Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   TO73_RS02705 Genome accession   NZ_CP010822
Coordinates   465636..466769 (-) Length   377 a.a.
NCBI ID   WP_003044375.1    Uniprot ID   -
Organism   Thermus aquaticus Y51MC23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 460636..471769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO73_RS02685 (TO73_0554) pilQ 461238..463493 (-) 2256 WP_003044363.1 secretin N-terminal domain-containing protein Machinery gene
  TO73_RS02690 (TO73_0555) pilW 463505..464434 (-) 930 WP_003044366.1 hypothetical protein Machinery gene
  TO73_RS02695 (TO73_0556) pilO 464431..465030 (-) 600 WP_003044369.1 type 4a pilus biogenesis protein PilO Machinery gene
  TO73_RS02700 (TO73_0557) pilN 465020..465643 (-) 624 WP_003044372.1 competence protein Machinery gene
  TO73_RS02705 (TO73_0558) pilM 465636..466769 (-) 1134 WP_003044375.1 type IV pilus assembly protein PilM Machinery gene
  TO73_RS02710 (TO73_0559) - 467048..468052 (-) 1005 WP_003044377.1 homoisocitrate dehydrogenase -
  TO73_RS02715 (TO73_0560) - 468110..468604 (-) 495 WP_040684158.1 DUF5317 domain-containing protein -
  TO73_RS02720 (TO73_0561) - 468655..470076 (-) 1422 WP_040684159.1 HD-GYP domain-containing protein -
  TO73_RS02735 (TO73_0566) - 470932..471297 (+) 366 WP_003044388.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41227.71 Da        Isoelectric Point: 4.4258

>NTDB_id=140101 TO73_RS02705 WP_003044375.1 465636..466769(-) (pilM) [Thermus aquaticus Y51MC23]
MFSSFSRLFKPRVEALGLEIGAGSLKLVELSGYPPALKALAARPLPPGTLVEGVVAEPNALSQEIRELLAEARTRKRYVV
SAVPNPSVILRTLQVPKMPPKEMEEAVRWEAERYIPFPIDEVVLDFAPLDPLAEVPEGEQVEVLVAAARQEAVASLIEAL
RGAGLIPVVLDVKPFAGLYPLEAELTRDPEQVTVAVEIGAESTSLVLTKGDRPLAVRILTLSGKDFTEAIAKGFGLDLLT
AEEVKRTYGLATIPTEDEELLLDFDVERERYSPARIYDAIRPVLVDLTQEIRRSLEFFRVQLGDVQPEVGYLYGGGSRLR
GLAALLTDTLGVSFVTLDPWQGIQVDPKRFDLEKLKELGAEFMVPVGLALRGVGPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=140101 TO73_RS02705 WP_003044375.1 465636..466769(-) (pilM) [Thermus aquaticus Y51MC23]
GTGTTTTCAAGTTTTAGCAGGCTATTCAAACCTCGGGTGGAGGCCCTGGGGCTGGAGATCGGGGCGGGGAGCCTCAAGCT
GGTGGAGCTTTCCGGCTACCCCCCGGCCCTCAAGGCCCTAGCCGCCCGGCCCCTGCCCCCAGGAACCCTGGTGGAAGGGG
TGGTGGCGGAGCCTAACGCGCTCAGCCAGGAGATCCGGGAACTCCTGGCCGAGGCCCGCACGAGGAAGCGCTATGTGGTC
TCAGCCGTCCCCAACCCCAGCGTGATCCTGCGCACCCTGCAGGTGCCCAAGATGCCCCCCAAGGAGATGGAGGAGGCCGT
GCGCTGGGAGGCGGAGCGCTACATCCCCTTCCCCATAGACGAGGTGGTCCTGGACTTCGCCCCCCTGGACCCCCTGGCCG
AGGTGCCAGAAGGGGAGCAGGTGGAGGTCTTGGTGGCGGCCGCCCGCCAGGAGGCGGTGGCCTCCCTTATAGAGGCCCTG
AGGGGGGCGGGCCTCATTCCCGTTGTCCTGGACGTAAAACCCTTCGCCGGCCTCTACCCCCTGGAAGCCGAGCTCACCAG
GGACCCCGAGCAGGTCACGGTCGCCGTGGAGATCGGCGCCGAGAGCACCAGCCTGGTCCTCACCAAGGGGGACCGCCCCC
TGGCGGTGCGGATCCTGACCCTCTCCGGCAAGGACTTCACCGAGGCCATCGCTAAGGGCTTTGGCCTGGACCTCCTCACC
GCCGAGGAGGTGAAGCGCACCTACGGCCTCGCCACCATCCCCACCGAGGACGAGGAGCTCCTCCTGGACTTTGACGTGGA
GCGGGAGCGGTACAGCCCGGCCCGCATCTACGACGCCATCCGCCCAGTGCTGGTGGACCTCACCCAGGAGATCCGCCGGA
GCCTGGAGTTCTTCCGGGTGCAGCTGGGGGACGTGCAGCCGGAGGTGGGCTACCTTTACGGCGGGGGTAGCCGCCTCCGG
GGCCTCGCCGCCCTCCTCACCGACACCCTGGGGGTGAGCTTCGTCACCCTGGACCCCTGGCAGGGCATCCAGGTGGACCC
CAAGCGCTTTGACCTGGAGAAGCTAAAGGAGCTGGGCGCCGAGTTCATGGTGCCCGTGGGCCTGGCCTTGAGGGGGGTGG
GTCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

76.923

100

0.769

Multiple sequence alignment