Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   A4W82_RS03735 Genome accession   NZ_CP015487
Coordinates   733872..735755 (+) Length   627 a.a.
NCBI ID   WP_025015867.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain TMW 1.46     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 728872..740755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W82_RS03720 (A4W82_03685) fmt 730818..731774 (+) 957 WP_076647396.1 methionyl-tRNA formyltransferase -
  A4W82_RS03725 (A4W82_03690) rsmB 731764..733107 (+) 1344 WP_251925853.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  A4W82_RS03730 (A4W82_03695) - 733129..733875 (+) 747 WP_025015868.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  A4W82_RS03735 (A4W82_03700) pknB 733872..735755 (+) 1884 WP_025015867.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  A4W82_RS03740 (A4W82_03705) rsgA 735855..736754 (+) 900 WP_011374402.1 ribosome small subunit-dependent GTPase A -
  A4W82_RS03745 (A4W82_03710) rpe 736778..737440 (+) 663 WP_076647402.1 ribulose-phosphate 3-epimerase -
  A4W82_RS03750 (A4W82_03715) - 737427..738077 (+) 651 WP_016264867.1 thiamine diphosphokinase -
  A4W82_RS03755 (A4W82_03720) rpmB 738427..738612 (-) 186 WP_004265581.1 50S ribosomal protein L28 -
  A4W82_RS03760 (A4W82_03725) - 738915..739277 (+) 363 WP_016264868.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 68228.85 Da        Isoelectric Point: 5.9848

>NTDB_id=153934 A4W82_RS03735 WP_025015867.1 733872..735755(+) (pknB) [Latilactobacillus sakei strain TMW 1.46]
MMETGYAVAGRYKIIRPIGEGGMANVYLAQDLILDRQVAVKVLRLDLRNDPDTVRRFTREALATTELNHPNIVSIYDVGE
ENSMQYIIMEYIKGTDLKKYIVEHFPIPYQRVIDIMTQILSAVQNAHAHNIIHRDLKPQNILVDEDGNVKISDFGIAIAL
SETAMTQTNTLLGSVHYLSPEQARGSMATKRSDIYSLGIVLYELLTGMVPFEGESAVSIAIKHFQDEVPPVRNYDPRIPQ
ALENVVLKATAKDPDERYSGVDAMMADLATSLSASRAHEPKFVPSKADDLSETKVIPALDPEGITQTNNDEEQVKEAEPT
QKKVKKPAKSKRKWWLLGGGVVLLLLLATVIAFAVQGSEVTVPDLTDMTQQNAESALTDKKLKVGTVQKTTSQKYSKGHV
IRTTPKAGLSVKSRSTVNLIVSSGRQKYKIKDYTNQAYSDVRDALKDKGLKVKRKYQSSSEVSPGLIIDQSIAAGKRVVP
SKTTITLTVSSGKPGFALHNLSNYTKQGIQDYAAQNGLTVVFSEEYSSSISEGMVISQSPASGTMVQEGDSVSVILSKGE
QPTEHHSIVTSSSNSVIESSAPESSQSSESSASESESESESESESESASESISSSASQSSQSSVQSK

