Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A8140_RS14080 Genome accession   NZ_CP015863
Coordinates   2927353..2929077 (-) Length   574 a.a.
NCBI ID   WP_005533777.1    Uniprot ID   -
Organism   Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2922353..2934077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8140_RS14045 (A8140_13390) - 2922628..2923341 (-) 714 WP_005533763.1 PilW family protein -
  A8140_RS14050 (A8140_13395) - 2923341..2923940 (-) 600 WP_005533764.1 type IV pilus modification PilV family protein -
  A8140_RS14055 (A8140_13400) - 2923937..2924485 (-) 549 WP_005533765.1 type II secretion system protein -
  A8140_RS14060 (A8140_13405) - 2924604..2924834 (-) 231 WP_005533767.1 type II secretion system protein -
  A8140_RS14065 (A8140_13410) - 2924907..2925365 (-) 459 WP_005533770.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A8140_RS14070 (A8140_13415) - 2925655..2926116 (-) 462 WP_005533773.1 hypothetical protein -
  A8140_RS14075 (A8140_13420) - 2926121..2927344 (-) 1224 WP_005533776.1 type II secretion system F family protein -
  A8140_RS14080 (A8140_13425) pilB 2927353..2929077 (-) 1725 WP_005533777.1 GspE/PulE family protein Machinery gene
  A8140_RS14085 (A8140_13430) - 2929074..2930171 (-) 1098 WP_033000368.1 tetratricopeptide repeat protein -
  A8140_RS14090 (A8140_13435) - 2930156..2931001 (-) 846 WP_005533779.1 ExeA family protein -
  A8140_RS14095 (A8140_13440) mshL 2931044..2932678 (-) 1635 WP_005533780.1 pilus (MSHA type) biogenesis protein MshL -
  A8140_RS14100 (A8140_13445) - 2932699..2933028 (-) 330 WP_005533781.1 hypothetical protein -
  A8140_RS14105 (A8140_13450) gspM 2933021..2933671 (-) 651 WP_005533782.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63681.94 Da        Isoelectric Point: 5.0834

