Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   B723_RS00355 Genome accession   NZ_CP010945
Coordinates   70834..72423 (+) Length   529 a.a.
NCBI ID   WP_017340840.1    Uniprot ID   -
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 65834..77423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS00335 (B723_00335) pgeF 67628..68353 (-) 726 WP_017340836.1 peptidoglycan editing factor PgeF -
  B723_RS00340 (B723_00340) rluD 68350..69312 (-) 963 WP_017340837.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  B723_RS00345 (B723_00345) - 69464..70486 (+) 1023 WP_017340838.1 outer membrane protein assembly factor BamD -
  B723_RS00350 (B723_00350) - 70611..70844 (+) 234 WP_017340839.1 PP0621 family protein -
  B723_RS00355 (B723_00355) pilS 70834..72423 (+) 1590 WP_017340840.1 ATP-binding protein Regulator
  B723_RS00360 (B723_00360) pilR 72420..73757 (+) 1338 WP_017340841.1 sigma-54 dependent transcriptional regulator Regulator
  B723_RS00365 (B723_00365) thiO 73777..74877 (-) 1101 WP_017340842.1 glycine oxidase ThiO -
  B723_RS00370 (B723_00370) - 74934..75332 (-) 399 WP_017340843.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58473.22 Da        Isoelectric Point: 7.0021

>NTDB_id=140789 B723_RS00355 WP_017340840.1 70834..72423(+) (pilS) [Pseudomonas fluorescens NCIMB 11764]
MIAETSSPGSKQAQRLLRLYHLYRLSVGITLVLLISSNLDNQLLTYSNDDLLRSGSWLYLVLNILLVVFLENTRRPAQLF
SLALIDVLLLCGLFYAAGGVASAIGNLLIVSVAISNALLRSRIGLLIAAVGALGIVGLSFLLSFSHPATPNDYLQVGTLG
ALCFAAALLVQGLIQRVEVSETLAEQRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSAQALLGRDNLEGRRID
DCSPALVERLQLWRNNPTLRPQSLKITSSGLELQPSFIALDQSPHHQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNGADRRLTQIIQDHSQRMNRVIENVLQLSRRQQAAPQRLDLKPWLEQFVAESREGALDR
QKIHLHIGSGDFKTLMDPNQLTQILDNLLRNAWRHSAQTHDQAQVWLDLFIDPDTQLPIIEVLDDGAGVAPDQQVHLFEP
FFTTSAQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=140789 B723_RS00355 WP_017340840.1 70834..72423(+) (pilS) [Pseudomonas fluorescens NCIMB 11764]
GTGATCGCTGAGACGTCCAGTCCCGGCAGCAAACAGGCCCAGCGACTGCTGCGCCTTTATCATCTCTACCGTTTAAGTGT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACCTCGACAACCAGTTGCTGACCTATTCCAATGACGACCTGTTGCGCA
GTGGCAGCTGGTTGTATCTGGTCCTGAACATTCTGCTGGTGGTCTTCCTTGAGAACACCCGCCGTCCGGCACAACTGTTC
AGCCTGGCGCTGATCGATGTCCTGCTGCTGTGCGGCCTGTTCTATGCCGCCGGTGGTGTCGCCAGCGCCATTGGCAACCT
GCTGATCGTCTCGGTGGCCATCAGCAATGCCTTGTTGCGAAGTCGTATCGGCCTGCTGATTGCCGCCGTCGGCGCACTCG
GCATCGTCGGGCTGAGTTTTCTGCTGAGCTTCAGCCATCCCGCCACCCCTAACGATTACCTGCAAGTCGGTACGCTCGGT
GCGCTGTGTTTTGCCGCGGCATTGCTGGTGCAAGGGCTGATCCAGCGGGTGGAAGTCAGCGAAACCCTCGCCGAGCAACG
GGCCAGTGAAGTCGTGGGCCTGGAAGCCCTCAATGCGTTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTGCTCGACG
AACAGCGGCGAGTGCAACTGGCCAATCACAGCGCCCAGGCCCTGCTGGGCCGGGACAACCTCGAAGGCCGAAGGATCGAT
GACTGCTCACCCGCTCTCGTCGAGCGCCTGCAACTGTGGCGGAATAACCCGACGCTGCGCCCCCAAAGCCTGAAAATCAC
CAGCAGCGGCCTGGAACTGCAACCGAGCTTCATTGCGCTGGACCAAAGCCCGCACCACCAGACCCTGGTGTTTCTCGAAG
ACCTCGCGCAAATCGCCCAACAGGCTCAACAACTGAAACTCGCCGCCCTTGGCCGCCTGACTGCCGGTATCGCCCACGAA
ATACGCAATCCGCTGGGCGCCATTAGTCATGCCGCCCAGCTATTGCGGGAATCCGAGGAACTGAACGGCGCGGACCGACG
TCTGACCCAGATTATTCAAGATCACTCTCAGCGTATGAATCGGGTTATCGAAAACGTCCTGCAACTGTCCCGCCGCCAAC
AAGCCGCGCCGCAACGGCTCGATCTGAAGCCGTGGCTGGAACAATTCGTTGCAGAAAGTCGCGAAGGCGCGCTCGACCGC
CAAAAAATTCATCTGCATATCGGCTCGGGCGACTTCAAAACACTGATGGACCCGAACCAGTTGACCCAGATTCTCGACAA
CCTGCTGCGCAATGCCTGGCGCCACAGCGCTCAAACCCATGATCAGGCGCAGGTCTGGCTTGATCTGTTCATCGACCCCG
ACACCCAGTTGCCCATCATCGAAGTGTTGGACGATGGCGCCGGTGTGGCGCCGGATCAGCAAGTGCATCTGTTCGAACCG
TTTTTCACCACCAGCGCCCAAGGCACAGGTCTGGGGCTTTATCTGTCCCGTGAACTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAGCCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAGAGTTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.189

100

0.603

Multiple sequence alignment