Detailed information NTG0155580

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AN158_RS12775
• Genome accession: NZ_CP012587
• Coordinates: 2754278..2755396 (-)
• Length: 372 a.a.
• NCBI ID: WP_069370772.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain CA-17
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2749278..2760396

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AN158_RS12745 (AN158_12735)	-	2750111..2750959 (+)	849	WP_069370771.1	class II glutamine amidotransferase	-
AN158_RS12750 (AN158_12740)	-	2751208..2751663 (+)	456	WP_000782976.1	bacteriohemerythrin	-
AN158_RS12755 (AN158_12745)	-	2751816..2752883 (+)	1068	WP_005120426.1	hypothetical protein	-
AN158_RS12760 (AN158_12750)	-	2752883..2753206 (+)	324	WP_000442589.1	RnfH family protein	-
AN158_RS12765 (AN158_12755)	-	2753261..2753659 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AN158_RS12770 (AN158_12760)	fur	2753771..2754208 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AN158_RS12775 (AN158_12765)	pilU	2754278..2755396 (-)	1119	WP_069370772.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AN158_RS12780 (AN158_12770)	pilT	2755424..2756461 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AN158_RS12785 (AN158_12775)	-	2756588..2757280 (+)	693	WP_069370773.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41963.15 Da
• Isoelectric Point: 6.5679

>NTDB_id=155580 AN158_RS12775 WP_069370772.1 2754278..2755396(-) (pilU) [Acinetobacter baumannii strain CA-17]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKDHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=155580 AN158_RS12775 WP_069370772.1 2754278..2755396(-) (pilU) [Acinetobacter baumannii strain CA-17]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TTATGAGCGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCCTCAATGATCAGCCATCGTAACCATAACTCTAAGGACCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392