Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KS2013_RS01810 Genome accession   NZ_CP012418
Coordinates   368610..369677 (+) Length   355 a.a.
NCBI ID   WP_068988849.1    Uniprot ID   A0A1B3B8G0
Organism   Kangiella sediminilitoris strain KCTC 23892     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 363610..374677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KS2013_RS01800 (KS2013_362) rpmE 365471..365680 (+) 210 WP_068988843.1 50S ribosomal protein L31 -
  KS2013_RS01805 (KS2013_363) - 365751..368318 (-) 2568 WP_228703697.1 penicillin-binding protein 1A -
  KS2013_RS01810 (KS2013_364) pilM 368610..369677 (+) 1068 WP_068988849.1 pilus assembly protein PilM Machinery gene
  KS2013_RS01815 (KS2013_365) - 369677..370279 (+) 603 WP_068988853.1 PilN domain-containing protein -
  KS2013_RS01820 (KS2013_366) - 370279..370896 (+) 618 WP_068988855.1 type 4a pilus biogenesis protein PilO -
  KS2013_RS01825 (KS2013_367) - 370899..371456 (+) 558 WP_068988858.1 pilus assembly protein PilP -
  KS2013_RS01830 (KS2013_368) pilQ 371464..373638 (+) 2175 WP_228703698.1 type IV pilus secretin PilQ -
  KS2013_RS01835 (KS2013_369) aroK 373733..374257 (+) 525 WP_068988862.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38467.74 Da        Isoelectric Point: 4.3775

>NTDB_id=154349 KS2013_RS01810 WP_068988849.1 368610..369677(+) (pilM) [Kangiella sediminilitoris strain KCTC 23892]
MVLGLFKKKQVPVIGIDISSTAVKVLQLGKSGGRFRVDRYAVEPLPQGAVVERDIRDTEAVGNAIKKAVQKAQAKTKDAA
VAVSGSAVITRSIQMEKGLTDQEMEDTIKVEADQYIPYPLEEVNLDFEVLGDSPNDDNMVDVLLAASRSINVDTRIDALE
IAGLTPKIVDIEAYAIERAYQLTKKKTIEHMEDSVVAIVDIGATMTSLNVLQNGQVIYNREQLFGGNQLTEEIQSRYGLS
YQEAGLAKKQGSLPDDYESEVLMPFAEIITQQVGRALQFFFSASEFSSVNEVILAGGCASIDGLDGMIQEHLGTPTRVAN
PFADMSISSHVNTQALSADAPAMMISCGLALRSFD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=154349 KS2013_RS01810 WP_068988849.1 368610..369677(+) (pilM) [Kangiella sediminilitoris strain KCTC 23892]
ATGGTTCTGGGGTTATTCAAAAAGAAACAAGTACCAGTAATCGGTATTGATATCAGCTCGACCGCTGTCAAAGTCCTGCA
GTTAGGAAAATCTGGTGGTCGTTTCCGCGTTGATAGATATGCCGTCGAACCATTACCGCAAGGTGCTGTTGTAGAGCGTG
ATATTCGTGATACCGAGGCCGTGGGTAATGCAATTAAAAAAGCGGTGCAGAAAGCGCAGGCTAAAACCAAGGATGCAGCA
GTCGCTGTATCTGGCTCGGCGGTCATTACACGCTCAATCCAAATGGAGAAAGGCCTTACTGACCAAGAGATGGAAGATAC
CATTAAGGTAGAAGCCGATCAGTACATCCCTTATCCGTTAGAAGAGGTTAACCTGGACTTTGAGGTTCTAGGTGATTCTC
CGAATGATGACAATATGGTGGATGTATTATTAGCTGCTTCACGAAGTATCAATGTTGATACGCGAATTGATGCGCTGGAA
ATAGCGGGTCTGACACCAAAAATTGTAGATATTGAGGCCTACGCCATAGAGCGAGCGTATCAATTGACCAAGAAAAAGAC
CATAGAACATATGGAAGACTCAGTAGTCGCCATCGTTGATATCGGTGCAACAATGACGAGTCTTAACGTACTGCAAAATG
GTCAGGTTATTTACAACCGTGAACAATTATTCGGTGGGAATCAGTTAACAGAAGAAATTCAAAGTCGATACGGTCTGTCC
TATCAGGAAGCTGGTCTTGCAAAGAAGCAGGGGAGCTTACCAGATGATTATGAATCAGAAGTGTTAATGCCATTTGCAGA
AATCATCACACAGCAGGTTGGTCGAGCACTTCAGTTCTTCTTCTCTGCCAGTGAGTTTAGCTCCGTTAATGAAGTGATTC
TCGCGGGTGGCTGTGCTTCGATTGATGGCCTGGATGGTATGATTCAGGAACATTTAGGTACTCCAACTCGAGTCGCAAAT
CCTTTCGCGGATATGTCAATATCATCACATGTGAATACACAAGCATTAAGTGCTGATGCGCCAGCAATGATGATAAGCTG
TGGACTAGCATTAAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(15-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B3B8G0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50

99.718

0.499
  comM Acinetobacter nosocomialis M2

49.718

99.718

0.496
  comM Acinetobacter baylyi ADP1

47.175

99.718

0.47
  pilM Legionella pneumophila strain ERS1305867

46.893

99.718

0.468

Multiple sequence alignment