Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AKO66_RS14725 Genome accession   NZ_CP012330
Coordinates   2861070..2864774 (-) Length   1234 a.a.
NCBI ID   WP_057079726.1    Uniprot ID   -
Organism   Bacillus altitudinis strain NJ-V     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2856070..2869774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKO66_RS14710 (AKO66_14655) - 2856160..2856459 (-) 300 WP_057079724.1 hypothetical protein -
  AKO66_RS14715 (AKO66_14660) sbcC 2856477..2859872 (-) 3396 WP_057079725.1 exonuclease subunit SbcC -
  AKO66_RS14720 (AKO66_14665) sbcD 2859869..2861044 (-) 1176 WP_025207109.1 exonuclease subunit SbcD -
  AKO66_RS14725 (AKO66_14670) addA 2861070..2864774 (-) 3705 WP_057079726.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AKO66_RS14730 (AKO66_14675) addB 2864752..2868261 (-) 3510 WP_057079727.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AKO66_RS14735 (AKO66_14680) - 2868428..2868874 (+) 447 WP_007499129.1 ferritin-like domain-containing protein -
  AKO66_RS14740 (AKO66_14685) - 2869067..2869477 (-) 411 WP_008348626.1 DoxX family protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142715.70 Da        Isoelectric Point: 5.0967

>NTDB_id=153522 AKO66_RS14725 WP_057079726.1 2861070..2864774(-) (addA) [Bacillus altitudinis strain NJ-V]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPEQWMRAFLSLYDVDSQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LTLSKEPGGPAPRAENFIDDLEQVNELIRHQDDFGTLYELLPNINFKRLKTCKGDDYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPDQHLKSLAEMKPVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGKHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKADSNETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTSEMLLVHLDQHDTENDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCRKLERFFLQLRKWRDLSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGDDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLSHPSSFSITFHQAFDL
LKEDMLLEKQKQDEVVQALMEGVPVEGYGDYEEEVKERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSVRKAR
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIPLPSNEPYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTAIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVTGRFSGGLEEAEAMLKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=153522 AKO66_RS14725 WP_057079726.1 2861070..2864774(-) (addA) [Bacillus altitudinis strain NJ-V]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGCTCATTCGAAAAATGACTCGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGTTCCCTTCATATGCGGAGACAACTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGTTTGCAAGTTCTCCGTACTTTTTATTATGAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGCTGGAAAGCCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGGTCTCATCCAGCGCCCGAACAATGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATTCACAGACAAAGGTAG
AAGAATTACCCTTTTATCCATATATCAAAGAGGATCTTTCTCTCGTTTTAAGAAGCTGTCAGGAACTGCTGGAACAAGCG
CTCACTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGGAACACTTTATGAGCTTTTGCCAAACATCAATTTTAAAAGGTTAAAGACTTGCAAAG
GGGATGACTATGACCCAGCTTTGTTAGAAAAAGCAACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGACCAGCATTTAAAAAGCTTGGCTGAAATGAAGCCAGTTGTAGAAACACTTGTAGA
GCTTGTGATTCAATATGGAGAGCGTTTCGAGAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCTGATCTAGAACATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCTAAGTACTATCAACAGCAA
TTTGAAGAAGTACTCGTGGATGAATACCAGGATACAAACCTTGTCCAAGAAACGATTTTAAAGCTCGTGTCTAAAGGAAA
ACATCCTGCCGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTTAAAGCAGACAGCAATGAAACAGGTAAAAGAATTGATCTAAATAAAAACTTCCGC
AGCCGGGCTGATGTTTTAGACAGTACCAACTTTCTGTTCAAACAGCTGATGGGAGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGACACGACGTCAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAACGATGAAGAACGAGAAGAGCTAGAGACGGTACAATTTGAGGCGAGAGTGATTGCGAAGAAA
ATAAAAGAATTGGTAGAACAGCCTTTTCAAGTGTACGATGCAAAACAGCAGCTGACCCGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGCTCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCCTCAGGTTATTTTGAAGCTACAGAAGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTCGCTGCTGTTTTAAGGTCACCTCTTGTTCATTTAGACGAAAATGAGATGGCACTGATTAGAACCAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACGTGCAGAAAGCTGG
AGCGTTTCTTCCTTCAATTACGCAAATGGCGTGATTTATCTATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGCAAGCAAAGACAGGCAAACCTTCGGGCTTTATATGA
TCGAGCAAAACAGTATGAAAAGGCTGCTTTTAGAGGGCTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTGTTTACTGCAGGACTTGGCAGGAACTTTAATATGATGGATCTAAATCAGTCCTACTTGCTCGA
TAAAGAACTAGGTTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAACTATTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCGAAAGAAAAATTA
TTTTTAGTCGGTTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCCGCAAACGGAGACGACTGGCTCCT
CCCTGATTTTGAACGTTATCAAGCGAGAACATACTTAGATTTTATTGGCCCTGCTCTTATCCGTCATCAAGCGATGTCAT
CTCTTTTAGAAGAATCGAGAGAAGTCGTTCTCTCTCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTTCGATCTT
CTAAAAGAAGACATGTTACTTGAAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGGGTGCCTGTTGAAGG
ATATGGCGATTACGAGGAGGAAGTAAAAGAAAGGCTTTCATGGCGATATCCATATTTAGCGGCCTCCCAAGTAGGGACAA
AGCAGTCAGTTTCTGAGATCAAAAGAATGAAAGAAATACAGGATGAATACAGTGTGCCTTCTTCCGTTCGAAAAGCTCGT
GCGACATTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCCTCTTCCTTCAAATGAACCATACGATGAATCTCGTGTAGAAGAGCTGCTCCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATCCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGCTATTGGTCAA
AAACTGCGTAAAGCGGACTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTGCTCATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGCAAGCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGATTAGAGGAAGCTGAAGCGATGTTAAAAAAACGTTATGAA
ACGCAAATATCGTTGTATACAGCAGCGGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATCCTTTATTTCTTTGA
TGGAAATCTAGAAATTTCTTTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.774

100

0.669

Multiple sequence alignment