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=153934 A4W82_RS03735 WP_025015867.1 733872..735755(+) (pknB) [Latilactobacillus sakei strain TMW 1.46]
ATGATGGAAACAGGGTACGCTGTAGCGGGGCGTTATAAGATTATTCGCCCCATCGGTGAAGGTGGCATGGCTAATGTTTA
TTTGGCGCAGGATCTAATTTTAGATCGGCAAGTGGCAGTTAAAGTTTTACGATTAGATCTCCGAAATGATCCGGATACAG
TCCGACGGTTTACGCGAGAAGCGTTGGCAACGACTGAATTAAATCATCCGAATATTGTTAGTATTTACGATGTTGGTGAA
GAAAACAGTATGCAATACATCATTATGGAATATATTAAGGGCACCGATCTTAAGAAATATATCGTGGAACACTTTCCAAT
TCCTTATCAACGCGTAATTGATATTATGACGCAGATTTTATCTGCGGTTCAAAACGCACATGCGCATAATATTATTCATC
GGGATTTAAAACCACAAAATATTTTGGTTGATGAAGACGGCAACGTTAAGATTAGTGACTTTGGGATTGCGATTGCGCTC
AGCGAAACGGCGATGACTCAGACCAATACGTTGTTGGGGTCTGTTCATTATCTCTCACCGGAACAGGCAAGAGGTTCGAT
GGCGACGAAACGGTCTGATATTTACTCGTTAGGAATTGTGCTTTATGAGTTATTGACGGGGATGGTGCCTTTTGAAGGCG
AGTCCGCTGTTTCAATTGCGATTAAGCATTTCCAAGATGAAGTCCCACCAGTACGCAACTATGATCCACGTATTCCGCAA
GCCTTGGAAAATGTCGTTTTAAAAGCGACTGCCAAGGATCCGGATGAACGCTATAGTGGTGTTGATGCAATGATGGCTGA
CTTAGCAACTTCTTTATCAGCTAGTCGTGCGCACGAACCTAAATTTGTGCCATCAAAAGCGGATGATTTGAGTGAGACTA
AGGTGATTCCCGCACTAGATCCAGAAGGCATTACGCAAACCAACAATGATGAGGAACAAGTTAAAGAAGCGGAACCAACT
CAGAAAAAGGTTAAAAAGCCAGCTAAGTCTAAACGTAAGTGGTGGCTACTAGGTGGCGGTGTTGTTTTATTGCTGTTATT
AGCAACGGTGATTGCTTTTGCAGTTCAGGGGAGCGAAGTAACCGTCCCTGATTTAACGGATATGACACAACAAAATGCGG
AATCTGCTTTGACCGATAAAAAACTGAAGGTCGGCACGGTTCAAAAAACAACGAGTCAGAAATACAGTAAGGGCCATGTT
ATTCGAACAACGCCTAAAGCAGGCCTGAGTGTAAAATCAAGAAGTACGGTTAATTTAATTGTCAGTTCAGGACGCCAAAA
GTATAAGATTAAAGACTATACGAACCAAGCATATAGTGATGTTCGAGATGCTTTGAAGGATAAAGGCTTGAAAGTTAAAC
GGAAATACCAAAGTTCATCTGAAGTGAGTCCTGGGTTGATTATCGATCAAAGTATTGCGGCTGGCAAAAGGGTCGTTCCA
AGTAAAACGACGATTACCTTAACAGTCAGCAGCGGCAAACCTGGTTTTGCATTGCATAATCTAAGTAACTATACAAAGCA
AGGGATTCAAGACTATGCTGCACAAAATGGTTTAACGGTCGTATTCTCTGAAGAATATTCATCAAGTATTTCTGAAGGAA
TGGTAATCAGTCAATCGCCTGCAAGTGGTACAATGGTTCAAGAAGGTGATAGCGTAAGCGTTATCCTTTCAAAAGGCGAG
CAACCAACTGAACATCATTCTATTGTGACGTCGAGTTCAAATTCAGTTATCGAGTCGAGTGCGCCCGAATCAAGTCAATC
GAGTGAAAGTAGCGCGAGTGAGTCAGAGAGCGAATCAGAGAGTGAATCGGAAAGCGAATCAGCAAGTGAATCGATCAGTA
GTTCAGCTAGTCAAAGTAGTCAATCGAGCGTTCAATCAAAGTAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(12-263)

PASTA domain (PASTA)

(429-490)

PASTA domain (PASTA)

(360-422)

PASTA domain (PASTA)

(500-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

41.88

93.301

0.391
  stkP Streptococcus pneumoniae TIGR4

40.304

94.577

0.381
  stkP Streptococcus pneumoniae D39

40.135

94.577

0.38
  stkP Streptococcus pneumoniae R6

40.135

94.577

0.38
  stkP Streptococcus pneumoniae Rx1

39.966

94.577

0.378