>NTDB_id=155469 A8140_RS14080 WP_005533777.1 2927353..2929077(-) (pilB) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
MKIQLRKRLGDLLVEEGIVSEDQVQQALSAQHSTGQKLGDALIDLGFISEKQMLDFLSQQLGLPLIDLSRAPVDADAVQI
LPEVHARRLRAMVVARNGGTLRVAMSDPADLFTQESLMNLLGEHDLEFIIAPERQLVESFDRYYRRTKEIASFAEQLQAE
HQDVEGFDYGIDEGDSEEVTVVKLVNSMFEDAVQVGASDIHIEPDDKVLRLRQRVDGVLHETLLNEVNIASALVLRLKLM
AHLDISEKRLPQDGRFNIKVRGQSIDIRMSTLPTQYGESVVMRLLNQSSGLRKLEDSGLPPELLARLRRQLSRPHGMILV
TGPTGSGKTTTLYGALSELNEPGKKIITAEDPVEYRLPRITQVQINSKIDLTFSRVLRTFLRQDPDIILIGEMRDQETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTADVLDESRQQWLAGRFPNQV
GQTFHKGAGCQNCNLTGYRGRVGVFEMLELENEMMDKLRASDAVGFAQVARRSENYKPLLASAMELALQGTVSLDEVMAL
GEGDASGKTDPIFM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=155469 A8140_RS14080 WP_005533777.1 2927353..2929077(-) (pilB) [Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920 strain ATCC 25920 CAIM 519T]
ATGAAAATTCAGTTGAGAAAACGCTTAGGCGACCTGTTGGTTGAAGAGGGTATTGTCTCTGAAGATCAGGTCCAGCAAGC
CCTGAGTGCTCAGCATAGCACCGGACAAAAGTTGGGCGACGCTCTTATCGATCTTGGCTTTATCAGTGAAAAGCAGATGT
TAGACTTTTTGTCTCAGCAACTTGGCTTACCATTAATTGATTTAAGCCGTGCGCCTGTTGATGCTGATGCTGTGCAGATC
TTGCCAGAGGTGCATGCGCGCCGCTTACGTGCCATGGTTGTTGCTCGCAATGGTGGCACGCTGCGCGTTGCTATGAGCGA
TCCTGCTGACCTGTTTACGCAAGAATCATTGATGAACTTGCTCGGTGAGCACGATTTAGAATTTATCATTGCACCTGAAC
GCCAGTTAGTCGAAAGCTTTGACCGCTATTATCGCCGAACCAAAGAAATCGCGTCGTTTGCTGAACAGCTGCAAGCTGAG
CACCAAGATGTTGAAGGCTTTGATTACGGCATTGATGAAGGCGACAGCGAAGAAGTTACCGTTGTAAAACTGGTTAACTC
GATGTTTGAAGATGCTGTTCAAGTTGGTGCATCGGATATTCATATTGAGCCAGACGACAAGGTGCTGCGTTTACGTCAGC
GTGTTGATGGTGTGTTGCATGAAACCCTTCTAAACGAAGTCAACATTGCTTCTGCATTGGTACTACGCCTGAAATTAATG
GCGCATCTCGATATCTCTGAAAAGCGCTTACCTCAAGATGGTCGATTCAATATCAAAGTTCGTGGTCAATCGATCGATAT
TCGTATGTCGACTTTGCCAACGCAATACGGTGAATCAGTGGTAATGCGTTTGCTAAACCAATCTTCTGGTTTGCGTAAGC
TTGAAGATTCCGGTTTACCACCTGAGTTACTTGCTCGTCTTCGTCGTCAGCTTTCTCGTCCACACGGAATGATCTTAGTT
ACTGGTCCAACAGGCTCCGGTAAAACCACCACACTTTATGGTGCGTTGAGTGAATTAAACGAACCGGGCAAGAAGATCAT
CACCGCAGAAGATCCGGTGGAATACCGCTTACCGCGCATTACTCAGGTGCAGATCAACAGTAAAATTGATCTGACTTTCT
CGCGAGTGCTGCGAACCTTCCTACGTCAAGACCCAGACATTATCCTTATTGGTGAGATGCGTGACCAAGAAACGGTTGAA
ATAGGCCTCCGTGCTGCACTAACTGGTCACTTAGTACTAAGTACTTTGCACACTAACGATGCGGTGGACAGTGCGTTGCG
TATGATTGATATGGGCGCGCCTGGTTACTTGGTCGCGAGTGCGGTGCGTGCTGTCGTAGCTCAACGACTTGTTCGTCGTG
TCTGCCCTGACTGTAAAACAGCGGATGTGCTTGATGAGTCTCGTCAGCAGTGGCTTGCGGGTCGCTTCCCTAATCAAGTA
GGGCAAACCTTCCACAAAGGCGCGGGTTGCCAAAACTGTAACCTGACCGGTTATCGCGGCCGAGTTGGTGTGTTTGAGAT
GCTTGAGCTAGAAAATGAAATGATGGACAAGCTACGAGCCAGTGATGCTGTAGGCTTTGCTCAAGTGGCGCGCCGCTCAG
AAAACTATAAGCCTCTGTTGGCTTCTGCTATGGAGCTTGCACTGCAAGGTACCGTGAGTTTGGATGAAGTTATGGCTCTT
GGTGAGGGCGATGCTTCTGGTAAAACCGACCCAATTTTCATGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.681

97.735

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.861

100

0.371
  pilB Legionella pneumophila strain ERS1305867

37.39

98.78

0.369
  pilF Thermus thermophilus HB27

37.679

97.561

0.368
  pilB Acinetobacter baumannii D1279779

36.953

99.477

0.368