Detailed information NTG0145718

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AAY24_RS13355
• Genome accession: NZ_CP011412
• Coordinates: 2908666..2909742 (+)
• Length: 358 a.a.
• NCBI ID: WP_046860111.1
• Uniprot ID: A0A0F7JZU9
• Organism: Sedimenticola thiotaurini strain SIP-G1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2903666..2914742

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAY24_RS13340 (AAY24_13340)	-	2904144..2905439 (+)	1296	WP_046860108.1	citrate synthase	-
AAY24_RS13345 (AAY24_13345)	-	2905436..2906041 (-)	606	WP_046860109.1	hypothetical protein	-
AAY24_RS13350 (AAY24_13350)	-	2906056..2908440 (-)	2385	WP_234422176.1	penicillin-binding protein 1A	-
AAY24_RS13355 (AAY24_13355)	pilM	2908666..2909742 (+)	1077	WP_046860111.1	pilus assembly protein PilM	Machinery gene
AAY24_RS13360 (AAY24_13360)	-	2909810..2910301 (+)	492	WP_234422177.1	PilN domain-containing protein	-
AAY24_RS13365 (AAY24_13365)	-	2910298..2910882 (+)	585	WP_046860113.1	type 4a pilus biogenesis protein PilO	-
AAY24_RS13370 (AAY24_13370)	-	2910882..2911415 (+)	534	WP_046860114.1	pilus assembly protein PilP	-
AAY24_RS13375 (AAY24_13375)	pilQ	2911480..2913612 (+)	2133	WP_046861309.1	type IV pilus secretin PilQ	-
AAY24_RS13380 (AAY24_13380)	aroK	2913789..2914307 (+)	519	WP_046860115.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 358 a.a.
• Molecular weight: 38321.19 Da
• Isoelectric Point: 4.3120

>NTDB_id=145718 AAY24_RS13355 WP_046860111.1 2908666..2909742(+) (pilM) [Sedimenticola thiotaurini strain SIP-G1]
MVPFFGSKKQAMLGLDISSTAVKLLELSKSSGRSGARYRVEAYAVEPLPANAVVEKNIADVDAVGQAIHNVVRRSGSKAR
QAAVAVSGSAVITKIITMPASLSDSEMESQIQLEADQYIPYPLEEVNLDFEILGSSEKNPEMMDVLLAASRSENVDDRVA
ALEIAGLTASVVDVEAYAMENAFTQIADQLPDGGDDQTVAIADIGATTTTLNVLRNGKIIYTREQNFGGRQLTEEIQRRY
GLSHEEAGMAKRQGGLPDNYVPEVLEPFKEAMGQQVSRSLQFFFSSSAYNSVDYIVLAGGSSSIPGVDELIEEKIGTPTI
VANPFANMSVSSNVKPQGLSNDAPSLMIACGLALRSFD

Nucleotide

• Length: 1077 bp

>NTDB_id=145718 AAY24_RS13355 WP_046860111.1 2908666..2909742(+) (pilM) [Sedimenticola thiotaurini strain SIP-G1]
ATGGTCCCTTTTTTCGGATCCAAGAAGCAGGCAATGCTTGGGCTGGATATCAGTTCGACGGCAGTAAAATTGCTTGAATT
GAGTAAGTCCTCAGGTAGATCGGGTGCACGGTACCGTGTGGAGGCCTACGCTGTAGAGCCCTTGCCGGCCAATGCGGTGG
TCGAGAAAAATATCGCCGATGTGGATGCTGTCGGGCAAGCGATCCACAATGTGGTTAGGCGCTCCGGCAGTAAGGCGCGC
CAGGCGGCTGTGGCGGTATCCGGTTCCGCAGTGATCACCAAGATCATCACTATGCCGGCCTCACTCTCCGATTCGGAGAT
GGAGAGCCAGATTCAACTTGAGGCCGATCAGTACATCCCCTATCCACTTGAGGAGGTGAATCTCGATTTTGAGATACTCG
GCTCATCGGAAAAGAATCCGGAAATGATGGATGTGTTGCTGGCGGCCTCCCGAAGTGAAAATGTTGATGATCGGGTGGCT
GCACTAGAGATTGCCGGGCTGACAGCAAGTGTAGTGGATGTGGAAGCCTACGCCATGGAGAACGCCTTTACCCAGATCGC
GGATCAACTGCCGGATGGCGGCGATGATCAGACAGTAGCAATTGCGGACATAGGTGCCACGACCACTACCCTGAATGTTC
TGCGCAATGGCAAGATTATCTATACAAGGGAACAGAATTTTGGCGGTCGTCAGTTAACCGAGGAGATTCAACGCCGGTAT
GGGCTTTCCCACGAAGAGGCTGGGATGGCCAAGCGGCAAGGCGGACTTCCGGATAATTATGTGCCCGAGGTGTTGGAGCC
TTTTAAAGAGGCTATGGGGCAACAGGTCAGCCGCTCATTGCAGTTCTTCTTCTCTTCAAGTGCCTACAACAGTGTTGACT
ATATCGTCCTCGCAGGCGGTAGTTCATCGATACCGGGAGTGGATGAATTGATAGAGGAGAAGATCGGTACTCCGACAATT
GTTGCCAATCCCTTTGCAAACATGTCTGTTTCATCCAACGTTAAGCCCCAGGGTTTAAGTAACGATGCGCCATCGCTGAT
GATTGCTTGCGGACTCGCATTAAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-357)

3D structure

• AlphaFold DB: A0A0F7JZU9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.352	100	0.534
comM	Acinetobacter nosocomialis M2	53.352	100	0.534
comM	Acinetobacter baylyi ADP1	52.514	100	0.525
pilM	Legionella pneumophila strain ERS1305867	47.207	100	0.472