Detailed information    

experimental Experimentally validated

Overview

Name   recN   Type   Machinery gene
Locus tag   OK783_RS01640 Genome accession   NZ_CP115654
Coordinates   315526..317199 (+) Length   557 a.a.
NCBI ID   WP_003687706.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain FA1090     
Function   homologous recombination   
Homologous recombination

Function

The recN mutant was decreased in DNA transformation efficiency but not pilus-dependent variation frequency,


Genomic Context

Location: 310526..322199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OK783_RS01615 (OK783_01615) ptsN 311367..311816 (+) 450 WP_003687701.1 PTS IIA-like nitrogen regulatory protein PtsN -
  OK783_RS01620 (OK783_01620) hprK 311820..312782 (+) 963 WP_003687702.1 HPr(Ser) kinase/phosphatase -
  OK783_RS01625 (OK783_01625) rapZ 312763..313617 (+) 855 WP_003687703.1 RNase adapter RapZ -
  OK783_RS01630 (OK783_01630) ylqF 313698..314639 (+) 942 WP_003687704.1 ribosome biogenesis GTPase YlqF -
  OK783_RS01635 (OK783_01635) scpB 314740..315342 (+) 603 WP_002235622.1 SMC-Scp complex subunit ScpB -
  OK783_RS01640 (OK783_01640) recN 315526..317199 (+) 1674 WP_003687706.1 DNA repair protein RecN Machinery gene
  OK783_RS01655 (OK783_01655) mnmC 317944..319563 (-) 1620 WP_003687708.1 FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC -
  OK783_RS01660 (OK783_01660) - 319683..320051 (+) 369 WP_003687709.1 gamma-butyrobetaine hydroxylase-like domain-containing protein -
  OK783_RS01665 (OK783_01665) ubiE 320094..320831 (+) 738 WP_003687710.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  OK783_RS01670 (OK783_01670) - 321051..321530 (+) 480 WP_003687711.1 hypothetical protein -
  OK783_RS01675 (OK783_01675) folK 321658..322152 (-) 495 WP_003687713.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 61111.83 Da        Isoelectric Point: 5.0152

>NTDB_id=1485 OK783_RS01640 WP_003687706.1 315526..317199(+) (recN) [Neisseria gonorrhoeae strain FA1090]
MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGLLLGDKADYSQVRSGAKEAQLSALFDISHLPALKAEL
REQGLLDEGGEELSIRRIIDAKGKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDSLAHSAELLQAAEEVGSKIDGDN
GIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAELGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPE
ELPAKLAEIEERLQSLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARFDIVLLPSS
PTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQVPTLIFDEVDTGIGGGVAEMVGKALRALGRK
HQVLAVTHLPQVASCGENHWRVRKHSEGEQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=1485 OK783_RS01640 WP_003687706.1 315526..317199(+) (recN) [Neisseria gonorrhoeae strain FA1090]
ATGCTTCTAACACTTTCTTTGCGTGATTTTGTCATTGTCGAAAATCTGAATCTGGATTTTCAAAGCGGTTTTACCGTTTT
GACCGGCGAGACCGGCGCGGGCAAGTCCATTACTTTGGATGCGATTGGTCTGCTGTTGGGCGATAAGGCCGATTACAGCC
AAGTCCGCAGCGGTGCAAAAGAAGCGCAGCTGTCGGCATTGTTCGATATTTCCCATTTGCCTGCTTTAAAAGCAGAATTG
CGTGAACAAGGGCTGTTGGATGAGGGCGGGGAAGAACTCAGTATCCGCCGCATTATCGATGCCAAAGGCAAAAGCCGCAG
TTTTATCAACAATCAGGCGGCTACCTTGGCGCAACTCAAAGCCGTCGGCGGGCAGCTTATCGACATCCACGGGCAAAACG
ACCATCATTCGCTTAATCAGGAAGCTGCCCAGCGCGAATTGTTGGACGCATTTGTGGGCGGCAGGGTGCAGGCGGAAACC
GTCAGGCAGCTTTATCAAAACTGGGCCAATGCGAAAAAAGCCCTCCAAGAGGCGCAGGAACACGCCGATGCCGTCATTAT
CGAGCGGGAGCGTCTGGAATGGCAGTTTAACGAACTGAATCAGTTGGACATTAAACAAGGCGAATGGGAAGCTCTCAGCC
AAAGCCACGACAGCCTTGCCCATTCTGCCGAATTGTTGCAGGCTGCCGAAGAAGTCGGAAGCAAGATTGACGGCGACAAC
GGCATCCAACGCCATATCTATCAATGTCAAAAACTATTGGCCAATCTGCAAAACATCGAGCCGCGCTTTGCCGAGAGCCT
GAATATGTTGGCAAGCATCGAGGCCGAATTGGGCGAAATCAGTGCCAATATGCGCGATGTGGCAGGACACAGTGACATCA
ACCCCAATGAATTGGCGGCACAGGAACAGCGTATGGGCGAACTGATGGGGATGGCGCGGAAATACCGGATTGAACCCGAA
GAGTTGCCTGCCAAGTTGGCAGAAATCGAAGAACGCCTGCAAAGTCTTCAAGCTGCCGCTGATTTGGACGCGCTCGAGCA
TAATGTTGCCCACAATTTTGCCGAATATCAGGAAGCTGCCCACATCCTTTCTGCCATGCGCCATCAGGCGGCAGGGCGTT
TGAGTAGCGAAACGACCGAGCATATGCAACACCTTGCCATGAAAGGCGCGCGTTTCGACATCGTCCTGTTGCCTTCGTCG
CCGACGGCACACGGTTTGGAGCAGGTTCAATTTCAAGTTGCCGCTAACAAAGGCAATCCGCCCCGCCCGCTGAACAAAGT
CGCCTCCGGCGGCGAATTGGCGCGTATCAGCCTTGCCTTACAGGTTGTTGCCAGCCAATATACCCAAGTTCCCACCCTGA
TTTTTGATGAGGTCGATACCGGTATTGGAGGGGGAGTGGCTGAAATGGTCGGCAAGGCATTACGTGCGTTGGGCAGAAAA
CATCAGGTGCTTGCCGTTACCCATCTTCCGCAAGTCGCATCCTGCGGAGAAAACCACTGGCGGGTGCGCAAGCACAGCGA
GGGAGAGCAAACCGTCAGCGAAATCAGTATATTGGATGAAATACAACGGATCGAAGAGGTTGCCCGTATGTTGGGCGGAG
AAGTTATTACCGATACGACGCGGCAACATGCGGCAGAATTGCTGCAACTTGCGTCGAAAAATAGTTTATTTTAA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(14-508)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Eric P Skaar et al. (2002) Roles of the recJ and recN genes in homologous recombination and DNA repair pathways of Neisseria gonorrhoeae. Journal of Bacteriology 184(4):919-27. [PMID: 11807051]