Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AS588_RS02795 Genome accession   NZ_CP014700
Coordinates   567604..568608 (+) Length   334 a.a.
NCBI ID   WP_003152685.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain S499     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 562604..573608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS588_RS02765 (AS588_02765) nadA 562782..563885 (+) 1104 WP_003152673.1 quinolinate synthase NadA -
  AS588_RS02770 (AS588_02770) safA 564012..565217 (+) 1206 WP_014305504.1 SafA/ExsA family spore coat assembly protein -
  AS588_RS02775 (AS588_02775) - 565365..565958 (+) 594 WP_003152677.1 YhcN/YlaJ family sporulation lipoprotein -
  AS588_RS20480 - 566092..566211 (+) 120 WP_024085651.1 hypothetical protein -
  AS588_RS02785 (AS588_02785) - 566344..566853 (+) 510 WP_012118093.1 intercompartmental signaling factor BofC -
  AS588_RS02790 (AS588_02790) ruvA 566988..567593 (+) 606 WP_003152683.1 Holliday junction branch migration protein RuvA Machinery gene
  AS588_RS02795 (AS588_02795) ruvB 567604..568608 (+) 1005 WP_003152685.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AS588_RS02800 (AS588_02800) - 568601..568801 (+) 201 WP_015387898.1 DUF2905 domain-containing protein -
  AS588_RS02805 (AS588_02805) queA 568827..569855 (+) 1029 WP_003152692.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  AS588_RS02810 (AS588_02810) tgt 569883..571028 (+) 1146 WP_014305500.1 tRNA guanosine(34) transglycosylase Tgt -
  AS588_RS02815 (AS588_02815) yajC 571060..571320 (+) 261 WP_003152695.1 preprotein translocase subunit YajC -
  AS588_RS02820 (AS588_02820) - 571372..571758 (-) 387 WP_003152697.1 TIGR04086 family membrane protein -
  AS588_RS02825 (AS588_02825) - 571925..572581 (+) 657 WP_003152699.1 DUF421 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37225.52 Da        Isoelectric Point: 4.7767

>NTDB_id=149096 AS588_RS02795 WP_003152685.1 567604..568608(+) (ruvB) [Bacillus amyloliquefaciens strain S499]
MDERLVSSEADSHESIIEQSLRPQNLAQYIGQKKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
MRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLNRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVLSRLEYYTQEELTDIVSRTADVFEVDIEEAPALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEEIAHDALERLQVDALGLDHIDHKLLMGMIEKFSGGPVGLDTISATIGEEPHTIEDVYEPYLLQIGFLQRTPRGRVVTP
AVYEHFRLEAPARD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=149096 AS588_RS02795 WP_003152685.1 567604..568608(+) (ruvB) [Bacillus amyloliquefaciens strain S499]
ATGGATGAACGGCTCGTTTCAAGTGAAGCAGACAGCCATGAATCAATAATCGAGCAAAGCCTCAGGCCGCAGAATCTCGC
CCAGTATATCGGCCAAAAGAAGGTGAAAGAAAATCTGCGCGTGTTTATCGATGCGGCGAAAATGAGGCAGGAAACGCTGG
ACCACGTTCTTTTATATGGGCCTCCGGGTCTCGGGAAAACAACGCTTGCGTCTATCGTGGCCAATGAGATGGGGGTGGAG
ATGCGGACGACGTCAGGTCCCGCCATCGAAAGACCGGGAGATTTGGCAGCCATTTTGACGGCGCTCGAACCAGGGGATGT
GCTGTTTATTGACGAAATTCACAGGCTGAACCGCTCAATTGAGGAAGTGCTGTACCCGGCGATGGAGGATTTCTGCCTTG
ATATCGTCATTGGAAAAGGCCCGTCCGCCCGCTCCGTCCGTCTCGATCTTCCCCCTTTTACGCTTGTAGGAGCGACGACA
AGGGTCGGATTATTGACCGCACCGCTCAGAGACCGGTTCGGGGTGCTGTCGCGCCTTGAATACTATACGCAGGAAGAGCT
GACAGATATCGTGTCAAGAACGGCGGACGTTTTTGAAGTGGATATCGAAGAAGCTCCGGCTCTGGAGATCGCCAGACGGT
CAAGGGGAACGCCGCGGGTTGCAAACCGGCTTCTCAGAAGGGTGAGGGATTTTGCCCAGGTGCTCGGCGACAGCCGCATC
ACGGAAGAAATCGCTCACGACGCGCTCGAACGTCTTCAAGTCGATGCGCTCGGCCTTGACCATATCGACCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAGCGGCGGTCCCGTCGGCCTTGATACGATTTCCGCGACAATCGGCGAAGAACCGCATA
CAATAGAGGATGTGTACGAACCGTATCTGCTGCAGATCGGATTTCTCCAGAGGACGCCGAGGGGACGGGTCGTGACTCCG
GCTGTGTATGAACATTTTCGATTGGAGGCGCCCGCCCGTGACTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB AAA lid domain (AAA_lid_4)

(182-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

91.916

100

0.919
  ruvB Streptococcus pneumoniae TIGR4

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae R6

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae D39

60.736

97.605

0.593
  ruvB Synechocystis sp. PCC 6803

58.359

98.503

0.575
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518