Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AAV29_RS12565 Genome accession   NZ_CP011346
Coordinates   2607217..2608287 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain JJ-D34     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2602217..2613287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAV29_RS12515 (AAV29_12515) sipW 2602277..2602861 (-) 585 WP_046559873.1 signal peptidase I SipW -
  AAV29_RS12520 (AAV29_12520) tapA 2602833..2603504 (-) 672 WP_046559874.1 amyloid fiber anchoring/assembly protein TapA -
  AAV29_RS12525 (AAV29_12525) - 2603763..2604092 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  AAV29_RS12530 (AAV29_12530) - 2604132..2604311 (-) 180 WP_003153093.1 YqzE family protein -
  AAV29_RS12535 (AAV29_12535) comGG 2604368..2604745 (-) 378 WP_046559875.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAV29_RS12540 (AAV29_12540) comGF 2604746..2605141 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  AAV29_RS12545 (AAV29_12545) comGE 2605155..2605469 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAV29_RS12550 (AAV29_12550) comGD 2605453..2605890 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  AAV29_RS12555 (AAV29_12555) comGC 2605880..2606146 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  AAV29_RS12560 (AAV29_12560) comGB 2606193..2607230 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAV29_RS12565 (AAV29_12565) comGA 2607217..2608287 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  AAV29_RS12570 (AAV29_12570) - 2608479..2609429 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  AAV29_RS12575 (AAV29_12575) - 2609575..2610876 (+) 1302 WP_014305416.1 hemolysin family protein -
  AAV29_RS12580 (AAV29_12580) - 2610915..2611745 (-) 831 WP_003153080.1 STAS domain-containing protein -
  AAV29_RS12590 (AAV29_12590) - 2611951..2612331 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AAV29_RS12595 (AAV29_12595) - 2612567..2612812 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=144867 AAV29_RS12565 WP_003153083.1 2607217..2608287(-) (comGA) [Bacillus velezensis strain JJ-D34]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=144867 AAV29_RS12565 WP_003153083.1 2607217..2608287(-) (comGA) [Bacillus velezensis strain JJ-D34]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment