Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PTRA_RS13620 Genome accession   NZ_CP011034
Coordinates   2935456..2936592 (-) Length   378 a.a.
NCBI ID   WP_058374184.1    Uniprot ID   A0A0U2V8U7
Organism   Pseudoalteromonas translucida KMM 520     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2930456..2941592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTRA_RS13590 (PTRA_a3125) - 2930969..2931862 (+) 894 WP_058374180.1 hypothetical protein -
  PTRA_RS13595 (PTRA_a3126) - 2931938..2932417 (+) 480 WP_058374181.1 hypothetical protein -
  PTRA_RS13600 (PTRA_a3127) rsmE 2932490..2933224 (+) 735 WP_011329246.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PTRA_RS13605 (PTRA_a3128) gshB 2933279..2934229 (+) 951 WP_058374182.1 glutathione synthase -
  PTRA_RS13610 (PTRA_a3130) - 2934370..2934927 (+) 558 WP_058374183.1 YqgE/AlgH family protein -
  PTRA_RS13615 (PTRA_a3131) ruvX 2934940..2935389 (+) 450 WP_011329249.1 Holliday junction resolvase RuvX -
  PTRA_RS13620 (PTRA_a3132) pilU 2935456..2936592 (-) 1137 WP_058374184.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PTRA_RS13625 (PTRA_a3133) pilT 2936602..2937642 (-) 1041 WP_041454575.1 type IV pilus twitching motility protein PilT Machinery gene
  PTRA_RS13630 (PTRA_a3134) - 2937645..2938358 (+) 714 WP_058374185.1 YggS family pyridoxal phosphate-dependent enzyme -
  PTRA_RS13635 (PTRA_a3135) proC 2938376..2939197 (+) 822 WP_011329253.1 pyrroline-5-carboxylate reductase -
  PTRA_RS13640 (PTRA_a3136) - 2939210..2939743 (+) 534 WP_058374186.1 YggT family protein -
  PTRA_RS13645 (PTRA_a3137) - 2939801..2940244 (+) 444 WP_058374187.1 DUF4426 domain-containing protein -
  PTRA_RS13650 (PTRA_a3138) - 2940252..2940851 (+) 600 WP_058374188.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41823.84 Da        Isoelectric Point: 5.8295

>NTDB_id=141645 PTRA_RS13620 WP_058374184.1 2935456..2936592(-) (pilU) [Pseudoalteromonas translucida KMM 520]
MALSLNNFLQIMIDKKGSDLFVSSQLPISAKINGELTPLDDEPLSDEQSLELVESAMSEKQKAEFHATKECNFAIATDEG
RFRISAFWQRDKAGMVIRRIVTKIPEIGELGLPSTLTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGQGRVAAIEILLNSPMVAELIKNGDIGGIKETMAKSKEMGMQTFDQ
ALFELYKQQRINYADALHHADSANDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNIS

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=141645 PTRA_RS13620 WP_058374184.1 2935456..2936592(-) (pilU) [Pseudoalteromonas translucida KMM 520]
ATGGCTTTATCTTTAAATAACTTTTTACAAATAATGATAGATAAAAAAGGCTCTGATTTATTTGTTTCAAGCCAACTGCC
TATCAGCGCTAAAATTAATGGTGAATTAACCCCACTTGATGATGAGCCGCTCAGCGATGAGCAATCACTTGAGTTGGTTG
AATCGGCAATGAGTGAAAAACAAAAAGCCGAGTTTCATGCAACCAAAGAATGCAACTTTGCGATTGCCACTGACGAAGGG
CGTTTTCGTATTTCGGCATTTTGGCAGCGCGATAAAGCGGGCATGGTTATTCGTCGTATTGTTACTAAAATACCTGAAAT
TGGCGAACTTGGGCTGCCGTCTACACTTACCGATGTGATTATGTCGAAACGTGGTTTAGTGCTATTTGTGGGGGGAACAG
GTACGGGTAAGTCAACCTCACTTGCTGCACTTATTGGCTATAGAAACCGTAATCAGCGCGGTCATATATTAACCATTGAA
GACCCGATTGAGTTTGTACACGAACATCGTAAAAGTATTATTACTCAACGCGAAGTAGGGCTAGATACCGAAAGCTTTGA
ATCAGCACTTAAAAGCTCGTTACGCCAAGCACCCGACGTAATATTAATAGGCGAAATACGCTCACAAGAAACCATGGAGT
ATGCATTAAGCTTTGCAGAAACAGGGCATTTATGTGTGGCTACCTTGCATGCAAACAACGCTAACCAAGCGATAGATCGG
ATCATGCATTTAGTGCCAAAAGAAAAACACGATAAGCTTAAATACGATCTAGCATTAAATTTACGCGCTATTATTGCCCA
GCAGCTGATCCCCACATCTGACGGGCAAGGCCGAGTTGCTGCTATTGAAATATTACTTAATTCACCTATGGTTGCAGAGC
TAATTAAAAATGGTGATATAGGCGGTATTAAAGAAACCATGGCTAAATCGAAAGAAATGGGCATGCAGACCTTTGATCAG
GCCTTATTTGAATTGTATAAACAGCAACGAATCAATTATGCAGATGCGTTGCACCATGCCGATTCGGCGAACGATTTACG
CTTAATGATAAAGCTGCGCAACAATGAGCAGCAAGGGGCGGGCTTTTTACAAGGGGTCACTATTGATGGCCTTGATGATA
AAGGCAATATTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U2V8U7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.333

99.206

0.569
  pilU Acinetobacter baylyi ADP1

56.499

99.735

0.563
  pilU Vibrio cholerae strain A1552

57.493

97.09

0.558
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.767

95.503

0.418

Multiple sequence alignment