Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   A8V31_RS14070 Genome accession   NZ_CP015842
Coordinates   2577515..2578525 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli O157:H7 strain FRIK2533     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2572515..2583525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8V31_RS14050 (A8V31_13365) mepM 2573383..2574705 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  A8V31_RS14055 (A8V31_13370) znuA 2574721..2575653 (-) 933 WP_001303192.1 zinc ABC transporter substrate-binding protein ZnuA -
  A8V31_RS14060 (A8V31_13375) znuC 2575732..2576487 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  A8V31_RS14065 (A8V31_13380) znuB 2576484..2577269 (+) 786 WP_000571476.1 zinc ABC transporter permease subunit ZnuB -
  A8V31_RS14070 (A8V31_13385) ruvB 2577515..2578525 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  A8V31_RS14075 (A8V31_13390) ruvA 2578534..2579145 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  A8V31_RS14080 (A8V31_30370) yobI 2579284..2579349 (-) 66 WP_010723105.1 stress response small protein YobI -
  A8V31_RS14085 (A8V31_13395) yebB 2579420..2580022 (+) 603 WP_001024949.1 YebB family permuted papain-like enzyme -
  A8V31_RS14090 (A8V31_13400) ruvC 2580024..2580545 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  A8V31_RS14095 (A8V31_13405) yebC 2580580..2581320 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A8V31_RS14100 (A8V31_13410) nudB 2581349..2581801 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=155028 A8V31_RS14070 WP_000568519.1 2577515..2578525(-) (ruvB) [Escherichia coli O157:H7 strain FRIK2533]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=155028 A8V31_RS14070 WP_000568519.1 2577515..2578525(-) (ruvB) [Escherichia coli O157:H7 strain FRIK2533]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494