Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VN11_RS05230 Genome accession   NZ_CP011010
Coordinates   1130180..1131343 (+) Length   387 a.a.
NCBI ID   WP_049434422.1    Uniprot ID   A0AA43BPT5
Organism   Stenotrophomonas maltophilia strain ISMMS3 isolate Patient     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1125180..1136343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN11_RS05200 (VN11_05215) soxR 1125324..1125758 (+) 435 WP_008267329.1 redox-sensitive transcriptional activator SoxR -
  VN11_RS05205 (VN11_05220) - 1125841..1126443 (-) 603 WP_053448993.1 short chain dehydrogenase -
  VN11_RS05210 (VN11_05225) - 1126544..1127422 (+) 879 WP_053448994.1 LysR family transcriptional regulator -
  VN11_RS05215 (VN11_05230) proC 1127459..1128280 (-) 822 WP_053448995.1 pyrroline-5-carboxylate reductase -
  VN11_RS05220 (VN11_05235) - 1128299..1128976 (-) 678 WP_053448996.1 YggS family pyridoxal phosphate-dependent enzyme -
  VN11_RS05225 (VN11_05240) pilT 1129064..1130101 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  VN11_RS05230 (VN11_05245) pilU 1130180..1131343 (+) 1164 WP_049434422.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VN11_RS05235 (VN11_05250) - 1131630..1132613 (+) 984 WP_053448997.1 aldo/keto reductase -
  VN11_RS05240 (VN11_05255) - 1132689..1133588 (-) 900 WP_053448998.1 LysR family transcriptional regulator -
  VN11_RS05245 (VN11_05260) - 1133914..1134126 (+) 213 WP_053448999.1 DUF4287 domain-containing protein -
  VN11_RS05250 (VN11_05265) - 1134238..1134945 (+) 708 WP_049456208.1 YitT family protein -
  VN11_RS05255 (VN11_05270) - 1135019..1135561 (-) 543 WP_053449000.1 DNA-3-methyladenine glycosylase I -
  VN11_RS05260 (VN11_05275) - 1135651..1136217 (+) 567 WP_006422622.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43186.63 Da        Isoelectric Point: 6.6615

>NTDB_id=141416 VN11_RS05230 WP_049434422.1 1130180..1131343(+) (pilU) [Stenotrophomonas maltophilia strain ISMMS3 isolate Patient]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=141416 VN11_RS05230 WP_049434422.1 1130180..1131343(+) (pilU) [Stenotrophomonas maltophilia strain ISMMS3 isolate Patient]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACCGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACCCAGA
CCCCGCTCACGCCGCAGCAGAGTCGCGACCTGGTGCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAGTGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGTGTCAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGCCGCATCGAGACGCGCATTCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCTACCGGTACCGGTAAATCGACCTCGCTGGCAGCGATGATCGGTTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCAGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGACACCTGGTGCTGTGC
ACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGTTGCT
GATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGATTCCTTCGCCCGATGGCCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGATTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGA
GGACGCACTGCGCTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCC
TGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

94.832

0.641
  pilU Acinetobacter baylyi ADP1

63.866

92.248

0.589
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395

Multiple sequence alignment