Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   TO73_RS02695 Genome accession   NZ_CP010822
Coordinates   464431..465030 (-) Length   199 a.a.
NCBI ID   WP_003044369.1    Uniprot ID   -
Organism   Thermus aquaticus Y51MC23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459431..470030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO73_RS02675 (TO73_0552) - 459461..460015 (-) 555 WP_003044357.1 shikimate kinase -
  TO73_RS02680 (TO73_0553) aroC 460028..461179 (-) 1152 WP_003044360.1 chorismate synthase -
  TO73_RS02685 (TO73_0554) pilQ 461238..463493 (-) 2256 WP_003044363.1 secretin N-terminal domain-containing protein Machinery gene
  TO73_RS02690 (TO73_0555) pilW 463505..464434 (-) 930 WP_003044366.1 hypothetical protein Machinery gene
  TO73_RS02695 (TO73_0556) pilO 464431..465030 (-) 600 WP_003044369.1 type 4a pilus biogenesis protein PilO Machinery gene
  TO73_RS02700 (TO73_0557) pilN 465020..465643 (-) 624 WP_003044372.1 competence protein Machinery gene
  TO73_RS02705 (TO73_0558) pilM 465636..466769 (-) 1134 WP_003044375.1 type IV pilus assembly protein PilM Machinery gene
  TO73_RS02710 (TO73_0559) - 467048..468052 (-) 1005 WP_003044377.1 homoisocitrate dehydrogenase -
  TO73_RS02715 (TO73_0560) - 468110..468604 (-) 495 WP_040684158.1 DUF5317 domain-containing protein -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 21950.30 Da        Isoelectric Point: 10.2195

>NTDB_id=140099 TO73_RS02695 WP_003044369.1 464431..465030(-) (pilO) [Thermus aquaticus Y51MC23]
MLARLGQREWALIAMALVAVAALLWYFYGIVPLRQETEGVRQEINALIPEREKGRRAQRALPELRATIATLQAERQAFLR
ALPKEERLAQVLSEILGEATRSGVTVRSLNRSPTAAPVPEVRAVNLALALEAPFPETFTYLRRLEALSRFSTISGLNLSV
QGQEQNPTLSTSLTLTLYMLAKGLEAPSQGQTQSQGGER

Nucleotide


Download         Length: 600 bp        

>NTDB_id=140099 TO73_RS02695 WP_003044369.1 464431..465030(-) (pilO) [Thermus aquaticus Y51MC23]
GTGCTCGCTAGGCTGGGACAGCGGGAGTGGGCCCTCATCGCCATGGCCCTGGTGGCGGTGGCGGCCCTCCTCTGGTACTT
CTACGGCATCGTCCCCCTCCGCCAGGAAACGGAAGGGGTGCGCCAGGAGATCAACGCCCTCATCCCCGAGCGGGAGAAGG
GGCGCCGGGCCCAGCGGGCCCTTCCCGAGCTCAGGGCCACCATCGCCACCCTCCAAGCGGAAAGGCAGGCCTTCCTCCGG
GCCCTGCCCAAAGAGGAACGCCTAGCCCAGGTCCTCTCGGAGATCCTGGGGGAGGCCACCCGTAGCGGGGTCACGGTGCG
CTCCCTGAACCGCTCCCCCACCGCGGCTCCGGTGCCGGAGGTGCGGGCGGTGAACCTGGCCCTGGCCCTCGAGGCCCCCT
TCCCGGAGACCTTCACCTACCTGAGGCGCCTGGAAGCCCTCTCCCGCTTCAGCACCATCTCCGGGCTGAACCTGAGCGTG
CAGGGCCAGGAGCAAAACCCCACGCTCTCCACCAGCCTGACCCTCACCCTCTACATGCTGGCCAAGGGCCTGGAGGCCCC
AAGCCAGGGACAGACCCAGTCCCAGGGAGGTGAGCGGTGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(46-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Thermus thermophilus HB27

72.917

96.482

0.704

Multiple sequence alignment