTTATTATTGTAGGGAGTGTTTACTGATGAAGCGAGTCAGGAGTGATCAAGCTTTATACTATTTTCCGCAG
GAGGATTTTGCGAAGCAAGATATTCTCAAATGGCGCGGTCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTAAT
TCAAGCTGTAGACAAGCAGGAACCAACCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TAGCTAAAGTGATCAATGCGGGTGGTGCAGTATGCTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAAGATGATTTTGCTTGCGAGATATCTTTACTACACGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAATCCCAAGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGACTGAGAATCTTTTTAACAGCGACTTCGACT
GATGAGTTAGATAGAAAGGTTCGCATAGGAGAACTAAAACGATTGAGTTTGCCAAGACGATTTCATGGAAATCCGTTGAT
TATTCCTAAACCTGTCTGGTTATCCGATTTTAATCACTGTTTAGAGAAAAATCGGTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAAGATAGATTAGAACAGGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATATTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTTG
TAGTAGAGGCCAATCATCGTCTTTTTACCAAGTCTAGTTTAATACAGATTGGAGGACGTGTCGGTCGGAGTATGGACAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATACTTCAATCAAGAAGGCAATTAAGGAAATTCAGCAGATGAA
TAAGGAAGCGGGTCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F2DEF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

96.991

100

0.97
  comFA/cflA Streptococcus mitis NCTC 12261

95.139

100

0.951
  comFA/cflA Streptococcus pneumoniae Rx1

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae D39

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae R6

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae TIGR4

93.519

100

0.935
  comFA Lactococcus lactis subsp. cremoris KW2

50.117

99.306

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

39.03

100

0.391
  comFA Bacillus subtilis subsp. subtilis str. 168

38.329

94.213

0.361

Multiple sequence alignment