Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DE8669_RS04290 Genome accession   NZ_CP012391
Coordinates   822085..823464 (+) Length   459 a.a.
NCBI ID   WP_002223905.1    Uniprot ID   A0A0Y5CWB3
Organism   Neisseria meningitidis strain DE8669     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 817085..828464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DE8669_RS04270 (DE8669_0805) - 817433..818194 (+) 762 WP_002223909.1 uroporphyrinogen-III synthase -
  DE8669_RS04275 (DE8669_0806) - 818209..819543 (+) 1335 WP_002258524.1 uroporphyrinogen-III C-methyltransferase -
  DE8669_RS04280 (DE8669_0807) - 819540..820757 (+) 1218 WP_014581154.1 heme biosynthesis protein HemY -
  DE8669_RS04285 (DE8669_0808) hemE 820832..821896 (+) 1065 WP_002219525.1 uroporphyrinogen decarboxylase -
  DE8669_RS04290 (DE8669_0809) radA 822085..823464 (+) 1380 WP_002223905.1 DNA repair protein RadA Machinery gene
  DE8669_RS04295 (DE8669_0810) - 823535..824014 (-) 480 WP_002219523.1 DUF302 domain-containing protein -
  DE8669_RS04300 (DE8669_0811) - 824256..824615 (-) 360 WP_002219522.1 rhodanese-like domain-containing protein -
  DE8669_RS04305 (DE8669_0812) recB 824654..828268 (-) 3615 WP_014581153.1 exodeoxyribonuclease V subunit beta Machinery gene

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49575.07 Da        Isoelectric Point: 7.0604

>NTDB_id=154070 DE8669_RS04290 WP_002223905.1 822085..823464(+) (radA) [Neisseria meningitidis strain DE8669]
MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPKNARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVSNPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFT
PKRLTVGLEQNRLAMLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAFGEIGLSGE
VRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEAIDICREDRD

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=154070 DE8669_RS04290 WP_002223905.1 822085..823464(+) (radA) [Neisseria meningitidis strain DE8669]
ATGGCAAAAACGCTTAAAACCCTTTACCAATGCACCGAATGCGGCGGCACTTCCCCGAAATGGCAGGGCAAATGCCCGCA
TTGCGGCGAGTGGAACACACTTCAGGAAAGCTTGGCCGCGCCCGAGCCGAAAAACGCCCGCTTCCAATCTTGGGCGGCGG
ATACCTCGACCGTCCAATCCCTCTCCTCCGTGACCGCCACCGAAGTGCCGCGCAATCCGACCGGCATGGGCGAACTCGAC
CGCGTATTGGGCGGCGGTTTGGTCGATGGTGCGGTCATCCTGCTCGGCGGCGACCCCGGCATCGGCAAATCTACGCTGCT
GCTGCAAACCATCGCCAAAATGGCGCAAAGCCGTAAAGTGCTATACGTTTCCGGCGAAGAATCCGCCCAACAGGTCGCCC
TGCGCGCGCAGCGTTTGGAACTGCCCACCGAAGGCGTGAACCTGCTTGCCGAAATCCGCATGGAAGCGATTCAAGCAGCG
TTGAAACAGCATCAGCCCGAAGTCGTGGTTATCGATTCCATCCAAACCATGTATTCCGACCAAATCACTTCCGCCCCCGG
TTCTGTGTCGCAGGTGCGCGAGTGTGCCGCCCAACTGACGCGCATGGCGAAACAGATGGGCATCGCCATGATACTGGTCG
GACACGTGACCAAAGACGGCGCGATTGCCGGCCCGCGCGTGCTGGAACACATGGTTGATACCGTGCTGTATTTCGAGGGC
GACCAACATTCCAACTACCGCATGATACGCGCCATCAAAAACCGCTTCGGCGCGGCAAACGAACTGGGCGTGTTCGCGAT
GACGGAAAACGGTTTGAAAGGTGTGTCCAACCCGTCCGCCATCTTCCTTGCCAGCTACCGCGACGACACGCCCGGTTCGT
GCGTTTTGGTTACGCAGGAAGGCAGCCGACCGCTTTTGGTTGAAATTCAGGCATTGGTCGATGACGCGCACGGATTCACC
CCCAAACGCCTTACCGTCGGACTGGAACAAAACCGCCTCGCGATGCTGCTCGCCGTGTTAAACCGACACGGCGGCATCGC
CTGTTTCGATCAGGATGTGTTCCTCAACGCCGTCGGTGGCGTGAAAATCGGCGAACCGGCGGCGGATTTGGCAGTCATCC
TCGCGATGCTTTCCAGCTTCCGCAACCGCCCGCTGCCCGAAAAAATGGTTGCATTTGGAGAAATCGGTTTGAGCGGTGAA
GTCCGCCCCGTCGCGCGCGGACAAGAGCGGCTCAAAGAAGCGGAAAAACTCGGCTTCAAACGCGCCATCGTCCCCAAAGC
CAATATGCCGCGCAACGCCAAAGAGTTTCCGAACCTGAAAATCTACGGCGTTTCGAGTTTGCAGGAAGCCATTGATATTT
GTCGGGAAGATAGGGATTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(366-428)

AAA domain (AAA_25)

(81-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Y5CWB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.33

99.129

0.499
  radA Streptococcus pneumoniae Rx1

47.566

98.475

0.468
  radA Streptococcus pneumoniae D39

47.566

98.475

0.468
  radA Streptococcus pneumoniae R6

47.566

98.475

0.468
  radA Streptococcus pneumoniae TIGR4

47.566

98.475

0.468
  radA Streptococcus mitis SK321

47.45

98.257

0.466
  radA Streptococcus mitis NCTC 12261

47.45

98.257

0.466

Multiple sequence alignment