Detailed information NTG0150020

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PA50071_RS26315
• Genome accession: NZ_CP012001
• Coordinates: 5728825..5729889 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5723825..5734889

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA50071_RS26290 (PA50071_26300)	aroK	5724360..5724878 (-)	519	WP_003095833.1	shikimate kinase AroK	-
PA50071_RS26295 (PA50071_26305)	pilQ	5724890..5727034 (-)	2145	WP_012614629.1	type 4a pilus secretin PilQ	Machinery gene
PA50071_RS26300 (PA50071_26310)	pilP	5727088..5727612 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
PA50071_RS26305 (PA50071_26315)	pilO	5727609..5728232 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	-
PA50071_RS26310 (PA50071_26320)	pilN	5728229..5728825 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
PA50071_RS26315 (PA50071_26325)	pilM	5728825..5729889 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
PA50071_RS26320 (PA50071_26330)	-	5730074..5732545 (+)	2472	WP_003103276.1	penicillin-binding protein 1A	-
PA50071_RS26325 (PA50071_26335)	-	5732650..5733918 (-)	1269	WP_048521721.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=150020 PA50071_RS26315 WP_003110888.1 5728825..5729889(-) (pilM) [Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=150020 PA50071_RS26315 WP_003110888.1 5728825..5729889(-) (pilM) [Pseudomonas aeruginosa DSM 50071 = NBRC 12689 strain DSM 50071]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACGCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466