Detailed information NTG0150119

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACX61_RS14550
• Genome accession: NZ_CP012006
• Coordinates: 3046511..3047629 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347035.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain Ab04-mff
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3041511..3052629

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACX61_RS14540 (ACX61_14540)	-	3044627..3045319 (-)	693	WP_001108510.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACX61_RS14545 (ACX61_14545)	pilT	3045446..3046483 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACX61_RS14550 (ACX61_14550)	pilU	3046511..3047629 (+)	1119	WP_000347035.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACX61_RS14555 (ACX61_14555)	fur	3047699..3048136 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACX61_RS14560 (ACX61_14560)	-	3048248..3048646 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACX61_RS14565 (ACX61_14565)	-	3048701..3049024 (-)	324	WP_000442585.1	RnfH family protein	-
ACX61_RS14570 (ACX61_14570)	-	3049024..3050091 (-)	1068	WP_005120426.1	hypothetical protein	-
ACX61_RS14575 (ACX61_14575)	-	3050244..3050699 (-)	456	WP_034129532.1	bacteriohemerythrin	-
ACX61_RS14580 (ACX61_14580)	-	3050952..3051800 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41821.98 Da
• Isoelectric Point: 6.5675

>NTDB_id=150119 ACX61_RS14550 WP_000347035.1 3046511..3047629(+) (pilU) [Acinetobacter baumannii strain Ab04-mff]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDSQ

Nucleotide

• Length: 1119 bp

>NTDB_id=150119 ACX61_RS14550 WP_000347035.1 3046511..3047629(+) (pilU) [Acinetobacter baumannii strain Ab04-mff]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACAGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.817	100	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39