Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   B723_RS00360 Genome accession   NZ_CP010945
Coordinates   72420..73757 (+) Length   445 a.a.
NCBI ID   WP_017340841.1    Uniprot ID   A0A0K1QGM4
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 67420..78757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS00335 (B723_00335) pgeF 67628..68353 (-) 726 WP_017340836.1 peptidoglycan editing factor PgeF -
  B723_RS00340 (B723_00340) rluD 68350..69312 (-) 963 WP_017340837.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  B723_RS00345 (B723_00345) - 69464..70486 (+) 1023 WP_017340838.1 outer membrane protein assembly factor BamD -
  B723_RS00350 (B723_00350) - 70611..70844 (+) 234 WP_017340839.1 PP0621 family protein -
  B723_RS00355 (B723_00355) pilS 70834..72423 (+) 1590 WP_017340840.1 ATP-binding protein Regulator
  B723_RS00360 (B723_00360) pilR 72420..73757 (+) 1338 WP_017340841.1 sigma-54 dependent transcriptional regulator Regulator
  B723_RS00365 (B723_00365) thiO 73777..74877 (-) 1101 WP_017340842.1 glycine oxidase ThiO -
  B723_RS00370 (B723_00370) - 74934..75332 (-) 399 WP_017340843.1 type IV pilin protein -
  B723_RS00375 (B723_00375) - 75347..78451 (-) 3105 WP_031319127.1 PilC/PilY family type IV pilus protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49820.22 Da        Isoelectric Point: 6.6151

>NTDB_id=140790 B723_RS00360 WP_017340841.1 72420..73757(+) (pilR) [Pseudomonas fluorescens NCIMB 11764]
MNTSPQQKVLIVDDEPDIRELLEITLGRMKLDTFSARNLGEAQTLLKRETFDLCLTDMRLPDGTGLELVQHIQQRYPQLP
VAMITAYGNLETAINALKAGAFDFLTKPVDLACLRELVTSALRMPAPGATVDRRLLGDSLPMRNLRKQIDKLARSQAPVY
ISGESGSGKELVARLIHEQGSRANQPFIPVNCGAIPSELMESEFFGHRKGSFSGAIEDKPGLFQAAHGGTLFLDEVADLP
LAMQVKLLRAIQEKAVRSVGGQQETVVDVRILCATHKDLAAEVAAERFRQDLYYRLNVIELRVPPLRERRDDIESLASHV
LMRLAARAGEPATKLQPQALEALKNYRFPGNVRELENMLERAHTLCENKQIEASDLRLAEGNCTADSGVQDLTQIDNLEV
YLDNVERKLILQALEETRWNRTAAAQRLNLSFRSMRYRLKKFGLD

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=140790 B723_RS00360 WP_017340841.1 72420..73757(+) (pilR) [Pseudomonas fluorescens NCIMB 11764]
TTGAACACCAGCCCCCAGCAAAAAGTCCTGATCGTCGACGACGAACCGGATATCCGCGAACTCCTGGAAATCACCCTGGG
ACGAATGAAACTCGACACGTTCAGCGCCCGCAACCTTGGCGAAGCCCAGACCTTGTTGAAGCGTGAAACATTCGATCTGT
GCCTGACCGACATGCGTCTTCCGGACGGCACCGGCCTTGAGCTGGTGCAACACATTCAGCAACGCTATCCCCAACTGCCG
GTTGCCATGATCACCGCTTATGGCAACCTGGAAACGGCGATCAACGCGCTCAAGGCTGGTGCTTTCGACTTCCTGACCAA
ACCGGTGGACCTCGCCTGCCTGCGCGAACTGGTCACCAGTGCACTGCGTATGCCCGCGCCAGGCGCCACCGTCGATCGTC
GTTTGCTGGGCGACTCGTTGCCCATGCGCAATTTGCGTAAACAGATCGACAAGCTGGCCCGCAGTCAGGCGCCGGTGTAC
ATCAGCGGCGAGTCCGGCAGCGGCAAGGAGTTGGTCGCCCGGCTGATCCATGAACAAGGGTCACGCGCCAACCAGCCGTT
TATCCCGGTGAACTGCGGGGCAATCCCGTCGGAGCTGATGGAGAGCGAATTTTTCGGGCATCGCAAAGGCAGCTTCAGCG
GCGCCATCGAAGACAAACCCGGACTGTTCCAGGCAGCCCATGGCGGCACGCTGTTCCTCGACGAAGTGGCTGATTTGCCG
CTGGCGATGCAAGTCAAACTGTTGCGGGCGATTCAGGAAAAAGCCGTTCGCAGCGTGGGCGGGCAACAGGAAACCGTGGT
CGATGTGCGCATCCTCTGCGCCACTCACAAAGACCTCGCCGCCGAAGTCGCCGCTGAACGCTTTCGCCAGGATTTGTACT
ACCGGCTCAACGTCATCGAACTACGTGTTCCGCCCTTGCGCGAACGCCGCGACGACATTGAATCGTTGGCCAGCCATGTT
CTCATGCGTCTGGCAGCCCGCGCCGGCGAGCCCGCCACGAAACTCCAACCGCAAGCCCTTGAAGCGCTGAAAAACTATCG
TTTTCCGGGCAACGTGCGCGAGCTGGAGAACATGCTCGAACGGGCTCACACTTTGTGCGAAAACAAGCAGATCGAAGCCA
GCGACCTGCGACTGGCCGAAGGCAACTGCACCGCAGACAGTGGGGTGCAGGACCTGACGCAGATCGACAATCTGGAGGTC
TATCTGGATAACGTCGAACGCAAGCTCATCCTCCAGGCCCTGGAGGAAACCCGCTGGAACCGCACGGCGGCGGCTCAGCG
GTTGAATTTGTCGTTTCGGTCGATGCGTTACAGGCTGAAGAAATTCGGGTTGGATTGA

Domains

Predicted by InterproScan.

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)

Response regulator receiver domain (Response_reg)

(9-118)

Sigma-54 interaction domain (Sigma54_activat)

(136-301)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1QGM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

78.33

99.551

0.78
  pilR Acinetobacter baumannii strain A118

49.244

100

0.512
  luxO Vibrio cholerae strain A1552

36.261

99.775

0.362

Multiple sequence alignment