Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BIS30_RS16025 Genome accession   NZ_CP011051
Coordinates   3075979..3079683 (+) Length   1234 a.a.
NCBI ID   WP_003224421.1    Uniprot ID   -
Organism   Bacillus spizizenii strain T30     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3070979..3084683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIS30_RS16010 (BIS30_16010) - 3071425..3071754 (-) 330 WP_032727222.1 four-helix bundle copper-binding protein -
  BIS30_RS16015 (BIS30_16015) - 3071871..3072308 (-) 438 WP_003224414.1 ferritin family protein -
  BIS30_RS16020 (BIS30_16020) addB 3072492..3075992 (+) 3501 WP_003224417.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BIS30_RS16025 (BIS30_16025) addA 3075979..3079683 (+) 3705 WP_003224421.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BIS30_RS16030 (BIS30_16030) sbcD 3079755..3080930 (+) 1176 WP_003224424.1 exonuclease subunit SbcD -
  BIS30_RS16035 (BIS30_16035) sbcC 3080927..3084319 (+) 3393 WP_003224426.1 exonuclease subunit SbcC -
  BIS30_RS16040 (BIS30_16040) hlpB 3084333..3084635 (+) 303 WP_003224428.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141455.81 Da        Isoelectric Point: 5.0516

>NTDB_id=141806 BIS30_RS16025 WP_003224421.1 3075979..3079683(+) (addA) [Bacillus spizizenii strain T30]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDQLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIEMVLNGAKEKLQRA
LELTKVPGGPAPRADNFLDDLAQIDELIQHQDDFTELYKRVPAVSFKRVKAVKGDEFDPTLLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVRLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENETGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLETFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMEVLAENGAATHADISGHPARFAVRMIHSYD
LLDDDLEERTEEKSERLEAIRKGEPVPGTFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHIPTIEEAEQTVHKLYEKELLTEEQKTAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=141806 BIS30_RS16025 WP_003224421.1 3075979..3079683(+) (addA) [Bacillus spizizenii strain T30]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTCTCAACCGGCCAGGATATTCT
CGTAGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCAGCTTCTTGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAACGCCCCGGCTCGCTGCATATCAGGCGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATATGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGATCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGTGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGAGATGGTGCTTAACGGAGCGAAGGAAAAGCTTCAGCGCGCG
CTTGAACTGACGAAAGTGCCGGGCGGTCCCGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGATGATTTTACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGTGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTACACTCCTTGAGGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACGAGAAGTCCTGAACAGCATTTAAAAAGCCTAGCCGACATGAAGCCTGTTATTGAAACGCTCGTACG
GCTTGTCATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAATGAAACAGGTGAACGCGAGCCGAGCGAGGCAGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
TGAGGAAACAGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCAATTTACCGATTCAGGCTTGCGGAGCCGCTTCTAT
TCCTCTCCAAATACAAACGGTTTACAGAAAGTGGAGAAGGCACGGGACGGAAAATTGACTTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTCTATTTAAACAACTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATGAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGAGACGGATGCAGGCGAAGAAGCAGAAGAGCTGGAAACGGTGCAGTTTGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATTGTGAT
TTTGCTCCGCTCCATGCCGTGGGCACCGCAAATCATGGAGGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTTGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTACGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGGAAACGT
TCTACGGGCATCTTCAAAAATGGCGTGCATTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGAGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGATTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTCGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTTAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGGTTCGGCACAAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAAAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAGACTGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATTGGACCGGCTCTTGCGAGACACAGAGATATGGAGGTTTTGG
CTGAAAACGGTGCGGCAACGCATGCTGATATATCAGGACACCCGGCTCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTACTGGATGATGATCTGGAAGAAAGGACGGAAGAAAAAAGCGAGCGCTTAGAAGCGATCCGCAAAGGTGAACCAGTTCC
CGGCACGTTTGCATTTGATGAAAAAGCCCGCGAGCAGTTGAGCTGGACATACCCGCATCACGAGGTCACGCAAATTCGGA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCCGGGCCCCCGTTAAACCTGCT
GACGGAAGCATTCTGTACAGGCGCCCCGCTTTCATGATGAAAAAAGGCCTGACAGCGGCGGAGAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTTACACATATCCCAACGATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAACCGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCCAAGGAAATCTATCCTGATGC
ACAGGAGGCAGATGAACCGCTTTTAGTGCAGGGGATCATCGACTGTCTCTATGAAACTGAGGACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAGTTCCAACACGGCTTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTGTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.515

100

0.965

Multiple sequence alignment