Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PISS_RS13085 Genome accession   NZ_CP011030
Coordinates   2819194..2820330 (-) Length   378 a.a.
NCBI ID   WP_013465850.1    Uniprot ID   A0AA37S1K5
Organism   Pseudoalteromonas issachenkonii strain KMM 3549     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2814194..2825330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PISS_RS13055 (PISS_a2912) - 2814709..2815602 (+) 894 WP_058155236.1 hypothetical protein -
  PISS_RS13060 (PISS_a2913) - 2815680..2816153 (+) 474 WP_028835646.1 hypothetical protein -
  PISS_RS13065 (PISS_a2914) rsmE 2816224..2816958 (+) 735 WP_028835645.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PISS_RS13070 (PISS_a2915) gshB 2817025..2817975 (+) 951 WP_013465847.1 glutathione synthase -
  PISS_RS13075 (PISS_a2916) - 2818111..2818668 (+) 558 WP_024600819.1 YqgE/AlgH family protein -
  PISS_RS13080 (PISS_a2917) ruvX 2818686..2819135 (+) 450 WP_013465849.1 Holliday junction resolvase RuvX -
  PISS_RS13085 (PISS_a2919) pilU 2819194..2820330 (-) 1137 WP_013465850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PISS_RS13090 (PISS_a2920) pilT 2820340..2821380 (-) 1041 WP_013465851.1 type IV pilus twitching motility protein PilT Machinery gene
  PISS_RS13095 (PISS_a2921) - 2821383..2822096 (+) 714 WP_024600821.1 YggS family pyridoxal phosphate-dependent enzyme -
  PISS_RS13100 (PISS_a2922) proC 2822114..2822935 (+) 822 WP_024600822.1 pyrroline-5-carboxylate reductase -
  PISS_RS13105 (PISS_a2923) - 2822962..2823495 (+) 534 WP_058155237.1 YggT family protein -
  PISS_RS13110 (PISS_a2924) - 2823553..2823996 (+) 444 WP_058155238.1 DUF4426 domain-containing protein -
  PISS_RS13115 (PISS_a2926) - 2823999..2824598 (+) 600 WP_016900586.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41994.04 Da        Isoelectric Point: 6.2895

>NTDB_id=141595 PISS_RS13085 WP_013465850.1 2819194..2820330(-) (pilU) [Pseudoalteromonas issachenkonii strain KMM 3549]
MTLSLNHFLQIMIDKKGSDLFVSSQLPISAKINGELTPLNDDKLTDEQALELVESAMSEKQKAEFHNTKECNFAIATSEG
RFRISAFWQRDRAGMVIRRIVTKIPDVKELGLPSALTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRVAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=141595 PISS_RS13085 WP_013465850.1 2819194..2820330(-) (pilU) [Pseudoalteromonas issachenkonii strain KMM 3549]
ATGACTTTATCTTTAAATCACTTTTTACAAATAATGATCGACAAAAAAGGCTCTGATTTATTTGTATCGAGCCAACTTCC
TATTAGCGCTAAAATTAACGGCGAGCTAACTCCTCTTAATGACGATAAATTAACAGATGAGCAAGCGCTAGAGTTAGTTG
AATCAGCGATGAGTGAAAAACAAAAAGCAGAGTTTCATAACACTAAAGAATGTAACTTTGCGATTGCAACTAGCGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGCGACAGAGCGGGCATGGTTATTCGCCGAATTGTCACTAAGATTCCCGACGT
TAAAGAGCTTGGATTACCTTCTGCATTGACCGATGTGATTATGTCAAAGCGCGGACTCGTGTTATTTGTAGGTGGTACGG
GAACCGGTAAATCAACGTCTTTGGCGGCACTTATTGGTTATAGAAATCGCAATCAACGTGGGCATATTTTAACCATAGAA
GATCCGATTGAATTTGTGCATGAACATCAAAAAAGTATTATTACCCAACGTGAAGTGGGTTTAGATACTGAAAGCTTTGA
ATCGGCACTTAAAAGCTCTTTGCGCCAAGCGCCTGATGTTATTTTAATTGGTGAAATTCGTTCGCAAGAAACCATGGAAT
ATGCGTTGAGCTTTGCAGAAACGGGTCATTTATGTGTTGCCACCCTGCATGCCAACAATGCTAACCAAGCTATTGACCGC
ATTATGCACTTAGTACCAAAAGAAAAGCACGATAAGCTTAAATATGACTTAGCATTAAATTTACGCGCTATCATTGCTCA
GCAACTTATTCCTACCTCTGATGGGGAAGGGCGTGTTGCTGCCATTGAGGTATTACTTAATACGCCAATGGTCAGTGAGC
TAATTAAAAACGGTAATATTGGTGGCATTAAAGAAACCATGGCTAAATCAACAGAGATGGGCATGCAAACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGACAGCAACGTATTAATTATGCCGATGCATTGCATCATGCGGATTCGCCAAATGACTTACG
CTTGATGATTAAACTACGTAATAACGAGCAGCAAGGCGCAGGCTTCTTACAAGGTGTGACCATTGATGGCCTTGATGACA
AAGGCAATATGACTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

57.56

99.735

0.574
  pilU Pseudomonas stutzeri DSM 10701

57.6

99.206

0.571
  pilU Vibrio cholerae strain A1552

56.131

97.09

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

95.238

0.399

Multiple sequence alignment