Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ECTOBSL9_RS02715 Genome accession   NZ_CP011994
Coordinates   541442..542503 (+) Length   353 a.a.
NCBI ID   WP_063463768.1    Uniprot ID   -
Organism   Ectothiorhodospira sp. BSL-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536442..547503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECTOBSL9_RS02700 (ECTOBSL9_0542) hemE 536978..538042 (+) 1065 WP_063463765.1 uroporphyrinogen decarboxylase -
  ECTOBSL9_RS02705 (ECTOBSL9_0543) - 538120..538716 (-) 597 WP_063463766.1 hypothetical protein -
  ECTOBSL9_RS02710 (ECTOBSL9_0544) - 538811..541255 (-) 2445 WP_063463767.1 penicillin-binding protein 1A -
  ECTOBSL9_RS02715 (ECTOBSL9_0545) pilM 541442..542503 (+) 1062 WP_063463768.1 pilus assembly protein PilM Machinery gene
  ECTOBSL9_RS02720 (ECTOBSL9_0546) - 542503..543075 (+) 573 WP_063463769.1 PilN domain-containing protein -
  ECTOBSL9_RS02725 (ECTOBSL9_0547) - 543072..543683 (+) 612 WP_063463770.1 type 4a pilus biogenesis protein PilO -
  ECTOBSL9_RS02730 (ECTOBSL9_0548) - 543769..544241 (+) 473 Protein_538 pilus assembly protein PilP -
  ECTOBSL9_RS02735 (ECTOBSL9_0549) pilQ 544261..546384 (+) 2124 WP_063463771.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38275.82 Da        Isoelectric Point: 4.6120

>NTDB_id=149964 ECTOBSL9_RS02715 WP_063463768.1 541442..542503(+) (pilM) [Ectothiorhodospira sp. BSL-9]
MLGLGRKTPALIGIDISSTSVKLVELAGSPGKLRVEAYAVEPLPGSAVVEKNIQEVEAVGEAIRKAYKRTGSKTRLCALA
VPSSAVITKTINMPANLKPDEMEGQIQIEADQYIPYALEEVNLDFEVIGPTPKNPETVEVLLSASRSENVDMRVTAAEIG
GLTPKVMDVEAYAIEHSYPLVTEQIPDLDTELPVAVVDVGATMTSINIFLNDRLIYTREQSFGGKQLTEEIMRRYGLSYE
EAGQAKKEGGLPDNYVPEVLEPFKETMAQQVNRFLQFFFAASPHDRVSHILLAGGCAAIPGVDEVIEGRVNTPTSIANPF
GRMALHTRINPQRLGNDAPALMIAAGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=149964 ECTOBSL9_RS02715 WP_063463768.1 541442..542503(+) (pilM) [Ectothiorhodospira sp. BSL-9]
GTGTTAGGACTCGGCCGCAAAACCCCCGCGCTGATAGGCATCGACATCAGCTCCACCTCCGTGAAACTGGTGGAACTGGC
GGGCTCGCCCGGCAAGCTCCGGGTGGAAGCCTACGCCGTGGAACCACTCCCCGGCAGCGCGGTGGTGGAAAAGAACATAC
AGGAAGTGGAAGCCGTAGGCGAAGCCATCCGCAAGGCCTACAAGCGAACCGGCAGCAAGACGCGCCTTTGCGCGCTGGCC
GTGCCGTCATCCGCCGTGATCACCAAAACCATCAACATGCCGGCCAACCTCAAGCCGGATGAGATGGAAGGCCAGATCCA
GATCGAGGCCGACCAGTACATCCCCTACGCCCTGGAAGAAGTGAACCTGGATTTCGAGGTCATCGGCCCCACCCCCAAGA
ACCCCGAAACCGTCGAGGTGCTGCTCTCCGCCTCCCGCAGCGAAAACGTGGACATGCGTGTCACTGCCGCCGAGATCGGT
GGCCTCACCCCCAAGGTGATGGACGTGGAAGCCTACGCCATCGAGCACAGCTACCCGCTGGTCACCGAGCAGATCCCCGA
CCTGGACACCGAACTCCCCGTGGCCGTGGTGGATGTGGGCGCCACCATGACATCCATCAACATCTTCCTCAATGACAGGC
TCATCTACACCCGTGAGCAGTCCTTCGGCGGCAAGCAACTCACCGAAGAGATCATGCGCCGCTACGGCCTCTCCTACGAA
GAGGCCGGCCAGGCCAAGAAGGAAGGCGGCCTGCCCGACAACTACGTACCCGAGGTCCTGGAACCCTTCAAGGAAACCAT
GGCCCAGCAGGTGAACCGCTTCCTGCAGTTCTTCTTCGCCGCCAGCCCCCATGATCGGGTCTCGCACATCCTGCTGGCCG
GTGGCTGTGCCGCCATCCCCGGGGTGGACGAAGTCATCGAAGGCCGGGTGAACACCCCCACCAGCATTGCCAACCCCTTC
GGACGCATGGCGCTGCACACCCGCATCAACCCGCAACGCCTGGGCAACGACGCCCCCGCCCTCATGATCGCCGCCGGGCT
CGCCTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

49.859

100

0.501
  comM Acinetobacter nosocomialis M2

49.859

100

0.501
  comM Acinetobacter baylyi ADP1

46.479

100

0.467
  pilM Legionella pneumophila strain ERS1305867

43.503

100

0.436

Multiple sequence alignment