Detailed information NTG0150072

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACX60_RS13900
• Genome accession: NZ_CP012004
• Coordinates: 2935301..2936419 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain ATCC 17978-mff
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2930301..2941419

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACX60_RS13890 (ACX60_13895)	-	2933417..2934109 (-)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACX60_RS13895 (ACX60_13900)	pilT	2934236..2935273 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
ACX60_RS13900 (ACX60_13905)	pilU	2935301..2936419 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACX60_RS13905 (ACX60_13910)	fur	2936489..2936926 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ACX60_RS13910 (ACX60_13915)	-	2937038..2937436 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ACX60_RS13915 (ACX60_13920)	-	2937491..2937814 (-)	324	WP_000442590.1	RnfH family protein	-
ACX60_RS13920 (ACX60_13925)	-	2937814..2938881 (-)	1068	WP_004748097.1	hypothetical protein	-
ACX60_RS13925 (ACX60_13930)	-	2939034..2939489 (-)	456	WP_000782976.1	bacteriohemerythrin	-
ACX60_RS13930 (ACX60_13935)	-	2939742..2940590 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=150072 ACX60_RS13900 WP_000347034.1 2935301..2936419(+) (pilU) [Acinetobacter baumannii strain ATCC 17978-mff]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=150072 ACX60_RS13900 WP_000347034.1 2935301..2936419(+) (pilU) [Acinetobacter baumannii strain ATCC 17978-mff]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39