Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   A7319_RS23015 Genome accession   NZ_CP015638
Coordinates   5186792..5187850 (-) Length   352 a.a.
NCBI ID   WP_024077146.1    Uniprot ID   A0A0H5AFF7
Organism   Pseudomonas fluorescens strain L111     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5181792..5192850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7319_RS30030 pal 5182084..5182584 (-) 501 WP_003209827.1 peptidoglycan-associated lipoprotein Pal -
  A7319_RS22990 (A7319_22970) tolB 5182640..5183920 (-) 1281 WP_041161007.1 Tol-Pal system beta propeller repeat protein TolB -
  A7319_RS22995 (A7319_22975) tolA 5183938..5185011 (-) 1074 WP_024077142.1 cell envelope integrity protein TolA -
  A7319_RS23000 (A7319_22980) tolR 5185011..5185463 (-) 453 WP_160384999.1 protein TolR -
  A7319_RS23005 (A7319_22985) tolQ 5185487..5186182 (-) 696 WP_024077143.1 protein TolQ -
  A7319_RS23010 (A7319_22990) ybgC 5186184..5186639 (-) 456 WP_032890914.1 tol-pal system-associated acyl-CoA thioesterase -
  A7319_RS23015 (A7319_22995) ruvB 5186792..5187850 (-) 1059 WP_024077146.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  A7319_RS23020 (A7319_23000) ruvA 5187851..5188462 (-) 612 WP_003175802.1 Holliday junction branch migration protein RuvA -
  A7319_RS23025 (A7319_23005) ruvC 5188530..5189054 (-) 525 WP_024077147.1 crossover junction endodeoxyribonuclease RuvC -
  A7319_RS23030 (A7319_23010) - 5189211..5189957 (-) 747 WP_024077148.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A7319_RS23035 (A7319_23015) aspS 5190093..5191868 (-) 1776 WP_024077149.1 aspartate--tRNA ligase -
  A7319_RS23040 (A7319_23020) - 5191976..5192197 (-) 222 WP_010208103.1 FmdB family zinc ribbon protein -
  A7319_RS23045 (A7319_23025) - 5192367..5192708 (-) 342 WP_041161008.1 ribbon-helix-helix domain-containing protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38983.73 Da        Isoelectric Point: 4.9689

>NTDB_id=154367 A7319_RS23015 WP_024077146.1 5186792..5187850(-) (ruvB) [Pseudomonas fluorescens strain L111]
MIEADRLIAATGPRDREEIQDRAIRPLSLADYIGQPTVREQMELFIQAARGRSESLDHTLIFGPPGLGKTTLANIIAQEM
GVSIKSTSGPVLERPGDLAALLTNLEPHDVLFIDEIHRLSPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLVG
ATTRAGMLTNPLRDRFGIVQRLEFYSTADLATIVSRSAGILGLPLDPEGAFEIARRARGTPRIANRLLRRVRDFAEVRAK
GHITKPVADLALNLLDVDEHGFDHQDRRLLLTMIEKFDGGPVGVDSLAAAISEERHTIEDVLEPYLIQQGYIMRTPRGRV
VTRHAYLHFGLNIPSRLGEMPVVDEFLDAVDD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=154367 A7319_RS23015 WP_024077146.1 5186792..5187850(-) (ruvB) [Pseudomonas fluorescens strain L111]
GTGATTGAAGCTGATCGTCTGATCGCGGCCACGGGCCCCCGTGACCGCGAAGAAATCCAGGACCGTGCCATCCGGCCCCT
GAGCCTGGCCGACTACATCGGCCAGCCTACCGTGCGCGAGCAGATGGAGTTGTTCATCCAGGCCGCGCGCGGGCGCAGCG
AGTCCCTGGATCACACCCTGATCTTTGGCCCGCCGGGGCTGGGTAAAACCACCCTGGCCAACATCATTGCCCAGGAAATG
GGCGTGTCGATCAAGTCGACTTCCGGCCCGGTGCTGGAGCGTCCTGGCGACCTGGCGGCGCTGCTGACCAATCTTGAACC
CCATGACGTATTGTTCATCGACGAAATCCATCGGTTGTCGCCGATTGTCGAGGAAGTGCTGTACCCCGCCATGGAAGACT
TCCAGCTCGACATCATGATCGGCGAAGGCCCCGCGGCCCGATCGATCAAGCTCGACCTGCCGCCGTTCACCCTGGTGGGG
GCGACCACGCGTGCGGGTATGCTGACCAACCCGCTGCGAGACCGTTTCGGTATCGTTCAACGTCTGGAGTTCTATAGCAC
CGCAGACCTGGCGACCATCGTCAGCCGTTCGGCCGGCATTCTCGGCCTGCCCCTGGACCCGGAAGGCGCGTTTGAAATCG
CCCGTCGGGCCCGGGGCACGCCACGTATTGCCAACCGCCTGCTGCGCCGCGTGCGTGATTTTGCCGAGGTGCGGGCCAAG
GGGCATATCACCAAGCCGGTGGCCGACCTGGCCTTGAACCTGCTGGATGTGGACGAGCATGGCTTCGATCACCAGGATCG
ACGTCTACTCTTGACCATGATCGAGAAGTTCGACGGTGGCCCGGTGGGGGTAGACAGCCTGGCCGCGGCCATCAGTGAAG
AGCGCCATACCATTGAAGATGTGCTGGAACCGTACCTGATCCAGCAGGGCTACATCATGCGCACGCCAAGGGGGCGGGTG
GTCACGCGCCATGCCTATTTGCACTTCGGGTTAAACATTCCGTCACGATTGGGTGAGATGCCCGTGGTAGACGAATTCCT
GGATGCAGTGGACGATTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(24-182)

RuvB AAA lid domain (AAA_lid_4)

(185-258)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H5AFF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.606

93.75

0.568
  ruvB Streptococcus pneumoniae TIGR4

58.75

90.909

0.534
  ruvB Streptococcus pneumoniae R6

58.75

90.909

0.534
  ruvB Streptococcus pneumoniae D39

58.75

90.909

0.534
  ruvB Synechocystis sp. PCC 6803

53.959

96.875

0.523
  ruvB Helicobacter pylori 26695

53.894

91.193

0.491

Multiple sequence alignment